Amino acid dipepetide frequency for Streptomyces sp. e14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.095AlaAla: 22.095 ± 0.152
1.124AlaCys: 1.124 ± 0.023
8.486AlaAsp: 8.486 ± 0.068
8.865AlaGlu: 8.865 ± 0.089
3.447AlaPhe: 3.447 ± 0.045
13.692AlaGly: 13.692 ± 0.118
3.105AlaHis: 3.105 ± 0.042
3.226AlaIle: 3.226 ± 0.05
3.013AlaLys: 3.013 ± 0.062
14.343AlaLeu: 14.343 ± 0.119
2.434AlaMet: 2.434 ± 0.036
1.864AlaAsn: 1.864 ± 0.036
7.743AlaPro: 7.743 ± 0.084
3.763AlaGln: 3.763 ± 0.046
11.069AlaArg: 11.069 ± 0.104
5.938AlaSer: 5.938 ± 0.057
6.851AlaThr: 6.851 ± 0.067
12.365AlaVal: 12.365 ± 0.097
1.796AlaTrp: 1.796 ± 0.03
2.894AlaTyr: 2.894 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.214CysAla: 1.214 ± 0.027
0.13CysCys: 0.13 ± 0.008
0.485CysAsp: 0.485 ± 0.015
0.394CysGlu: 0.394 ± 0.015
0.221CysPhe: 0.221 ± 0.012
0.988CysGly: 0.988 ± 0.025
0.197CysHis: 0.197 ± 0.011
0.152CysIle: 0.152 ± 0.008
0.117CysLys: 0.117 ± 0.008
0.742CysLeu: 0.742 ± 0.02
0.131CysMet: 0.131 ± 0.009
0.121CysAsn: 0.121 ± 0.009
0.534CysPro: 0.534 ± 0.014
0.155CysGln: 0.155 ± 0.009
0.67CysArg: 0.67 ± 0.018
0.458CysSer: 0.458 ± 0.017
0.51CysThr: 0.51 ± 0.017
0.691CysVal: 0.691 ± 0.018
0.13CysTrp: 0.13 ± 0.008
0.157CysTyr: 0.157 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.82AspAla: 7.82 ± 0.071
0.414AspCys: 0.414 ± 0.017
3.526AspAsp: 3.526 ± 0.049
3.713AspGlu: 3.713 ± 0.054
1.67AspPhe: 1.67 ± 0.029
6.397AspGly: 6.397 ± 0.063
1.478AspHis: 1.478 ± 0.031
1.824AspIle: 1.824 ± 0.034
1.228AspLys: 1.228 ± 0.028
6.07AspLeu: 6.07 ± 0.064
0.804AspMet: 0.804 ± 0.021
0.92AspAsn: 0.92 ± 0.025
4.456AspPro: 4.456 ± 0.052
1.545AspGln: 1.545 ± 0.031
5.155AspArg: 5.155 ± 0.061
2.495AspSer: 2.495 ± 0.038
3.137AspThr: 3.137 ± 0.047
4.694AspVal: 4.694 ± 0.048
0.983AspTrp: 0.983 ± 0.022
1.134AspTyr: 1.134 ± 0.027
0.0AspXaa: 0.0 ± 0.0
Glu
7.189GluAla: 7.189 ± 0.079
0.369GluCys: 0.369 ± 0.014
2.877GluAsp: 2.877 ± 0.042
3.527GluGlu: 3.527 ± 0.049
1.453GluPhe: 1.453 ± 0.032
4.199GluGly: 4.199 ± 0.06
1.562GluHis: 1.562 ± 0.028
2.26GluIle: 2.26 ± 0.038
1.423GluLys: 1.423 ± 0.029
6.572GluLeu: 6.572 ± 0.071
0.836GluMet: 0.836 ± 0.024
0.989GluAsn: 0.989 ± 0.022
3.326GluPro: 3.326 ± 0.043
2.152GluGln: 2.152 ± 0.034
5.598GluArg: 5.598 ± 0.064
2.375GluSer: 2.375 ± 0.035
2.857GluThr: 2.857 ± 0.037
4.325GluVal: 4.325 ± 0.052
0.69GluTrp: 0.69 ± 0.019
