Amino acid dipepetide frequency for Leuconostoc phage 1-A4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.887AlaAla: 0.887 ± 0.384
0.222AlaCys: 0.222 ± 0.162
4.434AlaAsp: 4.434 ± 0.819
3.547AlaGlu: 3.547 ± 0.695
2.993AlaPhe: 2.993 ± 0.995
4.434AlaGly: 4.434 ± 0.974
1.219AlaHis: 1.219 ± 0.364
5.099AlaIle: 5.099 ± 0.965
4.212AlaLys: 4.212 ± 0.691
5.431AlaLeu: 5.431 ± 0.712
1.441AlaMet: 1.441 ± 0.342
4.101AlaAsn: 4.101 ± 0.672
1.219AlaPro: 1.219 ± 0.328
1.884AlaGln: 1.884 ± 0.47
1.441AlaArg: 1.441 ± 0.503
4.101AlaSer: 4.101 ± 1.012
4.101AlaThr: 4.101 ± 0.742
4.323AlaVal: 4.323 ± 0.559
0.998AlaTrp: 0.998 ± 0.417
2.217AlaTyr: 2.217 ± 0.415
0.0AlaXaa: 0.0 ± 0.0
Cys
0.111CysAla: 0.111 ± 0.098
0.0CysCys: 0.0 ± 0.0
0.111CysAsp: 0.111 ± 0.114
0.333CysGlu: 0.333 ± 0.17
0.222CysPhe: 0.222 ± 0.153
0.333CysGly: 0.333 ± 0.28
0.333CysHis: 0.333 ± 0.187
0.0CysIle: 0.0 ± 0.0
0.111CysLys: 0.111 ± 0.131
0.222CysLeu: 0.222 ± 0.171
0.111CysMet: 0.111 ± 0.131
0.111CysAsn: 0.111 ± 0.118
0.111CysPro: 0.111 ± 0.109
0.111CysGln: 0.111 ± 0.145
0.222CysArg: 0.222 ± 0.192
0.554CysSer: 0.554 ± 0.259
0.0CysThr: 0.0 ± 0.0
0.111CysVal: 0.111 ± 0.12
0.111CysTrp: 0.111 ± 0.109
0.111CysTyr: 0.111 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
3.658AspAla: 3.658 ± 0.857
0.222AspCys: 0.222 ± 0.167
4.212AspAsp: 4.212 ± 0.844
5.653AspGlu: 5.653 ± 0.9
3.325AspPhe: 3.325 ± 0.519
4.655AspGly: 4.655 ± 0.719
0.887AspHis: 0.887 ± 0.299
4.101AspIle: 4.101 ± 0.602
5.209AspLys: 5.209 ± 0.748
5.431AspLeu: 5.431 ± 0.73
1.219AspMet: 1.219 ± 0.331
4.434AspAsn: 4.434 ± 0.825
1.884AspPro: 1.884 ± 0.412
1.219AspGln: 1.219 ± 0.333
1.773AspArg: 1.773 ± 0.47
3.436AspSer: 3.436 ± 0.616
4.323AspThr: 4.323 ± 0.634
4.434AspVal: 4.434 ± 0.569
1.219AspTrp: 1.219 ± 0.371
4.101AspTyr: 4.101 ± 0.989
0.0AspXaa: 0.0 ± 0.0
Glu
2.438GluAla: 2.438 ± 0.541
0.333GluCys: 0.333 ± 0.195
4.323GluAsp: 4.323 ± 0.789
4.877GluGlu: 4.877 ± 0.998
2.993GluPhe: 2.993 ± 0.642
2.217GluGly: 2.217 ± 0.517
0.887GluHis: 0.887 ± 0.287
4.544GluIle: 4.544 ± 0.898
5.209GluLys: 5.209 ± 0.872
6.65GluLeu: 6.65 ± 0.867
3.658GluMet: 3.658 ± 0.733
4.655GluAsn: 4.655 ± 0.673
1.33GluPro: 1.33 ± 0.447
3.436GluGln: 3.436 ± 0.719
2.106GluArg: 2.106 ± 0.447
2.993GluSer: 2.993 ± 0.645
3.879GluThr: 3.879 ± 0.728
5.542GluVal: 5.542 ± 0.777
0.887GluTrp: 0.887 ± 0.305
2.993GluTyr: 2.993 ± 0.672
0.0GluXaa: 0.0 ± 0.0
Phe
2.106PheAla: 2.106 ± 0.449
0.111PheCys: 0.111 ± 0.12
3.99PheAsp: 3.99 ± 0.681
3.436PheGlu: 3.436 ± 0.604
1.219PhePhe: 1.219 ± 0.41
3.214PheGly: 3.214 ± 0.552
0.222PheHis: 0.222 ± 0.16
4.877PheIle: 4.877 ± 0.716
2.882PheLys: 2.882 ± 0.547
3.214PheLeu: 3.214 ± 0.512
0.776PheMet: 0.776 ± 0.306
2.771PheAsn: 2.771 ± 0.572
1.219PhePro: 1.219 ± 0.483
1.108PheGln: 1.108 ± 0.302
1.552PheArg: 1.552 ± 0.364
4.544PheSer: 4.544 ± 1.445
3.547PheThr: 3.547 ± 0.673
2.66PheVal: 2.66 ± 0.4
0.665PheTrp: 0.665 ± 0.294
2.106PheTyr: 2.106 ± 0.541
0.0PheXaa: 0.0 ± 0.0
Gly
3.436GlyAla: 3.436 ± 0.909
0.111GlyCys: 0.111 ± 0.132
4.655GlyAsp: 4.655 ± 0.627
2.882GlyGlu: 2.882 ± 0.463
3.99GlyPhe: 3.99 ± 0.629
4.988GlyGly: 4.988 ± 0.783
0.776GlyHis: 0.776 ± 0.309
5.209GlyIle: 5.209 ± 1.096
5.431GlyLys: 5.431 ± 0.758
4.877GlyLeu: 4.877 ± 0.728
2.549GlyMet: 2.549 ± 0.593
5.099GlyAsn: 5.099 ± 1.031
0.222GlyPro: 0.222 ± 0.155
2.771GlyGln: 2.771 ± 0.483
2.106GlyArg: 2.106 ± 0.503
5.431GlySer: 5.431 ± 0.912
3.99GlyThr: 3.99 ± 0.701
4.323GlyVal: 4.323 ± 0.914
0.665GlyTrp: 0.665 ± 0.232
3.325GlyTyr: 3.325 ± 0.626
0.0GlyXaa: 0.0 ± 0.0
His
0.665HisAla: 0.665 ± 0.314
0.111HisCys: 0.111 ± 0.112
0.554HisAsp: 0.554 ± 0.215
0.887HisGlu: 0.887 ± 0.329
0.554HisPhe: 0.554 ± 0.269
0.554HisGly: 0.554 ± 0.252
0.443HisHis: 0.443 ± 0.237
1.108HisIle: 1.108 ± 0.329
0.443HisLys: 0.443 ± 0.222
1.108HisLeu: 1.108 ± 0.51
0.665HisMet: 0.665 ± 0.287
0.665HisAsn: 0.665 ± 0.293
0.554HisPro: 0.554 ± 0.239
0.887HisGln: 0.887 ± 0.414
0.776HisArg: 0.776 ± 0.263
0.887HisSer: 0.887 ± 0.274
1.108HisThr: 1.108 ± 0.482
0.887HisVal: 0.887 ± 0.363
0.0HisTrp: 0.0 ± 0.0
0.998HisTyr: 0.998 ± 0.318
0.0HisXaa: 0.0 ± 0.0
Ile
3.879IleAla: 3.879 ± 0.568
0.222IleCys: 0.222 ± 0.16
4.766IleAsp: 4.766 ± 0.63
6.761IleGlu: 6.761 ± 1.115
2.882IlePhe: 2.882 ± 0.421
4.655IleGly: 4.655 ± 0.993
0.998IleHis: 0.998 ± 0.344
5.099IleIle: 5.099 ± 0.794
5.764IleLys: 5.764 ± 0.907
4.988IleLeu: 4.988 ± 0.817
1.884IleMet: 1.884 ± 0.452
3.879IleAsn: 3.879 ± 0.484
2.106IlePro: 2.106 ± 0.457
2.771IleGln: 2.771 ± 0.594
1.995IleArg: 1.995 ± 0.403
4.988IleSer: 4.988 ± 0.824
5.653IleThr: 5.653 ± 0.938
4.323IleVal: 4.323 ± 0.545
0.111IleTrp: 0.111 ± 0.11
2.217IleTyr: 2.217 ± 0.52
0.0IleXaa: 0.0 ± 0.0
Lys
5.099LysAla: 5.099 ± 0.803
0.222LysCys: 0.222 ± 0.171
4.323LysAsp: 4.323 ± 0.762
5.32LysGlu: 5.32 ± 0.852
3.214LysPhe: 3.214 ± 0.599
3.769LysGly: 3.769 ± 0.584
1.552LysHis: 1.552 ± 0.512
4.877LysIle: 4.877 ± 0.645
6.207LysLys: 6.207 ± 1.038
6.983LysLeu: 6.983 ± 1.147
2.771LysMet: 2.771 ± 0.559
4.212LysAsn: 4.212 ± 0.606
1.995LysPro: 1.995 ± 0.483
2.882LysGln: 2.882 ± 0.497
3.547LysArg: 3.547 ± 0.663
4.212LysSer: 4.212 ± 0.57
5.099LysThr: 5.099 ± 0.59
3.879LysVal: 3.879 ± 0.755
0.887LysTrp: 0.887 ± 0.309
3.879LysTyr: 3.879 ± 0.736
0.0LysXaa: 0.0 ± 0.0
Leu
5.099LeuAla: 5.099 ± 0.853
0.222LeuCys: 0.222 ± 0.165
6.429LeuAsp: 6.429 ± 0.79
5.985LeuGlu: 5.985 ± 1.008
3.214LeuPhe: 3.214 ± 0.562
5.985LeuGly: 5.985 ± 1.117
0.998LeuHis: 0.998 ± 0.337
4.544LeuIle: 4.544 ± 0.805
7.759LeuLys: 7.759 ± 1.011
5.209LeuLeu: 5.209 ± 0.877
2.328LeuMet: 2.328 ± 0.415
5.542LeuAsn: 5.542 ± 0.671
2.993LeuPro: 2.993 ± 0.565
3.325LeuGln: 3.325 ± 0.561
1.773LeuArg: 1.773 ± 0.374
6.761LeuSer: 6.761 ± 1.134
6.429LeuThr: 6.429 ± 0.911
4.655LeuVal: 4.655 ± 0.83
0.554LeuTrp: 0.554 ± 0.227
2.217LeuTyr: 2.217 ± 0.439
0.0LeuXaa: 0.0 ± 0.0
Met
2.438MetAla: 2.438 ± 0.49
0.111MetCys: 0.111 ± 0.109
0.887MetAsp: 0.887 ± 0.328
1.108MetGlu: 1.108 ± 0.299
1.441MetPhe: 1.441 ± 0.415
1.219MetGly: 1.219 ± 0.331
0.333MetHis: 0.333 ± 0.211
1.663MetIle: 1.663 ± 0.471
1.995MetLys: 1.995 ± 0.43
1.884MetLeu: 1.884 ± 0.384
0.665MetMet: 0.665 ± 0.244
2.66MetAsn: 2.66 ± 0.606
0.554MetPro: 0.554 ± 0.291
0.443MetGln: 0.443 ± 0.206
0.887MetArg: 0.887 ± 0.278
2.328MetSer: 2.328 ± 0.449
2.549MetThr: 2.549 ± 0.542
2.328MetVal: 2.328 ± 0.533
0.333MetTrp: 0.333 ± 0.174
1.441MetTyr: 1.441 ± 0.401
0.0MetXaa: 0.0 ± 0.0
Asn
4.101AsnAla: 4.101 ± 0.705
0.222AsnCys: 0.222 ± 0.178
3.104AsnAsp: 3.104 ± 0.499
4.655AsnGlu: 4.655 ± 0.69
3.325AsnPhe: 3.325 ± 0.669
5.099AsnGly: 5.099 ± 0.79
0.554AsnHis: 0.554 ± 0.29
4.434AsnIle: 4.434 ± 0.561
4.323AsnLys: 4.323 ± 0.697
4.212AsnLeu: 4.212 ± 1.042
0.887AsnMet: 0.887 ± 0.311
4.766AsnAsn: 4.766 ± 0.742
2.549AsnPro: 2.549 ± 0.557
1.663AsnGln: 1.663 ± 0.374
2.882AsnArg: 2.882 ± 0.604
4.766AsnSer: 4.766 ± 0.653
4.877AsnThr: 4.877 ± 0.655
4.544AsnVal: 4.544 ± 0.532
0.554AsnTrp: 0.554 ± 0.215
3.436AsnTyr: 3.436 ± 0.73
0.0AsnXaa: 0.0 ± 0.0
Pro
1.773ProAla: 1.773 ± 0.476
0.0ProCys: 0.0 ± 0.0
1.884ProAsp: 1.884 ± 0.419
1.884ProGlu: 1.884 ± 0.507
1.33ProPhe: 1.33 ± 0.343
0.887ProGly: 0.887 ± 0.373
0.443ProHis: 0.443 ± 0.243
1.884ProIle: 1.884 ± 0.442
2.217ProLys: 2.217 ± 0.493
1.552ProLeu: 1.552 ± 0.305
0.998ProMet: 0.998 ± 0.342
2.438ProAsn: 2.438 ± 0.64
0.443ProPro: 0.443 ± 0.191
0.776ProGln: 0.776 ± 0.352
0.887ProArg: 0.887 ± 0.373
1.884ProSer: 1.884 ± 0.421
2.328ProThr: 2.328 ± 0.592
1.995ProVal: 1.995 ± 0.433
0.0ProTrp: 0.0 ± 0.0
1.219ProTyr: 1.219 ± 0.373
0.0ProXaa: 0.0 ± 0.0
Gln
2.882GlnAla: 2.882 ± 0.655
0.0GlnCys: 0.0 ± 0.0
2.106GlnAsp: 2.106 ± 0.524
1.995GlnGlu: 1.995 ± 0.455
1.663GlnPhe: 1.663 ± 0.445
1.773GlnGly: 1.773 ± 0.436
0.443GlnHis: 0.443 ± 0.282
2.66GlnIle: 2.66 ± 0.484
3.436GlnLys: 3.436 ± 0.666
3.436GlnLeu: 3.436 ± 0.681
0.998GlnMet: 0.998 ± 0.278
1.995GlnAsn: 1.995 ± 0.416
0.887GlnPro: 0.887 ± 0.309
1.663GlnGln: 1.663 ± 0.464
1.441GlnArg: 1.441 ± 0.423
2.438GlnSer: 2.438 ± 0.545
2.328GlnThr: 2.328 ± 0.582
2.66GlnVal: 2.66 ± 0.407
0.443GlnTrp: 0.443 ± 0.259
1.108GlnTyr: 1.108 ± 0.43
0.0GlnXaa: 0.0 ± 0.0
Arg
1.995ArgAla: 1.995 ± 0.386
0.333ArgCys: 0.333 ± 0.248
2.549ArgAsp: 2.549 ± 0.43
1.884ArgGlu: 1.884 ± 0.584
1.441ArgPhe: 1.441 ± 0.479
1.995ArgGly: 1.995 ± 0.492
0.333ArgHis: 0.333 ± 0.204
2.328ArgIle: 2.328 ± 0.503
1.995ArgLys: 1.995 ± 0.44
5.209ArgLeu: 5.209 ± 0.563
0.776ArgMet: 0.776 ± 0.288
1.663ArgAsn: 1.663 ± 0.472
1.108ArgPro: 1.108 ± 0.432
1.33ArgGln: 1.33 ± 0.341
1.33ArgArg: 1.33 ± 0.536
1.995ArgSer: 1.995 ± 0.452
2.882ArgThr: 2.882 ± 0.56
2.549ArgVal: 2.549 ± 0.537
0.665ArgTrp: 0.665 ± 0.231
0.887ArgTyr: 0.887 ± 0.334
0.0ArgXaa: 0.0 ± 0.0
Ser
4.877SerAla: 4.877 ± 1.211
0.0SerCys: 0.0 ± 0.0
4.434SerAsp: 4.434 ± 0.753
3.325SerGlu: 3.325 ± 0.543
3.99SerPhe: 3.99 ± 0.67
6.872SerGly: 6.872 ± 1.362
0.776SerHis: 0.776 ± 0.258
5.099SerIle: 5.099 ± 0.77
4.101SerLys: 4.101 ± 0.683
4.877SerLeu: 4.877 ± 0.931
1.108SerMet: 1.108 ± 0.36
4.323SerAsn: 4.323 ± 0.67
1.219SerPro: 1.219 ± 0.391
2.771SerGln: 2.771 ± 0.504
2.106SerArg: 2.106 ± 0.506
5.985SerSer: 5.985 ± 1.59
5.542SerThr: 5.542 ± 0.768
4.988SerVal: 4.988 ± 0.968
0.333SerTrp: 0.333 ± 0.17
2.993SerTyr: 2.993 ± 0.533
0.0SerXaa: 0.0 ± 0.0
Thr
4.766ThrAla: 4.766 ± 0.85
0.111ThrCys: 0.111 ± 0.131
3.436ThrAsp: 3.436 ± 0.616
3.99ThrGlu: 3.99 ± 0.721
3.769ThrPhe: 3.769 ± 0.597
5.764ThrGly: 5.764 ± 0.792
0.887ThrHis: 0.887 ± 0.319
4.655ThrIle: 4.655 ± 0.683
5.764ThrLys: 5.764 ± 0.767
6.207ThrLeu: 6.207 ± 0.864
1.663ThrMet: 1.663 ± 0.472
4.434ThrAsn: 4.434 ± 0.734
2.882ThrPro: 2.882 ± 0.624
3.658ThrGln: 3.658 ± 0.782
3.436ThrArg: 3.436 ± 0.563
4.434ThrSer: 4.434 ± 0.719
6.096ThrThr: 6.096 ± 1.203
3.658ThrVal: 3.658 ± 0.619
0.665ThrTrp: 0.665 ± 0.29
1.884ThrTyr: 1.884 ± 0.423
0.0ThrXaa: 0.0 ± 0.0
Val
5.32ValAla: 5.32 ± 0.897
0.333ValCys: 0.333 ± 0.203
4.655ValAsp: 4.655 ± 0.613
3.325ValGlu: 3.325 ± 0.506
2.549ValPhe: 2.549 ± 0.457
4.877ValGly: 4.877 ± 0.837
0.665ValHis: 0.665 ± 0.259
4.655ValIle: 4.655 ± 0.731
3.99ValLys: 3.99 ± 0.618
5.209ValLeu: 5.209 ± 0.703
1.552ValMet: 1.552 ± 0.485
4.323ValAsn: 4.323 ± 0.689
2.106ValPro: 2.106 ± 0.477
1.33ValGln: 1.33 ± 0.466
3.214ValArg: 3.214 ± 0.592
4.101ValSer: 4.101 ± 0.562
4.434ValThr: 4.434 ± 0.637
4.101ValVal: 4.101 ± 0.796
1.552ValTrp: 1.552 ± 0.344
3.658ValTyr: 3.658 ± 0.572
0.0ValXaa: 0.0 ± 0.0
Trp
0.443TrpAla: 0.443 ± 0.189
0.0TrpCys: 0.0 ± 0.0
1.219TrpAsp: 1.219 ± 0.37
0.554TrpGlu: 0.554 ± 0.257
0.222TrpPhe: 0.222 ± 0.164
0.665TrpGly: 0.665 ± 0.297
0.554TrpHis: 0.554 ± 0.266
0.222TrpIle: 0.222 ± 0.166
0.222TrpLys: 0.222 ± 0.155
0.998TrpLeu: 0.998 ± 0.253
0.333TrpMet: 0.333 ± 0.175
0.443TrpAsn: 0.443 ± 0.227
0.111TrpPro: 0.111 ± 0.109
0.665TrpGln: 0.665 ± 0.243
0.665TrpArg: 0.665 ± 0.252
0.998TrpSer: 0.998 ± 0.356
0.554TrpThr: 0.554 ± 0.211
0.776TrpVal: 0.776 ± 0.289
0.222TrpTrp: 0.222 ± 0.157
1.108TrpTyr: 1.108 ± 0.423
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.328TyrAla: 2.328 ± 0.41
0.443TyrCys: 0.443 ± 0.233
3.325TyrAsp: 3.325 ± 0.691
3.547TyrGlu: 3.547 ± 0.748
1.884TyrPhe: 1.884 ± 0.423
2.882TyrGly: 2.882 ± 0.633
0.554TyrHis: 0.554 ± 0.246
2.882TyrIle: 2.882 ± 0.479
3.769TyrLys: 3.769 ± 0.749
4.323TyrLeu: 4.323 ± 0.727
0.776TyrMet: 0.776 ± 0.292
2.438TyrAsn: 2.438 ± 0.533
1.33TyrPro: 1.33 ± 0.331
1.663TyrGln: 1.663 ± 0.378
1.108TyrArg: 1.108 ± 0.377
2.882TyrSer: 2.882 ± 0.828
2.438TyrThr: 2.438 ± 0.524
3.214TyrVal: 3.214 ± 0.662
0.111TyrTrp: 0.111 ± 0.114
1.884TyrTyr: 1.884 ± 0.396
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (9023 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski