Halorubrum sp. E3

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum; unclassified Halorubrum

Average proteome isoelectric point is 4.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4984 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A256I9R1|A0A256I9R1_9EURY Phage repressor protein OS=Halorubrum sp. E3 OX=1483400 GN=DJ71_27740 PE=4 SV=1
MM1 pKa = 7.12SQQGVQEE8 pKa = 4.12EE9 pKa = 5.68LYY11 pKa = 10.68VDD13 pKa = 4.12QYY15 pKa = 11.52TLGLVGPDD23 pKa = 3.48QEE25 pKa = 4.57WAGTVADD32 pKa = 4.47GGTVTTYY39 pKa = 9.98TPPGCWGPMVTPSFRR54 pKa = 11.84GGHH57 pKa = 5.06EE58 pKa = 3.75VTRR61 pKa = 11.84PIRR64 pKa = 11.84VEE66 pKa = 3.78GAEE69 pKa = 4.01VGDD72 pKa = 3.82AVAIHH77 pKa = 6.26IRR79 pKa = 11.84DD80 pKa = 3.73VEE82 pKa = 4.51VTSMATSTGSMAEE95 pKa = 3.97RR96 pKa = 11.84EE97 pKa = 4.53GAFGDD102 pKa = 4.66DD103 pKa = 3.43PFVDD107 pKa = 4.29HH108 pKa = 7.33RR109 pKa = 11.84CPEE112 pKa = 4.74CGTTWPDD119 pKa = 3.24SVVEE123 pKa = 4.23GTGEE127 pKa = 3.82DD128 pKa = 3.76AIRR131 pKa = 11.84CAEE134 pKa = 4.18CGANASSFGFEE145 pKa = 3.79YY146 pKa = 10.41GYY148 pKa = 8.93TVAFDD153 pKa = 3.68HH154 pKa = 6.64EE155 pKa = 4.52NAVGITLDD163 pKa = 3.33EE164 pKa = 5.25DD165 pKa = 4.29GAHH168 pKa = 6.6EE169 pKa = 4.35LALDD173 pKa = 4.01ADD175 pKa = 4.34EE176 pKa = 5.95VMDD179 pKa = 3.57IPEE182 pKa = 4.49NARR185 pKa = 11.84QHH187 pKa = 6.73PILLYY192 pKa = 10.55EE193 pKa = 4.45PDD195 pKa = 4.17GMPGTLGRR203 pKa = 11.84LRR205 pKa = 11.84PFIGNIGTTPPVTMPDD221 pKa = 2.99SHH223 pKa = 6.53NAGDD227 pKa = 4.37FGQNLIGADD236 pKa = 3.19HH237 pKa = 7.78DD238 pKa = 4.6YY239 pKa = 11.38GVEE242 pKa = 4.07TEE244 pKa = 4.48EE245 pKa = 5.77DD246 pKa = 3.45LEE248 pKa = 4.19QRR250 pKa = 11.84TDD252 pKa = 2.72GHH254 pKa = 6.05MDD256 pKa = 3.26VPEE259 pKa = 4.21VRR261 pKa = 11.84AGATLICPVVVDD273 pKa = 3.75GGGIYY278 pKa = 10.68VGDD281 pKa = 3.75LHH283 pKa = 7.3ANQGDD288 pKa = 4.47GEE290 pKa = 4.6LSLHH294 pKa = 5.12TTDD297 pKa = 3.8VSGTVTMDD305 pKa = 3.09VEE307 pKa = 4.55VIEE310 pKa = 5.8DD311 pKa = 3.54VDD313 pKa = 4.45LDD315 pKa = 4.47GPVLLPNEE323 pKa = 4.07EE324 pKa = 4.42DD325 pKa = 3.98LPFISAPYY333 pKa = 9.75SDD335 pKa = 4.78EE336 pKa = 3.89EE337 pKa = 4.43VAAGDD342 pKa = 4.45DD343 pKa = 3.6LGAEE347 pKa = 4.38HH348 pKa = 6.89GVDD351 pKa = 3.44VV352 pKa = 4.67

Molecular weight: 37.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A256I5Q1|A0A256I5Q1_9EURY S-adenosylmethionine synthetase (Fragment) OS=Halorubrum sp. E3 OX=1483400 GN=DJ71_28470 PE=4 SV=1
MM1 pKa = 7.62AATSGPLASLARR13 pKa = 11.84SRR15 pKa = 11.84SSVIRR20 pKa = 11.84SSLSPRR26 pKa = 11.84FALAASGRR34 pKa = 11.84SRR36 pKa = 11.84ATTALRR42 pKa = 11.84FASRR46 pKa = 11.84LIVSARR52 pKa = 11.84ALVSAMRR59 pKa = 11.84AANAPP64 pKa = 3.54

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4984

0

4984

1007089

24

1668

202.1

21.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.051 ± 0.061

0.692 ± 0.014

8.838 ± 0.047

8.581 ± 0.056

3.274 ± 0.028

8.595 ± 0.044

1.948 ± 0.017

4.108 ± 0.029

1.949 ± 0.027

8.64 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.696 ± 0.018

2.388 ± 0.026

4.67 ± 0.023

2.403 ± 0.021

6.64 ± 0.047

5.524 ± 0.03

6.24 ± 0.033

8.893 ± 0.041

1.129 ± 0.016

2.742 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski