Sclerotinia sclerotiorum mitovirus 3
Average proteome isoelectric point is 8.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S4GCV3|A0A0S4GCV3_9VIRU RNA dependent RNA polymerase OS=Sclerotinia sclerotiorum mitovirus 3 OX=1279099 GN=RdRp PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.03 HH3 pKa = 6.45 LKK5 pKa = 8.74 LTTYY9 pKa = 10.56 KK10 pKa = 10.76 HH11 pKa = 6.66 LDD13 pKa = 3.53 LNINQADD20 pKa = 4.12 RR21 pKa = 11.84 FSNGAFAALSLKK33 pKa = 10.13 LWYY36 pKa = 9.22 PYY38 pKa = 10.55 VRR40 pKa = 11.84 LLIWSLRR47 pKa = 11.84 LDD49 pKa = 3.78 KK50 pKa = 10.92 PSFMKK55 pKa = 10.34 FATRR59 pKa = 11.84 IEE61 pKa = 4.33 SLWIHH66 pKa = 6.41 NGLIFTVKK74 pKa = 10.19 YY75 pKa = 10.55 LKK77 pKa = 10.31 EE78 pKa = 3.9 CLRR81 pKa = 11.84 ICQHH85 pKa = 5.93 FVSGNPVLVTTEE97 pKa = 3.94 MPISIVRR104 pKa = 11.84 GIPTIIPGSIRR115 pKa = 11.84 LQMHH119 pKa = 5.9 SRR121 pKa = 11.84 DD122 pKa = 3.39 LSVIRR127 pKa = 11.84 GVLSLLSVFRR137 pKa = 11.84 IMKK140 pKa = 9.95 IPSTLKK146 pKa = 10.56 LEE148 pKa = 4.94 SITGPFTGLDD158 pKa = 3.39 STLPKK163 pKa = 10.64 YY164 pKa = 10.43 EE165 pKa = 4.63 LIKK168 pKa = 10.38 IKK170 pKa = 10.37 QVLPVFPQIKK180 pKa = 9.66 PIRR183 pKa = 11.84 LKK185 pKa = 10.71 LLRR188 pKa = 11.84 SAGPNCKK195 pKa = 10.08 VSMLGIWLDD204 pKa = 3.35 IKK206 pKa = 10.92 AWSQHH211 pKa = 5.63 SNFKK215 pKa = 9.68 TLIEE219 pKa = 4.79 FIQLFPNYY227 pKa = 10.25 VEE229 pKa = 4.21 FRR231 pKa = 11.84 DD232 pKa = 3.74 MLLQEE237 pKa = 4.94 AYY239 pKa = 10.35 NLRR242 pKa = 11.84 LSVPSKK248 pKa = 10.52 DD249 pKa = 3.3 LHH251 pKa = 6.75 LGKK254 pKa = 10.37 LAIKK258 pKa = 10.28 EE259 pKa = 3.98 EE260 pKa = 4.33 AAGKK264 pKa = 10.41 ARR266 pKa = 11.84 VFAITDD272 pKa = 3.99 SITQSVMGPISDD284 pKa = 5.79 AIFKK288 pKa = 8.81 MLRR291 pKa = 11.84 QIPMDD296 pKa = 3.32 GTFNQSAPLDD306 pKa = 3.66 RR307 pKa = 11.84 LVQLSKK313 pKa = 11.16 DD314 pKa = 3.4 GAIAEE319 pKa = 4.42 KK320 pKa = 10.9 DD321 pKa = 3.71 RR322 pKa = 11.84 IFYY325 pKa = 10.75 SYY327 pKa = 10.86 DD328 pKa = 2.84 LSAATDD334 pKa = 3.56 RR335 pKa = 11.84 LPINLQKK342 pKa = 10.85 DD343 pKa = 3.3 ILSIYY348 pKa = 9.5 CGEE351 pKa = 4.22 SFASKK356 pKa = 9.95 WSILMTDD363 pKa = 3.87 RR364 pKa = 11.84 DD365 pKa = 3.63 WYY367 pKa = 8.87 LTKK370 pKa = 10.51 EE371 pKa = 4.09 KK372 pKa = 10.8 RR373 pKa = 11.84 NLRR376 pKa = 11.84 YY377 pKa = 9.81 SVGQPMGALSSWAMLALTHH396 pKa = 6.16 HH397 pKa = 7.22 FIVGMAANRR406 pKa = 11.84 VGKK409 pKa = 10.47 LGFNHH414 pKa = 6.26 YY415 pKa = 10.85 ALLGDD420 pKa = 5.56 DD421 pKa = 4.49 IVIADD426 pKa = 3.96 KK427 pKa = 11.17 SVADD431 pKa = 4.06 SYY433 pKa = 11.42 YY434 pKa = 10.37 MIMTEE439 pKa = 3.8 ILGVQINLSKK449 pKa = 11.1 SLVSTNSFEE458 pKa = 4.1 FAKK461 pKa = 10.7 RR462 pKa = 11.84 LVTLEE467 pKa = 4.31 GEE469 pKa = 4.46 VTPAGPANILLGLRR483 pKa = 11.84 SLNGIPSIILDD494 pKa = 3.88 MVNKK498 pKa = 9.33 GVPVSEE504 pKa = 4.72 EE505 pKa = 4.09 SLEE508 pKa = 4.18 SWMSTIPTVRR518 pKa = 11.84 KK519 pKa = 9.46 SQLEE523 pKa = 4.03 KK524 pKa = 10.91 VKK526 pKa = 10.18 WVVKK530 pKa = 10.5 GPFGFVPTAEE540 pKa = 4.32 GLASFLTMSSSLTPVRR556 pKa = 11.84 ANQIIHH562 pKa = 6.58 AVRR565 pKa = 11.84 YY566 pKa = 7.59 TKK568 pKa = 10.03 FQWDD572 pKa = 3.26 LDD574 pKa = 3.55 MWQKK578 pKa = 10.23 VVKK581 pKa = 10.7 DD582 pKa = 3.89 NINYY586 pKa = 9.68 RR587 pKa = 11.84 ISIEE591 pKa = 3.88 EE592 pKa = 4.35 LYY594 pKa = 10.89 FPVGFQDD601 pKa = 3.46 YY602 pKa = 9.58 PLDD605 pKa = 3.9 FRR607 pKa = 11.84 FSPIKK612 pKa = 10.1 MEE614 pKa = 4.71 IIKK617 pKa = 10.62 SLSQDD622 pKa = 3.17 LRR624 pKa = 11.84 DD625 pKa = 4.42 LGGQRR630 pKa = 11.84 PTFRR634 pKa = 11.84 LIFEE638 pKa = 4.73 GPIIMFNYY646 pKa = 8.56 YY647 pKa = 9.0 RR648 pKa = 11.84 QGYY651 pKa = 9.16 ASEE654 pKa = 3.7 IVNYY658 pKa = 9.88 IKK660 pKa = 10.92 YY661 pKa = 10.01 LVHH664 pKa = 6.42 QEE666 pKa = 4.07 PEE668 pKa = 4.21 FKK670 pKa = 10.31 IGGSDD675 pKa = 3.24 PFLPRR680 pKa = 11.84 NFEE683 pKa = 4.05 AFAFSSKK690 pKa = 10.5 FKK692 pKa = 9.81 GQKK695 pKa = 9.46 FFEE698 pKa = 4.37 QVRR701 pKa = 11.84 EE702 pKa = 4.0 HH703 pKa = 6.69 LRR705 pKa = 11.84 ANQLPMM711 pKa = 5.35
Molecular weight: 81.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.846
IPC2_protein 8.887
IPC_protein 8.785
Toseland 9.531
ProMoST 9.282
Dawson 9.794
Bjellqvist 9.487
Wikipedia 9.97
Rodwell 10.116
Grimsley 9.882
Solomon 9.809
Lehninger 9.78
Nozaki 9.575
DTASelect 9.472
Thurlkill 9.633
EMBOSS 9.955
Sillero 9.721
Patrickios 5.118
IPC_peptide 9.809
IPC2_peptide 7.995
IPC2.peptide.svr19 7.95
Protein with the highest isoelectric point:
>tr|A0A0S4GCV3|A0A0S4GCV3_9VIRU RNA dependent RNA polymerase OS=Sclerotinia sclerotiorum mitovirus 3 OX=1279099 GN=RdRp PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.03 HH3 pKa = 6.45 LKK5 pKa = 8.74 LTTYY9 pKa = 10.56 KK10 pKa = 10.76 HH11 pKa = 6.66 LDD13 pKa = 3.53 LNINQADD20 pKa = 4.12 RR21 pKa = 11.84 FSNGAFAALSLKK33 pKa = 10.13 LWYY36 pKa = 9.22 PYY38 pKa = 10.55 VRR40 pKa = 11.84 LLIWSLRR47 pKa = 11.84 LDD49 pKa = 3.78 KK50 pKa = 10.92 PSFMKK55 pKa = 10.34 FATRR59 pKa = 11.84 IEE61 pKa = 4.33 SLWIHH66 pKa = 6.41 NGLIFTVKK74 pKa = 10.19 YY75 pKa = 10.55 LKK77 pKa = 10.31 EE78 pKa = 3.9 CLRR81 pKa = 11.84 ICQHH85 pKa = 5.93 FVSGNPVLVTTEE97 pKa = 3.94 MPISIVRR104 pKa = 11.84 GIPTIIPGSIRR115 pKa = 11.84 LQMHH119 pKa = 5.9 SRR121 pKa = 11.84 DD122 pKa = 3.39 LSVIRR127 pKa = 11.84 GVLSLLSVFRR137 pKa = 11.84 IMKK140 pKa = 9.95 IPSTLKK146 pKa = 10.56 LEE148 pKa = 4.94 SITGPFTGLDD158 pKa = 3.39 STLPKK163 pKa = 10.64 YY164 pKa = 10.43 EE165 pKa = 4.63 LIKK168 pKa = 10.38 IKK170 pKa = 10.37 QVLPVFPQIKK180 pKa = 9.66 PIRR183 pKa = 11.84 LKK185 pKa = 10.71 LLRR188 pKa = 11.84 SAGPNCKK195 pKa = 10.08 VSMLGIWLDD204 pKa = 3.35 IKK206 pKa = 10.92 AWSQHH211 pKa = 5.63 SNFKK215 pKa = 9.68 TLIEE219 pKa = 4.79 FIQLFPNYY227 pKa = 10.25 VEE229 pKa = 4.21 FRR231 pKa = 11.84 DD232 pKa = 3.74 MLLQEE237 pKa = 4.94 AYY239 pKa = 10.35 NLRR242 pKa = 11.84 LSVPSKK248 pKa = 10.52 DD249 pKa = 3.3 LHH251 pKa = 6.75 LGKK254 pKa = 10.37 LAIKK258 pKa = 10.28 EE259 pKa = 3.98 EE260 pKa = 4.33 AAGKK264 pKa = 10.41 ARR266 pKa = 11.84 VFAITDD272 pKa = 3.99 SITQSVMGPISDD284 pKa = 5.79 AIFKK288 pKa = 8.81 MLRR291 pKa = 11.84 QIPMDD296 pKa = 3.32 GTFNQSAPLDD306 pKa = 3.66 RR307 pKa = 11.84 LVQLSKK313 pKa = 11.16 DD314 pKa = 3.4 GAIAEE319 pKa = 4.42 KK320 pKa = 10.9 DD321 pKa = 3.71 RR322 pKa = 11.84 IFYY325 pKa = 10.75 SYY327 pKa = 10.86 DD328 pKa = 2.84 LSAATDD334 pKa = 3.56 RR335 pKa = 11.84 LPINLQKK342 pKa = 10.85 DD343 pKa = 3.3 ILSIYY348 pKa = 9.5 CGEE351 pKa = 4.22 SFASKK356 pKa = 9.95 WSILMTDD363 pKa = 3.87 RR364 pKa = 11.84 DD365 pKa = 3.63 WYY367 pKa = 8.87 LTKK370 pKa = 10.51 EE371 pKa = 4.09 KK372 pKa = 10.8 RR373 pKa = 11.84 NLRR376 pKa = 11.84 YY377 pKa = 9.81 SVGQPMGALSSWAMLALTHH396 pKa = 6.16 HH397 pKa = 7.22 FIVGMAANRR406 pKa = 11.84 VGKK409 pKa = 10.47 LGFNHH414 pKa = 6.26 YY415 pKa = 10.85 ALLGDD420 pKa = 5.56 DD421 pKa = 4.49 IVIADD426 pKa = 3.96 KK427 pKa = 11.17 SVADD431 pKa = 4.06 SYY433 pKa = 11.42 YY434 pKa = 10.37 MIMTEE439 pKa = 3.8 ILGVQINLSKK449 pKa = 11.1 SLVSTNSFEE458 pKa = 4.1 FAKK461 pKa = 10.7 RR462 pKa = 11.84 LVTLEE467 pKa = 4.31 GEE469 pKa = 4.46 VTPAGPANILLGLRR483 pKa = 11.84 SLNGIPSIILDD494 pKa = 3.88 MVNKK498 pKa = 9.33 GVPVSEE504 pKa = 4.72 EE505 pKa = 4.09 SLEE508 pKa = 4.18 SWMSTIPTVRR518 pKa = 11.84 KK519 pKa = 9.46 SQLEE523 pKa = 4.03 KK524 pKa = 10.91 VKK526 pKa = 10.18 WVVKK530 pKa = 10.5 GPFGFVPTAEE540 pKa = 4.32 GLASFLTMSSSLTPVRR556 pKa = 11.84 ANQIIHH562 pKa = 6.58 AVRR565 pKa = 11.84 YY566 pKa = 7.59 TKK568 pKa = 10.03 FQWDD572 pKa = 3.26 LDD574 pKa = 3.55 MWQKK578 pKa = 10.23 VVKK581 pKa = 10.7 DD582 pKa = 3.89 NINYY586 pKa = 9.68 RR587 pKa = 11.84 ISIEE591 pKa = 3.88 EE592 pKa = 4.35 LYY594 pKa = 10.89 FPVGFQDD601 pKa = 3.46 YY602 pKa = 9.58 PLDD605 pKa = 3.9 FRR607 pKa = 11.84 FSPIKK612 pKa = 10.1 MEE614 pKa = 4.71 IIKK617 pKa = 10.62 SLSQDD622 pKa = 3.17 LRR624 pKa = 11.84 DD625 pKa = 4.42 LGGQRR630 pKa = 11.84 PTFRR634 pKa = 11.84 LIFEE638 pKa = 4.73 GPIIMFNYY646 pKa = 8.56 YY647 pKa = 9.0 RR648 pKa = 11.84 QGYY651 pKa = 9.16 ASEE654 pKa = 3.7 IVNYY658 pKa = 9.88 IKK660 pKa = 10.92 YY661 pKa = 10.01 LVHH664 pKa = 6.42 QEE666 pKa = 4.07 PEE668 pKa = 4.21 FKK670 pKa = 10.31 IGGSDD675 pKa = 3.24 PFLPRR680 pKa = 11.84 NFEE683 pKa = 4.05 AFAFSSKK690 pKa = 10.5 FKK692 pKa = 9.81 GQKK695 pKa = 9.46 FFEE698 pKa = 4.37 QVRR701 pKa = 11.84 EE702 pKa = 4.0 HH703 pKa = 6.69 LRR705 pKa = 11.84 ANQLPMM711 pKa = 5.35
Molecular weight: 81.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.846
IPC2_protein 8.887
IPC_protein 8.785
Toseland 9.531
ProMoST 9.282
Dawson 9.794
Bjellqvist 9.487
Wikipedia 9.97
Rodwell 10.116
Grimsley 9.882
Solomon 9.809
Lehninger 9.78
Nozaki 9.575
DTASelect 9.472
Thurlkill 9.633
EMBOSS 9.955
Sillero 9.721
Patrickios 5.118
IPC_peptide 9.809
IPC2_peptide 7.995
IPC2.peptide.svr19 7.95
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
711
711
711
711.0
81.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.485 ± 0.0
0.563 ± 0.0
4.501 ± 0.0
4.501 ± 0.0
5.767 ± 0.0
5.485 ± 0.0
1.828 ± 0.0
8.579 ± 0.0
6.751 ± 0.0
11.814 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.235 ± 0.0
3.657 ± 0.0
5.204 ± 0.0
3.797 ± 0.0
5.204 ± 0.0
8.439 ± 0.0
4.219 ± 0.0
5.907 ± 0.0
1.688 ± 0.0
3.376 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here