Barley yellow mosaic virus (isolate China/Yancheng/1998) (BaYMV)
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9YJW3|POL1_BAYMY Genome polyprotein 1 OS=Barley yellow mosaic virus (isolate China/Yancheng/1998) OX=652104 PE=3 SV=1
MM1 pKa = 7.19 SASSSRR7 pKa = 11.84 LLFDD11 pKa = 5.05 CGSLDD16 pKa = 3.54 WPNKK20 pKa = 10.29 SLFGDD25 pKa = 3.61 PTTRR29 pKa = 11.84 DD30 pKa = 3.15 VMNEE34 pKa = 4.23 HH35 pKa = 7.39 ISSTWNAVIRR45 pKa = 11.84 RR46 pKa = 11.84 HH47 pKa = 5.13 MLAPNANAEE56 pKa = 4.47 TILGRR61 pKa = 11.84 DD62 pKa = 4.19 GLPSAQFDD70 pKa = 4.08 AYY72 pKa = 10.81 GAMLPSFIQALNAPTTRR89 pKa = 11.84 LRR91 pKa = 11.84 ISAPLSTAEE100 pKa = 5.41 SILCADD106 pKa = 4.6 ASHH109 pKa = 7.0 APWLYY114 pKa = 9.52 MANSVCAYY122 pKa = 7.72 EE123 pKa = 4.26 ATHH126 pKa = 6.24 LQPVQTFIAFNFAHH140 pKa = 7.17 GYY142 pKa = 10.07 CYY144 pKa = 10.69 LSLFIPLSFRR154 pKa = 11.84 ITFEE158 pKa = 3.64 NARR161 pKa = 11.84 GFSRR165 pKa = 11.84 FLEE168 pKa = 4.04 QLPDD172 pKa = 3.22 ILGAYY177 pKa = 6.29 PTLAAIYY184 pKa = 8.57 KK185 pKa = 9.31 TMLFAIRR192 pKa = 11.84 LFPEE196 pKa = 4.28 VLQAPIPIIAKK207 pKa = 10.06 RR208 pKa = 11.84 PGVLQFHH215 pKa = 6.5 VSDD218 pKa = 4.09 ARR220 pKa = 11.84 GLPPSWFPMKK230 pKa = 10.03 CGSVASFVALITNNLNSDD248 pKa = 4.46 LLNGIVGSNGDD259 pKa = 3.74 GEE261 pKa = 6.18 HH262 pKa = 5.02 YY263 pKa = 9.85 TNWNSGHH270 pKa = 6.16 DD271 pKa = 3.36 HH272 pKa = 6.69 WIVNRR277 pKa = 11.84 FITVKK282 pKa = 10.43 DD283 pKa = 3.4 LHH285 pKa = 7.07 SSLKK289 pKa = 10.47 SALEE293 pKa = 3.77 VDD295 pKa = 4.36 LDD297 pKa = 4.19 TEE299 pKa = 4.4 GGRR302 pKa = 11.84 NAVLDD307 pKa = 4.23 LLLDD311 pKa = 4.34 LGVTNLVRR319 pKa = 11.84 RR320 pKa = 11.84 EE321 pKa = 4.06 KK322 pKa = 10.63 RR323 pKa = 11.84 FPAYY327 pKa = 9.95 FQGAEE332 pKa = 4.14 SVYY335 pKa = 11.08 LLLSCEE341 pKa = 3.96 RR342 pKa = 11.84 VGNEE346 pKa = 3.6 LVAVQDD352 pKa = 4.09 ALQEE356 pKa = 4.04 PLANYY361 pKa = 10.2 SGLDD365 pKa = 3.47 LRR367 pKa = 11.84 ALIINLGGLPSRR379 pKa = 11.84 HH380 pKa = 6.18 SDD382 pKa = 2.34 ICYY385 pKa = 8.59 TRR387 pKa = 11.84 NIFEE391 pKa = 4.7 NDD393 pKa = 2.81 NHH395 pKa = 6.43 LVWNFEE401 pKa = 4.08 FYY403 pKa = 10.66 RR404 pKa = 11.84 IASITKK410 pKa = 8.76 NAQIDD415 pKa = 3.88 RR416 pKa = 11.84 DD417 pKa = 4.13 VLSSSMANLFSDD429 pKa = 5.03 FVSEE433 pKa = 4.46 SSNGQYY439 pKa = 9.58 RR440 pKa = 11.84 VKK442 pKa = 10.4 EE443 pKa = 4.01 PRR445 pKa = 11.84 PVVQYY450 pKa = 10.29 RR451 pKa = 11.84 VEE453 pKa = 4.02 HH454 pKa = 6.92 DD455 pKa = 3.7 EE456 pKa = 4.36 PVASSAPSAWWQVLIGITTAILGAIIFFLWRR487 pKa = 11.84 CFLRR491 pKa = 11.84 AKK493 pKa = 9.94 RR494 pKa = 11.84 VKK496 pKa = 10.21 FQAKK500 pKa = 10.25 DD501 pKa = 3.32 SFPWFTTSGDD511 pKa = 3.92 DD512 pKa = 5.29 DD513 pKa = 4.54 SPPPPGDD520 pKa = 3.6 SPSRR524 pKa = 11.84 PPGRR528 pKa = 11.84 SPDD531 pKa = 3.28 RR532 pKa = 11.84 VLPRR536 pKa = 11.84 TVVRR540 pKa = 11.84 DD541 pKa = 3.21 LSFNDD546 pKa = 4.63 DD547 pKa = 4.2 DD548 pKa = 5.27 DD549 pKa = 4.34 LHH551 pKa = 8.83 SVDD554 pKa = 5.0 LNEE557 pKa = 4.29 AGSRR561 pKa = 11.84 FGEE564 pKa = 4.5 VVSLIARR571 pKa = 11.84 GNLRR575 pKa = 11.84 EE576 pKa = 4.06 LAGAIPEE583 pKa = 4.27 SLSNLTLLQTSASGSGFYY601 pKa = 10.74 TMVALYY607 pKa = 10.28 LATLGDD613 pKa = 5.14 AITAFHH619 pKa = 6.46 EE620 pKa = 4.75 HH621 pKa = 6.49 NDD623 pKa = 3.46 ASPATIQSLRR633 pKa = 11.84 TLEE636 pKa = 4.28 LQLEE640 pKa = 4.31 ARR642 pKa = 11.84 GLRR645 pKa = 11.84 FNEE648 pKa = 4.16 AGTPANLIQRR658 pKa = 11.84 GVNSSVGRR666 pKa = 11.84 ALVRR670 pKa = 11.84 LTQSALLATGEE681 pKa = 4.06 NFRR684 pKa = 11.84 TRR686 pKa = 11.84 MATTLEE692 pKa = 4.32 RR693 pKa = 11.84 IAAEE697 pKa = 3.98 RR698 pKa = 11.84 LNTLTAYY705 pKa = 9.05 DD706 pKa = 3.44 QRR708 pKa = 11.84 VIEE711 pKa = 4.33 MTTEE715 pKa = 3.9 LLAAIKK721 pKa = 9.93 PVLEE725 pKa = 4.27 VEE727 pKa = 4.21 RR728 pKa = 11.84 SEE730 pKa = 4.46 LTPHH734 pKa = 7.39 LANAEE739 pKa = 4.09 ALLQVYY745 pKa = 10.56 NNLFSTDD752 pKa = 3.72 YY753 pKa = 11.28 VSASLLALRR762 pKa = 11.84 RR763 pKa = 11.84 EE764 pKa = 4.29 MILRR768 pKa = 11.84 SAEE771 pKa = 3.85 GRR773 pKa = 11.84 VGEE776 pKa = 4.38 QPTSASDD783 pKa = 3.27 AANEE787 pKa = 4.01 EE788 pKa = 4.31 LVQRR792 pKa = 11.84 SMTKK796 pKa = 9.78 LDD798 pKa = 3.6 KK799 pKa = 10.87 EE800 pKa = 4.15 IEE802 pKa = 4.13 LFQAQIDD809 pKa = 4.05 SQRR812 pKa = 11.84 RR813 pKa = 11.84 AVTITEE819 pKa = 4.0 ASNLRR824 pKa = 11.84 EE825 pKa = 4.86 NILQPINTVANIAMAGAFLRR845 pKa = 11.84 GGARR849 pKa = 11.84 HH850 pKa = 6.37 RR851 pKa = 11.84 MPGMPDD857 pKa = 2.87 VATPMPNPFRR867 pKa = 11.84 AFSGRR872 pKa = 11.84 GHH874 pKa = 6.64 SLTTTRR880 pKa = 11.84 SGGLFRR886 pKa = 11.84 RR887 pKa = 11.84 PRR889 pKa = 11.84 VV890 pKa = 3.29
Molecular weight: 98.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.837
IPC2_protein 5.741
IPC_protein 5.792
Toseland 6.211
ProMoST 6.148
Dawson 6.059
Bjellqvist 6.071
Wikipedia 6.059
Rodwell 6.033
Grimsley 6.376
Solomon 6.059
Lehninger 6.046
Nozaki 6.313
DTASelect 6.504
Thurlkill 6.517
EMBOSS 6.478
Sillero 6.415
Patrickios 4.355
IPC_peptide 6.071
IPC2_peptide 6.427
IPC2.peptide.svr19 6.334
Protein with the highest isoelectric point:
>sp|Q9YJW3|POL1_BAYMY Genome polyprotein 1 OS=Barley yellow mosaic virus (isolate China/Yancheng/1998) OX=652104 PE=3 SV=1
MM1 pKa = 7.32 EE2 pKa = 3.97 QTLAQAVSRR11 pKa = 11.84 RR12 pKa = 11.84 NKK14 pKa = 9.18 TDD16 pKa = 2.71 TPMAEE21 pKa = 4.2 EE22 pKa = 4.42 RR23 pKa = 11.84 KK24 pKa = 9.93 HH25 pKa = 5.3 FSPMNFSANFVAPEE39 pKa = 4.26 LFYY42 pKa = 11.05 SANVRR47 pKa = 11.84 KK48 pKa = 9.75 IKK50 pKa = 10.77 NIFKK54 pKa = 10.18 EE55 pKa = 4.14 RR56 pKa = 11.84 STTRR60 pKa = 11.84 FLDD63 pKa = 4.6 AISSDD68 pKa = 3.79 FEE70 pKa = 4.2 LVAFLTLSPAHH81 pKa = 6.7 LMQLEE86 pKa = 4.4 TVLRR90 pKa = 11.84 QEE92 pKa = 4.38 MRR94 pKa = 11.84 SCAVPIVTSDD104 pKa = 3.01 ASFEE108 pKa = 4.21 TVAVIKK114 pKa = 9.45 TALDD118 pKa = 3.11 GMRR121 pKa = 11.84 FHH123 pKa = 7.62 FGYY126 pKa = 7.27 TTLEE130 pKa = 4.19 KK131 pKa = 10.76 GWISMMRR138 pKa = 11.84 HH139 pKa = 5.98 AEE141 pKa = 3.92 SCLQEE146 pKa = 4.33 SSSSAVNDD154 pKa = 3.29 LQTPIKK160 pKa = 10.36 RR161 pKa = 11.84 VGSLLLSGKK170 pKa = 9.68 NRR172 pKa = 11.84 VEE174 pKa = 3.98 GCEE177 pKa = 4.02 LSVLNLTARR186 pKa = 11.84 RR187 pKa = 11.84 FRR189 pKa = 11.84 IEE191 pKa = 3.66 YY192 pKa = 9.45 GLNGTYY198 pKa = 10.22 FGEE201 pKa = 4.35 HH202 pKa = 4.79 VAMLRR207 pKa = 11.84 DD208 pKa = 4.19 LKK210 pKa = 10.72 RR211 pKa = 11.84 YY212 pKa = 9.74 IYY214 pKa = 9.65 GTVPKK219 pKa = 9.8 EE220 pKa = 3.8 FLWAKK225 pKa = 7.51 TKK227 pKa = 10.5 KK228 pKa = 9.46 HH229 pKa = 6.07 LPFTIPAWITRR240 pKa = 11.84 TPIDD244 pKa = 3.96 CFLFCLRR251 pKa = 11.84 VIPILHH257 pKa = 6.54 RR258 pKa = 11.84 FGVAMSLIYY267 pKa = 9.03 FSCVAALNFPAFMGFLFKK285 pKa = 10.74 RR286 pKa = 11.84 QFAKK290 pKa = 10.91 YY291 pKa = 8.92 LAHH294 pKa = 6.9 SFAKK298 pKa = 10.24 HH299 pKa = 5.54 SIYY302 pKa = 10.73 FMFLTIVAILWSFRR316 pKa = 11.84 TFTSQKK322 pKa = 10.1 PKK324 pKa = 10.24 IVLQARR330 pKa = 11.84 STAEE334 pKa = 3.96 KK335 pKa = 9.94 EE336 pKa = 4.34 KK337 pKa = 10.83 KK338 pKa = 10.63 LMMILASVVGITYY351 pKa = 10.58 LFDD354 pKa = 3.74 YY355 pKa = 10.72 DD356 pKa = 3.3 IAEE359 pKa = 4.21 ALGNCLHH366 pKa = 6.79 KK367 pKa = 10.41 ISRR370 pKa = 11.84 LSSYY374 pKa = 11.03 LLDD377 pKa = 3.83 DD378 pKa = 4.04 HH379 pKa = 7.58 QGIASRR385 pKa = 11.84 MFGASYY391 pKa = 10.7 GLQAGDD397 pKa = 3.64 SAEE400 pKa = 4.26 DD401 pKa = 3.38 AVTTIISDD409 pKa = 4.07 LLSVTFKK416 pKa = 10.79 IVDD419 pKa = 3.44 EE420 pKa = 4.86 DD421 pKa = 4.03 ASQGTVEE428 pKa = 4.93 DD429 pKa = 3.99 ASEE432 pKa = 4.34 TTFHH436 pKa = 6.2 SWVGVNTLAGRR447 pKa = 11.84 NMSRR451 pKa = 11.84 PLQYY455 pKa = 10.85 SVNKK459 pKa = 9.49 TYY461 pKa = 11.34 ALTPQNVQLQARR473 pKa = 11.84 AMADD477 pKa = 3.66 ANNCWSMVVGHH488 pKa = 6.61 TGSGKK493 pKa = 8.36 STYY496 pKa = 10.91 LPVQYY501 pKa = 11.06 SNYY504 pKa = 10.26 LSTKK508 pKa = 8.59 SDD510 pKa = 3.0 RR511 pKa = 11.84 RR512 pKa = 11.84 QQILICEE519 pKa = 4.34 PTQAATEE526 pKa = 4.14 NVCAGVAANLGRR538 pKa = 11.84 AVYY541 pKa = 9.78 GRR543 pKa = 11.84 HH544 pKa = 5.73 EE545 pKa = 4.3 GWSRR549 pKa = 11.84 MGDD552 pKa = 3.13 HH553 pKa = 7.88 CIQVMTYY560 pKa = 10.39 GSALQCHH567 pKa = 6.11 AMDD570 pKa = 4.85 PSFISTFDD578 pKa = 4.3 AIFLDD583 pKa = 4.05 EE584 pKa = 4.08 AHH586 pKa = 6.82 DD587 pKa = 4.1 VKK589 pKa = 10.96 EE590 pKa = 3.89 HH591 pKa = 5.98 SLVFEE596 pKa = 4.58 SICDD600 pKa = 3.51 TFKK603 pKa = 10.9 SVRR606 pKa = 11.84 KK607 pKa = 8.97 FYY609 pKa = 11.34 VSATPRR615 pKa = 11.84 DD616 pKa = 3.67 GSVCPEE622 pKa = 3.95 AARR625 pKa = 11.84 KK626 pKa = 9.43 YY627 pKa = 8.11 PLHH630 pKa = 7.25 VEE632 pKa = 4.06 TSVCDD637 pKa = 3.57 SYY639 pKa = 11.88 RR640 pKa = 11.84 KK641 pKa = 10.04 FIAAQGGGDD650 pKa = 3.87 LLDD653 pKa = 3.7 ISKK656 pKa = 10.6 HH657 pKa = 4.61 DD658 pKa = 3.51 TALVFLAGRR667 pKa = 11.84 PEE669 pKa = 4.53 CIKK672 pKa = 10.79 AANAWNASVTGEE684 pKa = 3.85 KK685 pKa = 10.08 RR686 pKa = 11.84 AFSLSSDD693 pKa = 3.21 NFATDD698 pKa = 3.54 FSMLTEE704 pKa = 3.85 RR705 pKa = 11.84 LKK707 pKa = 9.1 THH709 pKa = 5.52 KK710 pKa = 9.63 TIIFTTNIIEE720 pKa = 4.39 TGVTLSVDD728 pKa = 3.58 CVVDD732 pKa = 4.87 FGHH735 pKa = 6.59 TMRR738 pKa = 11.84 PCLDD742 pKa = 4.05 LNQKK746 pKa = 7.67 SLRR749 pKa = 11.84 LDD751 pKa = 3.19 RR752 pKa = 11.84 RR753 pKa = 11.84 KK754 pKa = 8.86 VTRR757 pKa = 11.84 NEE759 pKa = 3.61 RR760 pKa = 11.84 QQRR763 pKa = 11.84 IGPTGRR769 pKa = 11.84 LKK771 pKa = 10.78 DD772 pKa = 3.91 GYY774 pKa = 11.04 AIVCGDD780 pKa = 3.19 VDD782 pKa = 3.57 RR783 pKa = 11.84 AVNVISPDD791 pKa = 3.45 VLYY794 pKa = 10.79 GAALLSFKK802 pKa = 10.92 HH803 pKa = 5.77 NVPFYY808 pKa = 9.87 MNEE811 pKa = 3.94 TFEE814 pKa = 4.66 SSWLKK819 pKa = 10.95 GVTKK823 pKa = 10.57 AQADD827 pKa = 3.63 TMTIFKK833 pKa = 10.75 LPIFLTRR840 pKa = 11.84 DD841 pKa = 4.22 LINADD846 pKa = 3.05 GSVAKK851 pKa = 10.55 EE852 pKa = 3.71 FLDD855 pKa = 3.77 VLKK858 pKa = 10.56 KK859 pKa = 10.22 HH860 pKa = 5.94 QFTTSDD866 pKa = 2.83 IKK868 pKa = 10.64 QAPSITAKK876 pKa = 10.55 HH877 pKa = 6.06 IFPTWASYY885 pKa = 10.64 FSLHH889 pKa = 5.95 QALHH893 pKa = 5.97 YY894 pKa = 11.09 GDD896 pKa = 5.49 DD897 pKa = 3.75 KK898 pKa = 11.98 DD899 pKa = 4.74 EE900 pKa = 4.6 IPHH903 pKa = 5.48 EE904 pKa = 3.96 LRR906 pKa = 11.84 YY907 pKa = 10.37 ARR909 pKa = 11.84 VPFSVTTLSKK919 pKa = 10.68 FDD921 pKa = 3.6 WPALALACEE930 pKa = 4.27 KK931 pKa = 10.61 HH932 pKa = 5.91 RR933 pKa = 11.84 ASMSNVFAGIEE944 pKa = 3.88 EE945 pKa = 4.28 PARR948 pKa = 11.84 VVTLQTNPANIQASITHH965 pKa = 6.75 LMHH968 pKa = 6.34 MSKK971 pKa = 10.47 NYY973 pKa = 7.8 KK974 pKa = 8.49 TLIEE978 pKa = 4.37 NNQHH982 pKa = 5.04 VRR984 pKa = 11.84 QSMMTNVMYY993 pKa = 10.3 KK994 pKa = 8.9 WFSSTRR1000 pKa = 11.84 ITKK1003 pKa = 10.43 DD1004 pKa = 2.48 LDD1006 pKa = 3.68 RR1007 pKa = 11.84 NLRR1010 pKa = 11.84 RR1011 pKa = 11.84 CTDD1014 pKa = 3.08 NLAVVEE1020 pKa = 4.37 ATLSSLRR1027 pKa = 11.84 QILAGNTQVHH1037 pKa = 6.28 ATPHH1041 pKa = 4.96 MQSTLEE1047 pKa = 4.8 DD1048 pKa = 3.8 IIGLQASDD1056 pKa = 3.55 TLTEE1060 pKa = 4.02 EE1061 pKa = 4.44 SLASALGIFVPKK1073 pKa = 10.7 SNLFLLLATKK1083 pKa = 10.15 GFKK1086 pKa = 9.94 LVYY1089 pKa = 9.22 VICILLLVNLVYY1101 pKa = 10.55 IGLRR1105 pKa = 11.84 KK1106 pKa = 9.02 WRR1108 pKa = 11.84 EE1109 pKa = 3.51 HH1110 pKa = 6.1 LKK1112 pKa = 10.66 QKK1114 pKa = 10.84 GSDD1117 pKa = 3.72 EE1118 pKa = 4.18 ILTNTMPVSEE1128 pKa = 4.6 GGEE1131 pKa = 3.73 ILAEE1135 pKa = 4.12 VMKK1138 pKa = 10.04 MEE1140 pKa = 4.54 PKK1142 pKa = 9.0 MRR1144 pKa = 11.84 KK1145 pKa = 9.09 NIKK1148 pKa = 9.66 RR1149 pKa = 11.84 DD1150 pKa = 2.86 MDD1152 pKa = 3.69 EE1153 pKa = 4.07 AVEE1156 pKa = 4.3 SKK1158 pKa = 10.99 LCGFTFVFPDD1168 pKa = 3.62 DD1169 pKa = 4.73 DD1170 pKa = 5.41 KK1171 pKa = 11.64 IGLEE1175 pKa = 4.26 GKK1177 pKa = 9.38 GNKK1180 pKa = 8.85 YY1181 pKa = 10.14 RR1182 pKa = 11.84 PRR1184 pKa = 11.84 EE1185 pKa = 4.08 DD1186 pKa = 3.16 ARR1188 pKa = 11.84 LMYY1191 pKa = 9.35 STRR1194 pKa = 11.84 EE1195 pKa = 4.04 DD1196 pKa = 3.28 ATFDD1200 pKa = 3.09 AWNEE1204 pKa = 3.83 KK1205 pKa = 10.33 AKK1207 pKa = 10.3 EE1208 pKa = 3.94 RR1209 pKa = 11.84 RR1210 pKa = 11.84 KK1211 pKa = 10.39 KK1212 pKa = 9.65 VTDD1215 pKa = 3.24 KK1216 pKa = 11.4 SEE1218 pKa = 4.21 PEE1220 pKa = 3.54 LRR1222 pKa = 11.84 RR1223 pKa = 11.84 AYY1225 pKa = 9.92 EE1226 pKa = 3.58 KK1227 pKa = 10.44 RR1228 pKa = 11.84 PYY1230 pKa = 10.7 FNFYY1234 pKa = 11.02 DD1235 pKa = 3.92 LQTDD1239 pKa = 3.9 SNILEE1244 pKa = 4.63 AIFYY1248 pKa = 6.42 TTEE1251 pKa = 3.61 GDD1253 pKa = 3.74 EE1254 pKa = 4.67 FFRR1257 pKa = 11.84 TADD1260 pKa = 3.55 PNKK1263 pKa = 10.7 DD1264 pKa = 3.4 MNLVADD1270 pKa = 4.5 KK1271 pKa = 10.88 LRR1273 pKa = 11.84 SFLDD1277 pKa = 3.29 TKK1279 pKa = 10.9 LVVGHH1284 pKa = 6.13 HH1285 pKa = 4.63 QRR1287 pKa = 11.84 KK1288 pKa = 8.9 LLEE1291 pKa = 3.79 EE1292 pKa = 4.04 TAQVVIKK1299 pKa = 9.11 DD1300 pKa = 4.01 TKK1302 pKa = 8.78 GTAHH1306 pKa = 7.09 KK1307 pKa = 10.22 MEE1309 pKa = 5.06 ISQHH1313 pKa = 6.88 DD1314 pKa = 4.37 PDD1316 pKa = 4.61 CLKK1319 pKa = 10.94 QNGSGKK1325 pKa = 10.15 VGYY1328 pKa = 8.92 PEE1330 pKa = 4.61 HH1331 pKa = 7.21 RR1332 pKa = 11.84 GQFRR1336 pKa = 11.84 QEE1338 pKa = 3.7 GVAITSDD1345 pKa = 3.3 YY1346 pKa = 11.55 DD1347 pKa = 3.63 LGVEE1351 pKa = 5.4 FGTDD1355 pKa = 3.0 TDD1357 pKa = 4.44 NITLEE1362 pKa = 4.16 ASTGILLSQVGVDD1375 pKa = 3.06 VATRR1379 pKa = 11.84 VGRR1382 pKa = 11.84 ISIGTFNMNCYY1393 pKa = 10.09 FYY1395 pKa = 11.02 NDD1397 pKa = 4.05 WILVPGHH1404 pKa = 6.16 LQDD1407 pKa = 3.74 RR1408 pKa = 11.84 SGNVTIQFPDD1418 pKa = 3.22 QTVQTTTDD1426 pKa = 3.15 ALNANGVKK1434 pKa = 10.06 RR1435 pKa = 11.84 FYY1437 pKa = 10.99 GLDD1440 pKa = 3.3 VIAIRR1445 pKa = 11.84 RR1446 pKa = 11.84 PAILRR1451 pKa = 11.84 PRR1453 pKa = 11.84 TKK1455 pKa = 10.18 LVKK1458 pKa = 10.39 AFAIEE1463 pKa = 4.19 EE1464 pKa = 4.09 PVIAQMVFVDD1474 pKa = 3.77 AQGVRR1479 pKa = 11.84 KK1480 pKa = 8.31 FTQSDD1485 pKa = 3.53 WARR1488 pKa = 11.84 KK1489 pKa = 9.29 GEE1491 pKa = 3.91 NSGRR1495 pKa = 11.84 WSHH1498 pKa = 7.05 KK1499 pKa = 8.99 ISTVLGMCGCQFWTLEE1515 pKa = 3.93 RR1516 pKa = 11.84 QIDD1519 pKa = 4.72 GIHH1522 pKa = 5.99 VATNYY1527 pKa = 7.05 TKK1529 pKa = 10.55 KK1530 pKa = 10.51 RR1531 pKa = 11.84 NEE1533 pKa = 3.98 FQPFTQEE1540 pKa = 3.65 VVDD1543 pKa = 5.5 FINGPGTKK1551 pKa = 8.89 IPYY1554 pKa = 9.1 CPWVFDD1560 pKa = 4.85 RR1561 pKa = 11.84 PACGYY1566 pKa = 10.51 ASHH1569 pKa = 6.84 TALFEE1574 pKa = 4.39 KK1575 pKa = 9.34 PTTLTDD1581 pKa = 4.02 VIHH1584 pKa = 5.83 MQASDD1589 pKa = 3.56 GLHH1592 pKa = 6.51 NINNAIEE1599 pKa = 4.5 GFGSSLRR1606 pKa = 11.84 GQLVSPPTEE1615 pKa = 3.92 STRR1618 pKa = 11.84 QRR1620 pKa = 11.84 FDD1622 pKa = 3.04 KK1623 pKa = 11.11 LFGSGSFEE1631 pKa = 6.52 LIGQMNKK1638 pKa = 10.4 GLIDD1642 pKa = 3.09 KK1643 pKa = 10.49 HH1644 pKa = 7.35 VIVGEE1649 pKa = 4.0 NDD1651 pKa = 3.16 DD1652 pKa = 4.57 VYY1654 pKa = 11.62 DD1655 pKa = 5.13 FMRR1658 pKa = 11.84 EE1659 pKa = 4.0 HH1660 pKa = 7.05 PTFTWLKK1667 pKa = 11.03 DD1668 pKa = 3.43 FMNEE1672 pKa = 3.79 YY1673 pKa = 10.43 APSVLSYY1680 pKa = 10.19 SAYY1683 pKa = 10.91 YY1684 pKa = 10.37 KK1685 pKa = 10.33 DD1686 pKa = 3.08 LCKK1689 pKa = 10.69 YY1690 pKa = 10.13 NRR1692 pKa = 11.84 AKK1694 pKa = 10.54 HH1695 pKa = 5.11 VLTYY1699 pKa = 10.77 NPEE1702 pKa = 3.95 EE1703 pKa = 3.61 LHH1705 pKa = 6.29 YY1706 pKa = 9.74 ATKK1709 pKa = 10.92 GLIKK1713 pKa = 9.87 MLEE1716 pKa = 4.26 DD1717 pKa = 3.89 AGLTQGSVRR1726 pKa = 11.84 TPQQVISDD1734 pKa = 4.08 IQWNTSAGPSYY1745 pKa = 10.21 QGKK1748 pKa = 9.58 KK1749 pKa = 9.72 RR1750 pKa = 11.84 DD1751 pKa = 3.79 LCAHH1755 pKa = 6.87 LSDD1758 pKa = 6.16 DD1759 pKa = 4.33 EE1760 pKa = 4.4 VLHH1763 pKa = 5.96 LAEE1766 pKa = 4.17 VCRR1769 pKa = 11.84 QQFLEE1774 pKa = 4.56 GKK1776 pKa = 8.25 STGVWNGSLKK1786 pKa = 11.0 AEE1788 pKa = 4.08 LRR1790 pKa = 11.84 TIEE1793 pKa = 4.19 KK1794 pKa = 10.41 VEE1796 pKa = 3.97 PEE1798 pKa = 3.74 KK1799 pKa = 10.69 TRR1801 pKa = 11.84 VFTASPITSLFAMKK1815 pKa = 9.96 FYY1817 pKa = 11.29 VDD1819 pKa = 4.25 DD1820 pKa = 4.26 FNKK1823 pKa = 10.2 KK1824 pKa = 10.02 FYY1826 pKa = 9.88 ATNLKK1831 pKa = 10.28 APHH1834 pKa = 5.85 TVGINKK1840 pKa = 9.78 FGRR1843 pKa = 11.84 GWEE1846 pKa = 4.01 RR1847 pKa = 11.84 LHH1849 pKa = 7.54 DD1850 pKa = 4.06 KK1851 pKa = 10.74 LNRR1854 pKa = 11.84 PGWLHH1859 pKa = 6.24 GSGDD1863 pKa = 3.57 GSRR1866 pKa = 11.84 FDD1868 pKa = 4.47 SSIDD1872 pKa = 3.3 PFFFDD1877 pKa = 3.39 VVKK1880 pKa = 10.01 TIRR1883 pKa = 11.84 KK1884 pKa = 8.29 HH1885 pKa = 5.02 FLPSEE1890 pKa = 3.85 HH1891 pKa = 6.85 HH1892 pKa = 6.14 KK1893 pKa = 11.01 AIDD1896 pKa = 4.42 LIYY1899 pKa = 10.91 DD1900 pKa = 4.48 EE1901 pKa = 5.16 ILNTTICLANGMVIKK1916 pKa = 10.7 KK1917 pKa = 10.07 NVGTQRR1923 pKa = 11.84 QPSTVVDD1930 pKa = 3.53 NTLVLMTAFLYY1941 pKa = 10.42 AYY1943 pKa = 9.04 IHH1945 pKa = 5.68 KK1946 pKa = 9.47 TGDD1949 pKa = 3.28 RR1950 pKa = 11.84 EE1951 pKa = 4.01 LALLNEE1957 pKa = 4.48 RR1958 pKa = 11.84 FIFVCNGDD1966 pKa = 3.89 DD1967 pKa = 3.49 NKK1969 pKa = 10.65 FAISPQFDD1977 pKa = 3.55 EE1978 pKa = 5.22 EE1979 pKa = 4.37 FGHH1982 pKa = 7.01 DD1983 pKa = 4.24 FSPEE1987 pKa = 3.88 LVEE1990 pKa = 6.11 LGLTYY1995 pKa = 10.64 EE1996 pKa = 4.61 FDD1998 pKa = 6.18 DD1999 pKa = 3.82 ITSDD2003 pKa = 3.15 ICEE2006 pKa = 4.25 NPYY2009 pKa = 10.23 MSLTMVKK2016 pKa = 9.01 TPFGVGFSLPVEE2028 pKa = 4.41 RR2029 pKa = 11.84 IIAIMQWSKK2038 pKa = 11.29 KK2039 pKa = 10.2 GGVLHH2044 pKa = 7.07 SYY2046 pKa = 10.24 LAGISAIYY2054 pKa = 10.31 EE2055 pKa = 4.22 SFNTPKK2061 pKa = 10.44 LFKK2064 pKa = 10.56 SIYY2067 pKa = 10.34 AYY2069 pKa = 10.67 LLWLTEE2075 pKa = 3.85 EE2076 pKa = 4.56 HH2077 pKa = 6.32 EE2078 pKa = 4.4 AEE2080 pKa = 4.12 ILAAMTQSSTALPIPSMLDD2099 pKa = 3.08 VYY2101 pKa = 10.54 RR2102 pKa = 11.84 LHH2104 pKa = 7.86 YY2105 pKa = 10.78 GDD2107 pKa = 5.64 DD2108 pKa = 4.39 EE2109 pKa = 4.4 IWLQAADD2116 pKa = 4.62 PLTDD2120 pKa = 3.54 AQKK2123 pKa = 10.65 EE2124 pKa = 4.19 AAHH2127 pKa = 5.91 TAAADD2132 pKa = 4.06 RR2133 pKa = 11.84 ARR2135 pKa = 11.84 LDD2137 pKa = 3.64 LADD2140 pKa = 3.92 ADD2142 pKa = 3.81 RR2143 pKa = 11.84 RR2144 pKa = 11.84 RR2145 pKa = 11.84 KK2146 pKa = 10.0 VEE2148 pKa = 3.64 ADD2150 pKa = 3.2 RR2151 pKa = 11.84 VEE2153 pKa = 3.87 AARR2156 pKa = 11.84 VKK2158 pKa = 10.33 KK2159 pKa = 10.51 AADD2162 pKa = 3.36 AALKK2166 pKa = 9.81 PVNLTATRR2174 pKa = 11.84 MPTEE2178 pKa = 5.04 DD2179 pKa = 4.01 DD2180 pKa = 4.14 GKK2182 pKa = 11.29 LKK2184 pKa = 9.91 TPSGARR2190 pKa = 11.84 IPSSAADD2197 pKa = 4.14 GNWSVPATKK2206 pKa = 9.93 QVNAGLTLKK2215 pKa = 10.58 IPLNKK2220 pKa = 9.96 LKK2222 pKa = 10.58 SVPKK2226 pKa = 10.4 SVMEE2230 pKa = 4.47 HH2231 pKa = 5.85 NNSVALEE2238 pKa = 4.29 SEE2240 pKa = 4.3 LKK2242 pKa = 10.48 AWTDD2246 pKa = 3.49 AVRR2249 pKa = 11.84 TSLGITTDD2257 pKa = 3.72 EE2258 pKa = 3.89 AWIDD2262 pKa = 3.44 ALIPFIGWCCNNGTSDD2278 pKa = 3.33 KK2279 pKa = 10.76 HH2280 pKa = 8.31 AEE2282 pKa = 4.07 NQVMQIDD2289 pKa = 4.02 SGKK2292 pKa = 10.31 GAVTEE2297 pKa = 4.36 MSLSPFIVHH2306 pKa = 6.61 ARR2308 pKa = 11.84 MNGGLRR2314 pKa = 11.84 RR2315 pKa = 11.84 IMRR2318 pKa = 11.84 NYY2320 pKa = 10.35 SDD2322 pKa = 3.7 EE2323 pKa = 4.34 TVLLITNNKK2332 pKa = 8.52 LVAHH2336 pKa = 7.45 WSMKK2340 pKa = 10.27 HH2341 pKa = 5.24 GASANAKK2348 pKa = 8.29 YY2349 pKa = 10.64 AFDD2352 pKa = 4.05 FFVPRR2357 pKa = 11.84 SWMNPQDD2364 pKa = 3.63 IEE2366 pKa = 4.24 VSKK2369 pKa = 10.26 QARR2372 pKa = 11.84 LAALGTGTYY2381 pKa = 8.83 NTMLTSDD2388 pKa = 3.71 TTNLRR2393 pKa = 11.84 KK2394 pKa = 7.11 TTNHH2398 pKa = 6.47 RR2399 pKa = 11.84 VLDD2402 pKa = 4.1 SDD2404 pKa = 3.79 GHH2406 pKa = 6.39 PEE2408 pKa = 3.88 LTT2410 pKa = 4.08
Molecular weight: 270.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.659
IPC2_protein 6.854
IPC_protein 6.795
Toseland 6.839
ProMoST 7.322
Dawson 7.351
Bjellqvist 7.571
Wikipedia 7.322
Rodwell 7.366
Grimsley 6.971
Solomon 7.366
Lehninger 7.38
Nozaki 7.834
DTASelect 7.644
Thurlkill 7.702
EMBOSS 7.688
Sillero 7.834
Patrickios 4.393
IPC_peptide 7.366
IPC2_peptide 7.132
IPC2.peptide.svr19 6.933
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3300
890
2410
1650.0
184.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.606 ± 0.721
1.364 ± 0.222
5.818 ± 0.365
5.364 ± 0.04
5.03 ± 0.095
5.727 ± 0.055
2.727 ± 0.283
5.364 ± 0.093
4.939 ± 1.557
9.727 ± 0.99
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.485 ± 0.275
4.576 ± 0.391
4.242 ± 0.604
3.303 ± 0.075
5.97 ± 0.746
7.424 ± 0.587
6.97 ± 0.539
6.242 ± 0.245
1.333 ± 0.047
2.788 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here