1.091GluTyr: 1.091 ± 0.019
0.0GluXaa: 0.0 ± 0.0
Phe
3.72PheAla: 3.72 ± 0.049
0.256PheCys: 0.256 ± 0.012
1.911PheAsp: 1.911 ± 0.033
1.412PheGlu: 1.412 ± 0.029
0.869PhePhe: 0.869 ± 0.023
2.96PheGly: 2.96 ± 0.047
0.638PheHis: 0.638 ± 0.02
0.642PheIle: 0.642 ± 0.017
0.533PheLys: 0.533 ± 0.017
2.567PheLeu: 2.567 ± 0.037
0.383PheMet: 0.383 ± 0.015
0.531PheAsn: 0.531 ± 0.016
1.4PhePro: 1.4 ± 0.027
0.683PheGln: 0.683 ± 0.019
1.851PheArg: 1.851 ± 0.035
1.438PheSer: 1.438 ± 0.026
1.963PheThr: 1.963 ± 0.033
2.248PheVal: 2.248 ± 0.039
0.4PheTrp: 0.4 ± 0.016
0.579PheTyr: 0.579 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
11.901GlyAla: 11.901 ± 0.102
0.86GlyCys: 0.86 ± 0.023
5.349GlyAsp: 5.349 ± 0.063
5.036GlyGlu: 5.036 ± 0.056
2.817GlyPhe: 2.817 ± 0.038
9.536GlyGly: 9.536 ± 0.105
2.502GlyHis: 2.502 ± 0.036
3.199GlyIle: 3.199 ± 0.045
2.458GlyLys: 2.458 ± 0.047
9.097GlyLeu: 9.097 ± 0.073
1.952GlyMet: 1.952 ± 0.034
1.723GlyAsn: 1.723 ± 0.037
5.536GlyPro: 5.536 ± 0.068
2.679GlyGln: 2.679 ± 0.049
8.779GlyArg: 8.779 ± 0.083
5.219GlySer: 5.219 ± 0.064
6.168GlyThr: 6.168 ± 0.075
7.651GlyVal: 7.651 ± 0.066
1.625GlyTrp: 1.625 ± 0.026
2.285GlyTyr: 2.285 ± 0.031
0.0GlyXaa: 0.0 ± 0.0
His
2.743HisAla: 2.743 ± 0.04
0.213HisCys: 0.213 ± 0.011
1.381HisAsp: 1.381 ± 0.027
1.253HisGlu: 1.253 ± 0.026
0.664HisPhe: 0.664 ± 0.02
2.6HisGly: 2.6 ± 0.042
0.742HisHis: 0.742 ± 0.021
0.671HisIle: 0.671 ± 0.019
0.374HisLys: 0.374 ± 0.015
2.384HisLeu: 2.384 ± 0.04
0.342HisMet: 0.342 ± 0.014
0.348HisAsn: 0.348 ± 0.014
1.981HisPro: 1.981 ± 0.033
0.665HisGln: 0.665 ± 0.018
2.381HisArg: 2.381 ± 0.036
1.02HisSer: 1.02 ± 0.025
1.398HisThr: 1.398 ± 0.03
1.715HisVal: 1.715 ± 0.029
0.379HisTrp: 0.379 ± 0.015
0.492HisTyr: 0.492 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.575IleAla: 4.575 ± 0.053
0.274IleCys: 0.274 ± 0.011
2.164IleAsp: 2.164 ± 0.036
1.91IleGlu: 1.91 ± 0.036
0.644IlePhe: 0.644 ± 0.02
3.43IleGly: 3.43 ± 0.044
0.577IleHis: 0.577 ± 0.017
0.804IleIle: 0.804 ± 0.02
0.733IleLys: 0.733 ± 0.024
2.282IleLeu: 2.282 ± 0.04
0.439IleMet: 0.439 ± 0.017
0.63IleAsn: 0.63 ± 0.02
1.641IlePro: 1.641 ± 0.028
0.713IleGln: 0.713 ± 0.02
2.131IleArg: 2.131 ± 0.033
1.571IleSer: 1.571 ± 0.032
2.087IleThr: 2.087 ± 0.04
2.694IleVal: 2.694 ± 0.038
0.358IleTrp: 0.358 ± 0.014
0.493IleTyr: 0.493 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
3.028LysAla: 3.028 ± 0.058
0.128LysCys: 0.128 ± 0.009
1.365LysAsp: 1.365 ± 0.033
1.2LysGlu: 1.2 ± 0.03
0.488LysPhe: 0.488 ± 0.016
1.872LysGly: 1.872 ± 0.038
0.42LysHis: 0.42 ± 0.015
0.917LysIle: 0.917 ± 0.025
0.928LysLys: 0.928 ± 0.035
2.025LysLeu: 2.025 ± 0.034
0.393LysMet: 0.393 ± 0.013
0.564LysAsn: 0.564 ± 0.02
1.3LysPro: 1.3 ± 0.028
0.723LysGln: 0.723 ± 0.021
1.453LysArg: 1.453 ± 0.033
1.151LysSer: 1.151 ± 0.027
1.347LysThr: 1.347 ± 0.031
1.915LysVal: 1.915 ± 0.039
0.272LysTrp: 0.272 ± 0.012
0.484LysTyr: 0.484 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
14.808LeuAla: 14.808 ± 0.126
0.826LeuCys: 0.826 ± 0.021
6.542LeuAsp: 6.542 ± 0.073
4.591LeuGlu: 4.591 ± 0.061
2.599LeuPhe: 2.599 ± 0.04
8.94LeuGly: 8.94 ± 0.074
2.29LeuHis: 2.29 ± 0.033
3.112LeuIle: 3.112 ± 0.047
2.144LeuLys: 2.144 ± 0.037
10.882LeuLeu: 10.882 ± 0.114
1.59LeuMet: 1.59 ± 0.034
1.627LeuAsn: 1.627 ± 0.031
6.495LeuPro: 6.495 ± 0.064
2.176LeuGln: 2.176 ± 0.032
8.739LeuArg: 8.739 ± 0.084
4.988LeuSer: 4.988 ± 0.057
6.733LeuThr: 6.733 ± 0.064
8.453LeuVal: 8.453 ± 0.084
1.259LeuTrp: 1.259 ± 0.026
1.873LeuTyr: 1.873 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
2.214MetAla: 2.214 ± 0.037
0.16MetCys: 0.16 ± 0.009
0.848MetAsp: 0.848 ± 0.023
0.734MetGlu: 0.734 ± 0.021
0.464MetPhe: 0.464 ± 0.017
1.308MetGly: 1.308 ± 0.032
0.345MetHis: 0.345 ± 0.013
0.657MetIle: 0.657 ± 0.019
0.409MetLys: 0.409 ± 0.014
1.585MetLeu: 1.585 ± 0.03
0.307MetMet: 0.307 ± 0.013
0.406MetAsn: 0.406 ± 0.016
1.102MetPro: 1.102 ± 0.024
0.441MetGln: 0.441 ± 0.015
1.408MetArg: 1.408 ± 0.031
1.294MetSer: 1.294 ± 0.027
1.467MetThr: 1.467 ± 0.029
1.226MetVal: 1.226 ± 0.029
0.211MetTrp: 0.211 ± 0.01
0.32MetTyr: 0.32 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.127AsnAla: 2.127 ± 0.034
0.158AsnCys: 0.158 ± 0.009
0.89AsnAsp: 0.89 ± 0.022
0.769AsnGlu: 0.769 ± 0.021
0.464AsnPhe: 0.464 ± 0.016
1.837AsnGly: 1.837 ± 0.037
0.377AsnHis: 0.377 ± 0.015
0.612AsnIle: 0.612 ± 0.017
0.416AsnLys: 0.416 ± 0.017
1.6AsnLeu: 1.6 ± 0.029
0.307AsnMet: 0.307 ± 0.013
0.379AsnAsn: 0.379 ± 0.016
1.284AsnPro: 1.284 ± 0.031
0.512AsnGln: 0.512 ± 0.019
1.245AsnArg: 1.245 ± 0.027
0.903AsnSer: 0.903 ± 0.025
1.04AsnThr: 1.04 ± 0.028
1.385AsnVal: 1.385 ± 0.028
0.274AsnTrp: 0.274 ± 0.011
0.382AsnTyr: 0.382 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
9.521ProAla: 9.521 ± 0.109
0.393ProCys: 0.393 ± 0.016
4.562ProAsp: 4.562 ± 0.053
4.271ProGlu: 4.271 ± 0.061
1.551ProPhe: 1.551 ± 0.029
7.21ProGly: 7.21 ± 0.079
1.542ProHis: 1.542 ± 0.029
1.238ProIle: 1.238 ± 0.023
1.223ProLys: 1.223 ± 0.026
5.333ProLeu: 5.333 ± 0.059
1.001ProMet: 1.001 ± 0.025
0.888ProAsn: 0.888 ± 0.023
3.956ProPro: 3.956 ± 0.067
1.762ProGln: 1.762 ± 0.032
4.732ProArg: 4.732 ± 0.072
3.209ProSer: 3.209 ± 0.049
3.163ProThr: 3.163 ± 0.045
5.487ProVal: 5.487 ± 0.054
0.854ProTrp: 0.854 ± 0.023
1.462ProTyr: 1.462 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.653GlnAla: 3.653 ± 0.051
0.174GlnCys: 0.174 ± 0.01
1.398GlnAsp: 1.398 ± 0.03
1.385GlnGlu: 1.385 ± 0.026
0.643GlnPhe: 0.643 ± 0.019
2.381GlnGly: 2.381 ± 0.038
0.677GlnHis: 0.677 ± 0.019
1.095GlnIle: 1.095 ± 0.027
0.664GlnLys: 0.664 ± 0.022
2.929GlnLeu: 2.929 ± 0.04
0.451GlnMet: 0.451 ± 0.016
0.517GlnAsn: 0.517 ± 0.017
1.687GlnPro: 1.687 ± 0.04
1.206GlnGln: 1.206 ± 0.032
2.396GlnArg: 2.396 ± 0.038
1.228GlnSer: 1.228 ± 0.024
1.375GlnThr: 1.375 ± 0.03
2.335GlnVal: 2.335 ± 0.036
0.477GlnTrp: 0.477 ± 0.016
0.617GlnTyr: 0.617 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
10.567ArgAla: 10.567 ± 0.097
0.698ArgCys: 0.698 ± 0.021
4.359ArgAsp: 4.359 ± 0.051
4.792ArgGlu: 4.792 ± 0.059
2.311ArgPhe: 2.311 ± 0.033
6.429ArgGly: 6.429 ± 0.069
2.353ArgHis: 2.353 ± 0.045
3.159ArgIle: 3.159 ± 0.046
1.599ArgLys: 1.599 ± 0.029
9.166ArgLeu: 9.166 ± 0.092
1.705ArgMet: 1.705 ± 0.03
1.305ArgAsn: 1.305 ± 0.028
6.183ArgPro: 6.183 ± 0.088
2.366ArgGln: 2.366 ± 0.032
9.262ArgArg: 9.262 ± 0.136
4.087ArgSer: 4.087 ± 0.052
5.795ArgThr: 5.795 ± 0.057
5.899ArgVal: 5.899 ± 0.058
1.368ArgTrp: 1.368 ± 0.027
1.792ArgTyr: 1.792 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.756SerAla: 6.756 ± 0.066
0.449SerCys: 0.449 ± 0.016
2.535SerAsp: 2.535 ± 0.04
2.236SerGlu: 2.236 ± 0.035
1.54SerPhe: 1.54 ± 0.032
6.007SerGly: 6.007 ± 0.073
0.969SerHis: 0.969 ± 0.022
1.326SerIle: 1.326 ± 0.024
0.965SerLys: 0.965 ± 0.026
4.581SerLeu: 4.581 ± 0.057
1.027SerMet: 1.027 ± 0.023
0.796SerAsn: 0.796 ± 0.022
3.299SerPro: 3.299 ± 0.05
1.174SerGln: 1.174 ± 0.027
3.654SerArg: 3.654 ± 0.043
2.764SerSer: 2.764 ± 0.056
2.947SerThr: 2.947 ± 0.045
4.164SerVal: 4.164 ± 0.044
0.831SerTrp: 0.831 ± 0.021
1.206SerTyr: 1.206 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
9.167ThrAla: 9.167 ± 0.083
0.452ThrCys: 0.452 ± 0.016
3.56ThrAsp: 3.56 ± 0.046
3.167ThrGlu: 3.167 ± 0.041
1.554ThrPhe: 1.554 ± 0.031
6.856ThrGly: 6.856 ± 0.062
1.197ThrHis: 1.197 ± 0.026
1.544ThrIle: 1.544 ± 0.031
1.148ThrLys: 1.148 ± 0.027
5.458ThrLeu: 5.458 ± 0.053
0.911ThrMet: 0.911 ± 0.022
0.915ThrAsn: 0.915 ± 0.024
4.174ThrPro: 4.174 ± 0.057
1.348ThrGln: 1.348 ± 0.027
4.065ThrArg: 4.065 ± 0.047
3.051ThrSer: 3.051 ± 0.048
3.685ThrThr: 3.685 ± 0.059
6.015ThrVal: 6.015 ± 0.058
0.849ThrTrp: 0.849 ± 0.023
1.324ThrTyr: 1.324 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
10.632ValAla: 10.632 ± 0.079
0.778ValCys: 0.778 ± 0.018
4.849ValAsp: 4.849 ± 0.048
4.502ValGlu: 4.502 ± 0.052
2.438ValPhe: 2.438 ± 0.035
6.337ValGly: 6.337 ± 0.071
2.027ValHis: 2.027 ± 0.026
2.748ValIle: 2.748 ± 0.043
1.808ValLys: 1.808 ± 0.037
9.311ValLeu: 9.311 ± 0.078
1.39ValMet: 1.39 ± 0.027
1.652ValAsn: 1.652 ± 0.031
5.402ValPro: 5.402 ± 0.06
2.03ValGln: 2.03 ± 0.032
7.492ValArg: 7.492 ± 0.067
4.158ValSer: 4.158 ± 0.047
5.632ValThr: 5.632 ± 0.054
7.884ValVal: 7.884 ± 0.078
1.106ValTrp: 1.106 ± 0.025
1.599ValTyr: 1.599 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.548TrpAla: 1.548 ± 0.028
0.161TrpCys: 0.161 ± 0.008
0.779TrpAsp: 0.779 ± 0.019
0.677TrpGlu: 0.677 ± 0.019
0.492TrpPhe: 0.492 ± 0.018
1.038TrpGly: 1.038 ± 0.023
0.358TrpHis: 0.358 ± 0.015
0.492TrpIle: 0.492 ± 0.015
0.369TrpLys: 0.369 ± 0.014
1.67TrpLeu: 1.67 ± 0.031
0.257TrpMet: 0.257 ± 0.012
0.383TrpAsn: 0.383 ± 0.017
0.798TrpPro: 0.798 ± 0.021
0.595TrpGln: 0.595 ± 0.02
1.325TrpArg: 1.325 ± 0.03
0.92TrpSer: 0.92 ± 0.022
1.081TrpThr: 1.081 ± 0.025
0.924TrpVal: 0.924 ± 0.023
0.32TrpTrp: 0.32 ± 0.012
0.341TrpTyr: 0.341 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.835TyrAla: 2.835 ± 0.039
0.18TyrCys: 0.18 ± 0.009
1.598TyrAsp: 1.598 ± 0.039
1.237TyrGlu: 1.237 ± 0.023
0.656TyrPhe: 0.656 ± 0.018
2.355TyrGly: 2.355 ± 0.031
0.39TyrHis: 0.39 ± 0.014
0.472TyrIle: 0.472 ± 0.015
0.419TyrLys: 0.419 ± 0.018
2.068TyrLeu: 2.068 ± 0.031
0.245TyrMet: 0.245 ± 0.012
0.403TyrAsn: 0.403 ± 0.016
1.06TyrPro: 1.06 ± 0.026
0.602TyrGln: 0.602 ± 0.02
1.792TyrArg: 1.792 ± 0.03
0.957TyrSer: 0.957 ± 0.024
1.204TyrThr: 1.204 ± 0.032
1.705TyrVal: 1.705 ± 0.026
0.348TyrTrp: 0.348 ± 0.014
0.463TyrTyr: 0.463 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6181 proteins (1971672 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski