Lactococcus phage BM13
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9QMA2|R9QMA2_9CAUD Uncharacterized protein OS=Lactococcus phage BM13 OX=1229751 GN=BM13_33 PE=4 SV=1
MM1 pKa = 7.35 TDD3 pKa = 3.24 KK4 pKa = 11.17 LISLVNDD11 pKa = 3.12 WRR13 pKa = 11.84 GGFYY17 pKa = 10.77 LMPEE21 pKa = 4.1 DD22 pKa = 4.04 TPEE25 pKa = 3.89 EE26 pKa = 3.99 EE27 pKa = 4.03 LRR29 pKa = 11.84 EE30 pKa = 4.04 VEE32 pKa = 5.18 VYY34 pKa = 10.96 CDD36 pKa = 3.23 TCGDD40 pKa = 3.54 NDD42 pKa = 4.7 SIIGQFSNWNQLKK55 pKa = 10.45 KK56 pKa = 11.08 EE57 pKa = 4.06 MTDD60 pKa = 3.5 DD61 pKa = 4.56 EE62 pKa = 5.2 GWCPYY67 pKa = 10.28 SDD69 pKa = 5.14 EE70 pKa = 4.2 YY71 pKa = 10.46 LQSVFEE77 pKa = 4.85 EE78 pKa = 4.86 DD79 pKa = 3.67 DD80 pKa = 3.23 QQ81 pKa = 6.2
Molecular weight: 9.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.63
IPC_protein 3.579
Toseland 3.389
ProMoST 3.757
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.516
Rodwell 3.427
Grimsley 3.3
Solomon 3.554
Lehninger 3.503
Nozaki 3.706
DTASelect 3.884
Thurlkill 3.452
EMBOSS 3.516
Sillero 3.706
Patrickios 0.769
IPC_peptide 3.554
IPC2_peptide 3.681
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|R9QMT7|R9QMT7_9CAUD Putative anti-repressor OS=Lactococcus phage BM13 OX=1229751 GN=BM13_02 PE=4 SV=1
MM1 pKa = 7.72 LFQIQIQITKK11 pKa = 10.36 CFGRR15 pKa = 11.84 DD16 pKa = 2.96 KK17 pKa = 11.44 APFSKK22 pKa = 10.51 ARR24 pKa = 11.84 ALFLCYY30 pKa = 10.22 NIVGMFVRR38 pKa = 11.84 FHH40 pKa = 7.12 PIPRR44 pKa = 11.84 VKK46 pKa = 10.41 PFFGVAFFVCCKK58 pKa = 8.66 FTTILSEE65 pKa = 4.18 TT66 pKa = 3.81
Molecular weight: 7.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.132
IPC2_protein 9.063
IPC_protein 9.092
Toseland 9.999
ProMoST 9.575
Dawson 10.131
Bjellqvist 9.853
Wikipedia 10.233
Rodwell 10.687
Grimsley 10.145
Solomon 10.204
Lehninger 10.189
Nozaki 10.175
DTASelect 9.765
Thurlkill 10.028
EMBOSS 10.379
Sillero 10.101
Patrickios 10.599
IPC_peptide 10.204
IPC2_peptide 9.063
IPC2.peptide.svr19 7.901
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
9662
44
733
175.7
19.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.531 ± 0.649
0.486 ± 0.093
6.2 ± 0.411
7.235 ± 0.625
4.782 ± 0.279
6.645 ± 0.58
1.283 ± 0.166
7.162 ± 0.346
9.025 ± 0.533
7.617 ± 0.38
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.339 ± 0.155
6.52 ± 0.37
2.66 ± 0.179
4.73 ± 0.384
3.767 ± 0.293
5.961 ± 0.275
6.034 ± 0.393
5.951 ± 0.274
1.19 ± 0.123
3.881 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here