Streptococcus satellite phage Javan315
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJH5|A0A4D5ZJH5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan315 OX=2558631 GN=JavanS315_0009 PE=4 SV=1
MM1 pKa = 6.87 ITDD4 pKa = 3.77 VEE6 pKa = 4.26 EE7 pKa = 4.04 TTYY10 pKa = 11.07 NGHH13 pKa = 6.47 GEE15 pKa = 3.97 ITVEE19 pKa = 4.03 YY20 pKa = 8.99 TNDD23 pKa = 3.08 VALTEE28 pKa = 4.07 LFDD31 pKa = 5.6 RR32 pKa = 11.84 IKK34 pKa = 10.89 SLYY37 pKa = 9.93 KK38 pKa = 10.28 KK39 pKa = 10.53 GEE41 pKa = 4.11 SYY43 pKa = 7.72 EE44 pKa = 4.32 TKK46 pKa = 10.25 PFLHH50 pKa = 6.71 FKK52 pKa = 10.38 ILEE55 pKa = 4.7 DD56 pKa = 4.67 DD57 pKa = 4.84 LEE59 pKa = 4.48 TKK61 pKa = 10.46 PGVFTAEE68 pKa = 3.68 EE69 pKa = 4.15 VMDD72 pKa = 3.89 FLGII76 pKa = 3.8
Molecular weight: 8.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.423
IPC2_protein 4.406
IPC_protein 4.279
Toseland 4.126
ProMoST 4.38
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.088
Rodwell 4.113
Grimsley 4.037
Solomon 4.215
Lehninger 4.164
Nozaki 4.342
DTASelect 4.457
Thurlkill 4.139
EMBOSS 4.101
Sillero 4.393
Patrickios 3.656
IPC_peptide 4.215
IPC2_peptide 4.38
IPC2.peptide.svr19 4.314
Protein with the highest isoelectric point:
>tr|A0A4D5ZPK2|A0A4D5ZPK2_9VIRU Integrase OS=Streptococcus satellite phage Javan315 OX=2558631 GN=JavanS315_0016 PE=3 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.18 YY6 pKa = 10.54 KK7 pKa = 10.41 KK8 pKa = 10.18 KK9 pKa = 10.4 DD10 pKa = 3.2 GSVVYY15 pKa = 10.34 RR16 pKa = 11.84 SSVYY20 pKa = 10.71 LGIDD24 pKa = 3.15 TVTGKK29 pKa = 10.24 KK30 pKa = 10.26 VKK32 pKa = 8.24 TTISDD37 pKa = 3.44 RR38 pKa = 11.84 TKK40 pKa = 11.03 NRR42 pKa = 11.84 LKK44 pKa = 10.91 SKK46 pKa = 10.43 AIQAKK51 pKa = 9.85 VEE53 pKa = 4.13 FEE55 pKa = 4.56 KK56 pKa = 11.26 NGSTVTKK63 pKa = 8.73 TVNVTTYY70 pKa = 10.96 QEE72 pKa = 4.3 LTEE75 pKa = 4.54 LWLEE79 pKa = 4.43 NYY81 pKa = 9.68 CHH83 pKa = 5.63 TVKK86 pKa = 10.77 HH87 pKa = 5.55 STLIGAKK94 pKa = 10.28 NNIKK98 pKa = 10.37 KK99 pKa = 10.4 YY100 pKa = 10.16 LLPAFGDD107 pKa = 3.76 YY108 pKa = 10.88 KK109 pKa = 10.73 LDD111 pKa = 3.6 KK112 pKa = 9.25 LTPPIIQHH120 pKa = 5.96 QVNQWAIDD128 pKa = 3.66 YY129 pKa = 8.37 NQLGKK134 pKa = 10.84 GYY136 pKa = 8.08 QQYY139 pKa = 10.05 NQLHH143 pKa = 6.4 ALNKK147 pKa = 10.1 RR148 pKa = 11.84 ILSYY152 pKa = 10.8 AVSLQVISSNPASDD166 pKa = 3.94 IIIPRR171 pKa = 11.84 RR172 pKa = 11.84 KK173 pKa = 9.31 PKK175 pKa = 10.29 EE176 pKa = 4.01 GQKK179 pKa = 10.65 LKK181 pKa = 11.15 YY182 pKa = 10.46 LDD184 pKa = 4.52 DD185 pKa = 4.36 NNLKK189 pKa = 10.6 KK190 pKa = 10.67 FLDD193 pKa = 4.02 YY194 pKa = 11.17 LDD196 pKa = 4.36 QLPNTYY202 pKa = 10.72 KK203 pKa = 10.73 NFSDD207 pKa = 3.42 TVLYY211 pKa = 8.14 KK212 pKa = 10.44 TLLATGLRR220 pKa = 11.84 IRR222 pKa = 11.84 EE223 pKa = 4.08 CLALKK228 pKa = 10.05 WSDD231 pKa = 3.05 IDD233 pKa = 3.95 LQNGSISVTKK243 pKa = 9.71 TLNTLKK249 pKa = 10.49 EE250 pKa = 3.91 ITSPKK255 pKa = 8.96 SKK257 pKa = 10.77 SSIRR261 pKa = 11.84 EE262 pKa = 3.4 IALDD266 pKa = 3.68 TKK268 pKa = 9.91 TVLMLRR274 pKa = 11.84 LYY276 pKa = 10.4 KK277 pKa = 10.37 ARR279 pKa = 11.84 QSQIGRR285 pKa = 11.84 EE286 pKa = 3.77 IGVTFEE292 pKa = 4.95 KK293 pKa = 10.88 VFSDD297 pKa = 3.16 TFDD300 pKa = 3.35 NYY302 pKa = 10.97 RR303 pKa = 11.84 EE304 pKa = 4.14 AEE306 pKa = 3.96 ALRR309 pKa = 11.84 FRR311 pKa = 11.84 LEE313 pKa = 3.77 KK314 pKa = 10.18 HH315 pKa = 6.13 LKK317 pKa = 10.08 LAGCPRR323 pKa = 11.84 LSFHH327 pKa = 7.61 AFRR330 pKa = 11.84 HH331 pKa = 4.58 THH333 pKa = 6.92 ASLLLNAGLPYY344 pKa = 10.43 KK345 pKa = 10.2 EE346 pKa = 3.68 IQTRR350 pKa = 11.84 LGHH353 pKa = 5.92 SKK355 pKa = 10.32 ISMTMDD361 pKa = 3.2 IYY363 pKa = 11.76 SHH365 pKa = 7.09 LSKK368 pKa = 10.92 DD369 pKa = 3.71 NKK371 pKa = 10.37 KK372 pKa = 10.32 KK373 pKa = 9.89 ATSFYY378 pKa = 10.66 EE379 pKa = 3.92 KK380 pKa = 10.27 AIEE383 pKa = 4.03 NLQSSS388 pKa = 4.18
Molecular weight: 44.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.033
IPC2_protein 9.194
IPC_protein 9.063
Toseland 9.882
ProMoST 9.545
Dawson 10.087
Bjellqvist 9.736
Wikipedia 10.248
Rodwell 10.613
Grimsley 10.16
Solomon 10.101
Lehninger 10.072
Nozaki 9.882
DTASelect 9.736
Thurlkill 9.94
EMBOSS 10.292
Sillero 9.999
Patrickios 10.014
IPC_peptide 10.101
IPC2_peptide 8.214
IPC2.peptide.svr19 8.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
2612
38
484
163.3
19.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.015 ± 0.384
0.459 ± 0.084
6.547 ± 0.6
8.729 ± 0.897
4.518 ± 0.583
4.977 ± 0.245
1.57 ± 0.254
6.508 ± 0.561
10.26 ± 0.459
9.992 ± 0.804
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.948 ± 0.449
4.786 ± 0.362
2.221 ± 0.26
4.403 ± 0.599
4.9 ± 0.564
5.398 ± 0.577
5.628 ± 0.665
5.092 ± 0.479
0.957 ± 0.122
5.092 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here