Capybara microvirus Cap1_SP_127

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W5B3|A0A4P8W5B3_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_127 OX=2585380 PE=4 SV=1
MM1 pKa = 7.79TYY3 pKa = 11.04NDD5 pKa = 3.94YY6 pKa = 10.74LQQYY10 pKa = 9.22AHH12 pKa = 7.48LFHH15 pKa = 7.08EE16 pKa = 5.45KK17 pKa = 9.86YY18 pKa = 10.7DD19 pKa = 3.77KK20 pKa = 11.03VRR22 pKa = 11.84NTIPDD27 pKa = 3.85AVPSDD32 pKa = 4.0SQLVAQFTGKK42 pKa = 10.06VPLTSNTGQIGVQKK56 pKa = 10.42EE57 pKa = 4.81SYY59 pKa = 9.45QSTYY63 pKa = 11.01QEE65 pKa = 4.12VNLDD69 pKa = 3.91NILPEE74 pKa = 4.37SKK76 pKa = 10.59LYY78 pKa = 8.06MTFEE82 pKa = 4.78DD83 pKa = 4.04AFQNLRR89 pKa = 11.84DD90 pKa = 4.07GGNAATNLRR99 pKa = 11.84DD100 pKa = 3.36LNEE103 pKa = 4.61YY104 pKa = 7.93YY105 pKa = 9.63TQLKK109 pKa = 10.19NKK111 pKa = 9.59EE112 pKa = 3.91KK113 pKa = 10.69SQNLNPADD121 pKa = 3.95GSTATSSNQDD131 pKa = 2.8NKK133 pKa = 11.39

Molecular weight: 15.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W474|A0A4P8W474_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_127 OX=2585380 PE=4 SV=1
MM1 pKa = 7.27NCLHH5 pKa = 6.6PVDD8 pKa = 4.53VRR10 pKa = 11.84ITRR13 pKa = 11.84KK14 pKa = 10.44DD15 pKa = 3.12GTLIPALVPCGKK27 pKa = 10.39CYY29 pKa = 10.58ACQYY33 pKa = 8.98NDD35 pKa = 3.38RR36 pKa = 11.84VGWSYY41 pKa = 11.35RR42 pKa = 11.84ATLEE46 pKa = 4.43SNSSKK51 pKa = 10.86YY52 pKa = 10.91SLFVTLTYY60 pKa = 11.46NNLHH64 pKa = 6.66LDD66 pKa = 3.64KK67 pKa = 10.01LTHH70 pKa = 6.14NGKK73 pKa = 10.3SYY75 pKa = 10.91GVVSRR80 pKa = 11.84EE81 pKa = 3.63TLQKK85 pKa = 10.36FFKK88 pKa = 10.08RR89 pKa = 11.84CRR91 pKa = 11.84KK92 pKa = 9.69RR93 pKa = 11.84GLKK96 pKa = 9.98FSYY99 pKa = 10.19IFVSEE104 pKa = 4.08YY105 pKa = 11.19GPLTNRR111 pKa = 11.84PHH113 pKa = 4.98YY114 pKa = 10.36HH115 pKa = 6.8GIFFLHH121 pKa = 6.4QDD123 pKa = 3.19NTFEE127 pKa = 5.5LDD129 pKa = 4.43DD130 pKa = 5.0IIEE133 pKa = 4.87KK134 pKa = 10.24ISKK137 pKa = 9.16SWNLGFSKK145 pKa = 10.16ISEE148 pKa = 4.22VTPARR153 pKa = 11.84INYY156 pKa = 5.23VTGYY160 pKa = 9.76SFKK163 pKa = 10.47EE164 pKa = 4.15CNPKK168 pKa = 10.25FSFQTPTFRR177 pKa = 11.84LMSRR181 pKa = 11.84RR182 pKa = 11.84PAIGYY187 pKa = 9.01DD188 pKa = 3.69LLADD192 pKa = 3.78EE193 pKa = 4.31QFKK196 pKa = 11.15NISNHH201 pKa = 5.31IANGGNSLRR210 pKa = 11.84FGSASIPIPKK220 pKa = 9.75YY221 pKa = 10.42YY222 pKa = 10.31KK223 pKa = 9.08NKK225 pKa = 9.71LRR227 pKa = 11.84KK228 pKa = 8.86EE229 pKa = 4.11HH230 pKa = 6.52TNDD233 pKa = 3.08EE234 pKa = 4.38LFNIYY239 pKa = 10.27NSQITRR245 pKa = 11.84QNLRR249 pKa = 11.84TKK251 pKa = 10.64KK252 pKa = 10.07LFEE255 pKa = 4.27YY256 pKa = 9.98FQSKK260 pKa = 9.44YY261 pKa = 10.22PKK263 pKa = 9.84LSPSRR268 pKa = 11.84IYY270 pKa = 11.11SLIYY274 pKa = 10.51SDD276 pKa = 5.92DD277 pKa = 3.71DD278 pKa = 3.7TNLRR282 pKa = 11.84LKK284 pKa = 10.6RR285 pKa = 11.84QALKK289 pKa = 10.86NIYY292 pKa = 10.14KK293 pKa = 10.08NLKK296 pKa = 9.39NNEE299 pKa = 4.16DD300 pKa = 3.63NFNN303 pKa = 3.58

Molecular weight: 35.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1575

69

636

262.5

30.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.254 ± 0.594

1.143 ± 0.595

5.587 ± 0.577

5.016 ± 0.313

6.095 ± 1.03

4.889 ± 0.429

1.651 ± 0.32

5.841 ± 0.906

7.111 ± 1.691

9.143 ± 0.61

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.587 ± 0.329

8.571 ± 0.989

4.635 ± 0.709

5.016 ± 1.167

4.508 ± 0.775

7.937 ± 0.394

6.222 ± 0.775

4.127 ± 0.732

0.762 ± 0.153

5.905 ± 0.925
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski