Platysternon megacephalum (big-headed turtle)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria;

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21371 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D9F9V8|A0A4D9F9V8_9SAUR [Heparan sulfate]-glucosamine N-sulfotransferase OS=Platysternon megacephalum OX=55544 GN=DR999_PMT00028 PE=3 SV=1
MM1 pKa = 7.9DD2 pKa = 5.47LSFMAAQIPVMGGAFMDD19 pKa = 4.42SPNEE23 pKa = 3.99DD24 pKa = 3.4FGTEE28 pKa = 3.73YY29 pKa = 11.48SLFNSSANVHH39 pKa = 6.31AAASMQNQPEE49 pKa = 4.27EE50 pKa = 4.31TSRR53 pKa = 11.84SSNDD57 pKa = 3.2AIFWLTVSQLQRR69 pKa = 11.84SLIHH73 pKa = 6.92EE74 pKa = 4.09ITGVDD79 pKa = 3.37SQGPFLGAIPYY90 pKa = 9.17SVWLPLQEE98 pKa = 4.19GSAVPFHH105 pKa = 5.43TTEE108 pKa = 3.96PLEE111 pKa = 4.36PDD113 pKa = 3.31SCLDD117 pKa = 4.69CMDD120 pKa = 4.26DD121 pKa = 4.25QSWSICDD128 pKa = 3.32RR129 pKa = 11.84HH130 pKa = 5.74PP131 pKa = 3.93

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D9DP49|A0A4D9DP49_9SAUR Radiation-inducible immediate-early protein IEX-1 OS=Platysternon megacephalum OX=55544 GN=DR999_PMT20021 PE=4 SV=1
MM1 pKa = 7.67AAQSAPKK8 pKa = 10.16VVLKK12 pKa = 10.04STTKK16 pKa = 9.35MSLNEE21 pKa = 4.25RR22 pKa = 11.84FTNMLKK28 pKa = 10.36NKK30 pKa = 9.73QPMPVNIRR38 pKa = 11.84ATMQQQQQLASARR51 pKa = 11.84NRR53 pKa = 11.84RR54 pKa = 11.84LAQQMEE60 pKa = 4.32NRR62 pKa = 11.84PSVQAALKK70 pKa = 10.37LKK72 pKa = 10.39QKK74 pKa = 9.42SLKK77 pKa = 9.72QRR79 pKa = 11.84LGKK82 pKa = 10.77SNIQARR88 pKa = 11.84LGRR91 pKa = 11.84PAGPLARR98 pKa = 11.84GAVGGRR104 pKa = 11.84GLPMGQRR111 pKa = 11.84GLPRR115 pKa = 11.84GAMRR119 pKa = 11.84GGRR122 pKa = 11.84GTRR125 pKa = 11.84ALLRR129 pKa = 11.84GGIPLRR135 pKa = 11.84GQSLLRR141 pKa = 11.84GGRR144 pKa = 11.84GVSPRR149 pKa = 11.84MGLRR153 pKa = 11.84RR154 pKa = 11.84GGVRR158 pKa = 11.84GRR160 pKa = 11.84GGPGRR165 pKa = 11.84GGLGRR170 pKa = 11.84GAMGRR175 pKa = 11.84GGIGGRR181 pKa = 11.84AKK183 pKa = 10.82GPLVSLQHH191 pKa = 6.15LCSS194 pKa = 3.6

Molecular weight: 20.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21371

0

21371

10016199

50

7269

468.7

52.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.947 ± 0.018

2.274 ± 0.014

4.779 ± 0.011

7.086 ± 0.024

3.592 ± 0.012

6.425 ± 0.022

2.54 ± 0.008

4.593 ± 0.014

5.948 ± 0.022

9.788 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.008

3.851 ± 0.014

5.899 ± 0.023

4.794 ± 0.019

5.531 ± 0.015

8.366 ± 0.023

5.353 ± 0.017

5.983 ± 0.014

1.23 ± 0.006

2.747 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski