Amino acid dipepetide frequency for Clostridium aceticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.365AlaAla: 5.365 ± 0.098	0.69AlaCys: 0.69 ± 0.03	2.824AlaAsp: 2.824 ± 0.058	4.341AlaGlu: 4.341 ± 0.07	2.865AlaPhe: 2.865 ± 0.049	4.674AlaGly: 4.674 ± 0.08	0.967AlaHis: 0.967 ± 0.03	6.22AlaIle: 6.22 ± 0.072	4.581AlaLys: 4.581 ± 0.071	6.942AlaLeu: 6.942 ± 0.084	2.055AlaMet: 2.055 ± 0.043	2.606AlaAsn: 2.606 ± 0.055	1.759AlaPro: 1.759 ± 0.039	1.905AlaGln: 1.905 ± 0.037	2.344AlaArg: 2.344 ± 0.052	3.656AlaSer: 3.656 ± 0.052	3.541AlaThr: 3.541 ± 0.055	5.184AlaVal: 5.184 ± 0.072	0.453AlaTrp: 0.453 ± 0.02	2.378AlaTyr: 2.378 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.521CysAla: 0.521 ± 0.024	0.2CysCys: 0.2 ± 0.014	0.537CysAsp: 0.537 ± 0.018	0.61CysGlu: 0.61 ± 0.026	0.415CysPhe: 0.415 ± 0.018	0.923CysGly: 0.923 ± 0.034	0.222CysHis: 0.222 ± 0.015	0.913CysIle: 0.913 ± 0.031	0.722CysLys: 0.722 ± 0.027	0.729CysLeu: 0.729 ± 0.025	0.262CysMet: 0.262 ± 0.016	0.513CysAsn: 0.513 ± 0.026	0.467CysPro: 0.467 ± 0.021	0.304CysGln: 0.304 ± 0.016	0.413CysArg: 0.413 ± 0.019	0.678CysSer: 0.678 ± 0.022	0.465CysThr: 0.465 ± 0.021	0.562CysVal: 0.562 ± 0.024	0.066CysTrp: 0.066 ± 0.007	0.381CysTyr: 0.381 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.131AspAla: 3.131 ± 0.053	0.556AspCys: 0.556 ± 0.023	2.061AspAsp: 2.061 ± 0.049	3.914AspGlu: 3.914 ± 0.07	2.546AspPhe: 2.546 ± 0.042	3.001AspGly: 3.001 ± 0.066	1.024AspHis: 1.024 ± 0.029	5.325AspIle: 5.325 ± 0.067	3.716AspLys: 3.716 ± 0.059	5.102AspLeu: 5.102 ± 0.062	1.504AspMet: 1.504 ± 0.035	1.968AspAsn: 1.968 ± 0.053	1.675AspPro: 1.675 ± 0.039	1.47AspGln: 1.47 ± 0.036	2.01AspArg: 2.01 ± 0.046	2.4AspSer: 2.4 ± 0.044	2.618AspThr: 2.618 ± 0.049	3.471AspVal: 3.471 ± 0.054	0.462AspTrp: 0.462 ± 0.019	2.34AspTyr: 2.34 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	5.59GluAla: 5.59 ± 0.072	0.479GluCys: 0.479 ± 0.025	4.438GluAsp: 4.438 ± 0.068	8.66GluGlu: 8.66 ± 0.121	2.529GluPhe: 2.529 ± 0.053	5.265GluGly: 5.265 ± 0.067	1.104GluHis: 1.104 ± 0.03	7.573GluIle: 7.573 ± 0.077	7.775GluLys: 7.775 ± 0.099	6.507GluLeu: 6.507 ± 0.076	2.354GluMet: 2.354 ± 0.049	4.369GluAsn: 4.369 ± 0.058	1.514GluPro: 1.514 ± 0.041	1.827GluGln: 1.827 ± 0.04	2.815GluArg: 2.815 ± 0.056	3.192GluSer: 3.192 ± 0.048	3.507GluThr: 3.507 ± 0.058	6.108GluVal: 6.108 ± 0.086	0.467GluTrp: 0.467 ± 0.021	2.439GluTyr: 2.439 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	2.436PheAla: 2.436 ± 0.049	0.455PheCys: 0.455 ± 0.017	2.185PheAsp: 2.185 ± 0.046	2.532PheGlu: 2.532 ± 0.046	2.008PhePhe: 2.008 ± 0.048	2.724PheGly: 2.724 ± 0.05	0.867PheHis: 0.867 ± 0.027	4.217PheIle: 4.217 ± 0.075	2.726PheLys: 2.726 ± 0.052	4.054PheLeu: 4.054 ± 0.075	1.175PheMet: 1.175 ± 0.031	1.911PheAsn: 1.911 ± 0.038	1.374PhePro: 1.374 ± 0.029	1.537PheGln: 1.537 ± 0.04	1.396PheArg: 1.396 ± 0.036	2.868PheSer: 2.868 ± 0.06	2.438PheThr: 2.438 ± 0.047	2.736PheVal: 2.736 ± 0.052	0.321PheTrp: 0.321 ± 0.017	1.652PheTyr: 1.652 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.551GlyAla: 4.551 ± 0.074	0.888GlyCys: 0.888 ± 0.034	3.131GlyAsp: 3.131 ± 0.062	4.772GlyGlu: 4.772 ± 0.068	3.18GlyPhe: 3.18 ± 0.056	4.86GlyGly: 4.86 ± 0.08	1.183GlyHis: 1.183 ± 0.035	6.859GlyIle: 6.859 ± 0.099	5.231GlyLys: 5.231 ± 0.077	6.245GlyLeu: 6.245 ± 0.083	2.123GlyMet: 2.123 ± 0.044	2.976GlyAsn: 2.976 ± 0.055	1.54GlyPro: 1.54 ± 0.093	1.851GlyGln: 1.851 ± 0.043	2.637GlyArg: 2.637 ± 0.057	3.677GlySer: 3.677 ± 0.067	3.729GlyThr: 3.729 ± 0.062	4.84GlyVal: 4.84 ± 0.064	0.611GlyTrp: 0.611 ± 0.021	2.859GlyTyr: 2.859 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	0.989HisAla: 0.989 ± 0.031	0.247HisCys: 0.247 ± 0.015	0.789HisAsp: 0.789 ± 0.027	1.124HisGlu: 1.124 ± 0.033	0.777HisPhe: 0.777 ± 0.028	1.208HisGly: 1.208 ± 0.033	0.505HisHis: 0.505 ± 0.023	1.627HisIle: 1.627 ± 0.039	1.19HisLys: 1.19 ± 0.031	1.76HisLeu: 1.76 ± 0.04	0.472HisMet: 0.472 ± 0.021	0.802HisAsn: 0.802 ± 0.024	0.948HisPro: 0.948 ± 0.029	0.743HisGln: 0.743 ± 0.024	0.816HisArg: 0.816 ± 0.028	1.04HisSer: 1.04 ± 0.032	0.912HisThr: 0.912 ± 0.033	1.001HisVal: 1.001 ± 0.03	0.175HisTrp: 0.175 ± 0.012	0.745HisTyr: 0.745 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.604IleAla: 6.604 ± 0.08	0.907IleCys: 0.907 ± 0.03	5.341IleAsp: 5.341 ± 0.076	7.37IleGlu: 7.37 ± 0.091	3.791IlePhe: 3.791 ± 0.062	6.358IleGly: 6.358 ± 0.08	1.704IleHis: 1.704 ± 0.037	7.921IleIle: 7.921 ± 0.095	6.786IleLys: 6.786 ± 0.087	8.75IleLeu: 8.75 ± 0.11	2.289IleMet: 2.289 ± 0.041	4.366IleAsn: 4.366 ± 0.068	3.46IlePro: 3.46 ± 0.058	2.902IleGln: 2.902 ± 0.05	3.338IleArg: 3.338 ± 0.061	5.666IleSer: 5.666 ± 0.079	5.087IleThr: 5.087 ± 0.08	6.074IleVal: 6.074 ± 0.072	0.582IleTrp: 0.582 ± 0.026	3.131IleTyr: 3.131 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.806LysAla: 4.806 ± 0.065	0.585LysCys: 0.585 ± 0.026	4.439LysAsp: 4.439 ± 0.065	7.98LysGlu: 7.98 ± 0.109	2.216LysPhe: 2.216 ± 0.042	4.804LysGly: 4.804 ± 0.063	1.258LysHis: 1.258 ± 0.032	7.004LysIle: 7.004 ± 0.075	7.193LysLys: 7.193 ± 0.091	6.575LysLeu: 6.575 ± 0.084	2.064LysMet: 2.064 ± 0.042	4.475LysAsn: 4.475 ± 0.065	2.095LysPro: 2.095 ± 0.049	2.482LysGln: 2.482 ± 0.047	2.887LysArg: 2.887 ± 0.05	3.999LysSer: 3.999 ± 0.059	4.007LysThr: 4.007 ± 0.052	5.549LysVal: 5.549 ± 0.072	0.548LysTrp: 0.548 ± 0.021	2.921LysTyr: 2.921 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	6.273LeuAla: 6.273 ± 0.077	0.939LeuCys: 0.939 ± 0.032	4.74LeuAsp: 4.74 ± 0.073	7.28LeuGlu: 7.28 ± 0.091	3.813LeuPhe: 3.813 ± 0.071	6.692LeuGly: 6.692 ± 0.094	1.621LeuHis: 1.621 ± 0.035	7.657LeuIle: 7.657 ± 0.087	7.808LeuLys: 7.808 ± 0.106	9.807LeuLeu: 9.807 ± 0.112	2.714LeuMet: 2.714 ± 0.042	4.483LeuAsn: 4.483 ± 0.062	3.45LeuPro: 3.45 ± 0.064	3.7LeuGln: 3.7 ± 0.067	3.763LeuArg: 3.763 ± 0.062	6.311LeuSer: 6.311 ± 0.083	4.974LeuThr: 4.974 ± 0.06	6.01LeuVal: 6.01 ± 0.082	0.725LeuTrp: 0.725 ± 0.028	3.128LeuTyr: 3.128 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.07MetAla: 2.07 ± 0.045	0.207MetCys: 0.207 ± 0.012	1.454MetAsp: 1.454 ± 0.036	2.279MetGlu: 2.279 ± 0.047	0.939MetPhe: 0.939 ± 0.028	2.077MetGly: 2.077 ± 0.041	0.402MetHis: 0.402 ± 0.02	2.599MetIle: 2.599 ± 0.055	2.67MetLys: 2.67 ± 0.044	2.605MetLeu: 2.605 ± 0.043	1.07MetMet: 1.07 ± 0.03	1.443MetAsn: 1.443 ± 0.032	0.967MetPro: 0.967 ± 0.03	0.894MetGln: 0.894 ± 0.03	1.002MetArg: 1.002 ± 0.03	1.447MetSer: 1.447 ± 0.041	1.488MetThr: 1.488 ± 0.038	1.958MetVal: 1.958 ± 0.046	0.153MetTrp: 0.153 ± 0.012	0.763MetTyr: 0.763 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.681AsnAla: 2.681 ± 0.048	0.49AsnCys: 0.49 ± 0.025	1.974AsnAsp: 1.974 ± 0.041	3.012AsnGlu: 3.012 ± 0.062	2.04AsnPhe: 2.04 ± 0.043	2.608AsnGly: 2.608 ± 0.06	1.035AsnHis: 1.035 ± 0.031	5.096AsnIle: 5.096 ± 0.069	3.914AsnLys: 3.914 ± 0.058	4.804AsnLeu: 4.804 ± 0.068	1.334AsnMet: 1.334 ± 0.031	2.542AsnAsn: 2.542 ± 0.055	2.158AsnPro: 2.158 ± 0.052	1.822AsnGln: 1.822 ± 0.041	1.938AsnArg: 1.938 ± 0.041	2.514AsnSer: 2.514 ± 0.061	2.667AsnThr: 2.667 ± 0.051	2.808AsnVal: 2.808 ± 0.052	0.387AsnTrp: 0.387 ± 0.017	2.068AsnTyr: 2.068 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	1.936ProAla: 1.936 ± 0.046	0.316ProCys: 0.316 ± 0.017	1.553ProAsp: 1.553 ± 0.037	2.604ProGlu: 2.604 ± 0.049	1.461ProPhe: 1.461 ± 0.037	2.006ProGly: 2.006 ± 0.05	0.65ProHis: 0.65 ± 0.024	2.971ProIle: 2.971 ± 0.049	2.125ProLys: 2.125 ± 0.044	3.063ProLeu: 3.063 ± 0.055	0.895ProMet: 0.895 ± 0.027	1.448ProAsn: 1.448 ± 0.035	0.939ProPro: 0.939 ± 0.034	1.313ProGln: 1.313 ± 0.037	1.029ProArg: 1.029 ± 0.029	1.903ProSer: 1.903 ± 0.044	1.849ProThr: 1.849 ± 0.041	2.395ProVal: 2.395 ± 0.056	0.295ProTrp: 0.295 ± 0.016	1.353ProTyr: 1.353 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.006GlnAla: 2.006 ± 0.045	0.303GlnCys: 0.303 ± 0.017	1.535GlnAsp: 1.535 ± 0.036	3.084GlnGlu: 3.084 ± 0.057	1.119GlnPhe: 1.119 ± 0.031	2.131GlnGly: 2.131 ± 0.047	0.695GlnHis: 0.695 ± 0.024	2.329GlnIle: 2.329 ± 0.045	2.513GlnLys: 2.513 ± 0.055	3.282GlnLeu: 3.282 ± 0.056	0.94GlnMet: 0.94 ± 0.029	1.505GlnAsn: 1.505 ± 0.034	0.917GlnPro: 0.917 ± 0.026	1.81GlnGln: 1.81 ± 0.046	1.403GlnArg: 1.403 ± 0.032	1.693GlnSer: 1.693 ± 0.045	1.366GlnThr: 1.366 ± 0.033	2.229GlnVal: 2.229 ± 0.04	0.386GlnTrp: 0.386 ± 0.018	1.412GlnTyr: 1.412 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.19ArgAla: 2.19 ± 0.039	0.38ArgCys: 0.38 ± 0.017	2.043ArgAsp: 2.043 ± 0.044	3.324ArgGlu: 3.324 ± 0.049	1.558ArgPhe: 1.558 ± 0.036	2.434ArgGly: 2.434 ± 0.045	0.633ArgHis: 0.633 ± 0.024	3.395ArgIle: 3.395 ± 0.058	3.09ArgLys: 3.09 ± 0.053	3.554ArgLeu: 3.554 ± 0.06	1.097ArgMet: 1.097 ± 0.031	1.998ArgAsn: 1.998 ± 0.044	1.096ArgPro: 1.096 ± 0.03	1.412ArgGln: 1.412 ± 0.036	1.755ArgArg: 1.755 ± 0.038	1.866ArgSer: 1.866 ± 0.042	1.819ArgThr: 1.819 ± 0.046	2.604ArgVal: 2.604 ± 0.049	0.315ArgTrp: 0.315 ± 0.018	1.453ArgTyr: 1.453 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.068SerAla: 3.068 ± 0.055	0.557SerCys: 0.557 ± 0.022	2.505SerAsp: 2.505 ± 0.047	3.424SerGlu: 3.424 ± 0.056	2.883SerPhe: 2.883 ± 0.057	3.943SerGly: 3.943 ± 0.069	1.119SerHis: 1.119 ± 0.034	5.755SerIle: 5.755 ± 0.066	4.152SerLys: 4.152 ± 0.057	5.69SerLeu: 5.69 ± 0.067	1.641SerMet: 1.641 ± 0.043	2.778SerAsn: 2.778 ± 0.05	1.883SerPro: 1.883 ± 0.042	1.879SerGln: 1.879 ± 0.043	2.205SerArg: 2.205 ± 0.045	3.668SerSer: 3.668 ± 0.071	3.116SerThr: 3.116 ± 0.054	3.543SerVal: 3.543 ± 0.06	0.471SerTrp: 0.471 ± 0.022	2.195SerTyr: 2.195 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	3.753ThrAla: 3.753 ± 0.057	0.465ThrCys: 0.465 ± 0.02	2.453ThrAsp: 2.453 ± 0.039	3.453ThrGlu: 3.453 ± 0.059	2.274ThrPhe: 2.274 ± 0.041	4.115ThrGly: 4.115 ± 0.071	0.886ThrHis: 0.886 ± 0.027	4.95ThrIle: 4.95 ± 0.074	3.546ThrLys: 3.546 ± 0.054	5.287ThrLeu: 5.287 ± 0.068	1.39ThrMet: 1.39 ± 0.036	2.422ThrAsn: 2.422 ± 0.055	2.088ThrPro: 2.088 ± 0.046	1.401ThrGln: 1.401 ± 0.035	1.883ThrArg: 1.883 ± 0.042	3.107ThrSer: 3.107 ± 0.056	3.043ThrThr: 3.043 ± 0.053	3.895ThrVal: 3.895 ± 0.073	0.377ThrTrp: 0.377 ± 0.02	1.915ThrTyr: 1.915 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	4.921ValAla: 4.921 ± 0.077	0.7ValCys: 0.7 ± 0.028	3.75ValAsp: 3.75 ± 0.062	5.564ValGlu: 5.564 ± 0.067	3.138ValPhe: 3.138 ± 0.053	4.712ValGly: 4.712 ± 0.078	1.053ValHis: 1.053 ± 0.03	6.021ValIle: 6.021 ± 0.076	4.937ValLys: 4.937 ± 0.063	6.777ValLeu: 6.777 ± 0.076	1.958ValMet: 1.958 ± 0.039	2.993ValAsn: 2.993 ± 0.052	2.23ValPro: 2.23 ± 0.04	1.805ValGln: 1.805 ± 0.038	2.384ValArg: 2.384 ± 0.05	4.11ValSer: 4.11 ± 0.057	3.713ValThr: 3.713 ± 0.07	5.403ValVal: 5.403 ± 0.088	0.469ValTrp: 0.469 ± 0.02	2.363ValTyr: 2.363 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.424TrpAla: 0.424 ± 0.02	0.083TrpCys: 0.083 ± 0.01	0.434TrpAsp: 0.434 ± 0.02	0.577TrpGlu: 0.577 ± 0.025	0.311TrpPhe: 0.311 ± 0.016	0.587TrpGly: 0.587 ± 0.023	0.127TrpHis: 0.127 ± 0.011	0.64TrpIle: 0.64 ± 0.025	0.528TrpLys: 0.528 ± 0.024	0.72TrpLeu: 0.72 ± 0.027	0.267TrpMet: 0.267 ± 0.016	0.398TrpAsn: 0.398 ± 0.02	0.194TrpPro: 0.194 ± 0.016	0.317TrpGln: 0.317 ± 0.017	0.363TrpArg: 0.363 ± 0.017	0.45TrpSer: 0.45 ± 0.022	0.349TrpThr: 0.349 ± 0.02	0.488TrpVal: 0.488 ± 0.024	0.108TrpTrp: 0.108 ± 0.01	0.31TrpTyr: 0.31 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.034TyrAla: 2.034 ± 0.038	0.455TyrCys: 0.455 ± 0.023	2.107TyrAsp: 2.107 ± 0.044	2.608TyrGlu: 2.608 ± 0.052	1.749TyrPhe: 1.749 ± 0.045	2.622TyrGly: 2.622 ± 0.052	0.815TyrHis: 0.815 ± 0.027	3.356TyrIle: 3.356 ± 0.06	2.684TyrLys: 2.684 ± 0.053	3.634TyrLeu: 3.634 ± 0.059	0.883TyrMet: 0.883 ± 0.029	1.897TyrAsn: 1.897 ± 0.044	1.349TyrPro: 1.349 ± 0.036	1.285TyrGln: 1.285 ± 0.04	1.638TyrArg: 1.638 ± 0.032	2.229TyrSer: 2.229 ± 0.051	2.016TyrThr: 2.016 ± 0.041	2.144TyrVal: 2.144 ± 0.04	0.305TyrTrp: 0.305 ± 0.015	1.691TyrTyr: 1.691 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3835 proteins (1179242 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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