Pseudomonas phage PMBT3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Maxrubnervirus; Pseudomonas virus PMBT3

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 116 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PHZ3|A0A2I6PHZ3_9CAUD Uncharacterized protein OS=Pseudomonas phage PMBT3 OX=2059856 PE=4 SV=1
MM1 pKa = 7.75AIIDD5 pKa = 3.76QIEE8 pKa = 4.11YY9 pKa = 10.24SVCEE13 pKa = 4.09DD14 pKa = 4.05CLLLIANGEE23 pKa = 4.01EE24 pKa = 4.57SEE26 pKa = 4.32NCDD29 pKa = 3.2GFHH32 pKa = 7.65VEE34 pKa = 3.93ACMKK38 pKa = 10.55RR39 pKa = 11.84EE40 pKa = 3.77LNGRR44 pKa = 11.84KK45 pKa = 8.7GHH47 pKa = 5.58WVAGVEE53 pKa = 4.16PTDD56 pKa = 3.71EE57 pKa = 4.8DD58 pKa = 4.16EE59 pKa = 4.77EE60 pKa = 4.82GTGYY64 pKa = 11.16EE65 pKa = 4.31EE66 pKa = 5.95FSGCNCEE73 pKa = 4.56LCNTHH78 pKa = 6.89LAGSRR83 pKa = 11.84HH84 pKa = 5.06GVSLLLEE91 pKa = 4.72GDD93 pKa = 4.48DD94 pKa = 5.5DD95 pKa = 4.52GQQ97 pKa = 4.46

Molecular weight: 10.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6PHT0|A0A2I6PHT0_9CAUD CRISPR-associated protein OS=Pseudomonas phage PMBT3 OX=2059856 PE=4 SV=1
MM1 pKa = 7.61GKK3 pKa = 9.1FAKK6 pKa = 9.9MADD9 pKa = 3.59GVNLDD14 pKa = 3.99LAALFSRR21 pKa = 11.84SIRR24 pKa = 11.84RR25 pKa = 11.84ALLAGLVIAVQTTVHH40 pKa = 6.8DD41 pKa = 4.25SSNAAAHH48 pKa = 6.46WLLAARR54 pKa = 11.84GKK56 pKa = 10.09SRR58 pKa = 11.84PGARR62 pKa = 11.84TWGKK66 pKa = 10.15LRR68 pKa = 11.84DD69 pKa = 3.67LRR71 pKa = 11.84GTSKK75 pKa = 10.64KK76 pKa = 10.54AATIPVGKK84 pKa = 10.27RR85 pKa = 11.84RR86 pKa = 11.84DD87 pKa = 3.58AGKK90 pKa = 9.87NAALTQRR97 pKa = 11.84FVRR100 pKa = 11.84DD101 pKa = 3.22RR102 pKa = 11.84EE103 pKa = 3.99LRR105 pKa = 11.84EE106 pKa = 3.95VVEE109 pKa = 4.24KK110 pKa = 10.74LVAGRR115 pKa = 11.84SPEE118 pKa = 4.18TVFYY122 pKa = 9.57FYY124 pKa = 11.26NAVEE128 pKa = 4.29PDD130 pKa = 3.31SEE132 pKa = 4.38YY133 pKa = 11.4GRR135 pKa = 11.84NANIQQAAAAARR147 pKa = 11.84EE148 pKa = 4.15EE149 pKa = 4.31VSRR152 pKa = 11.84QFEE155 pKa = 4.02NAMAAGQVRR164 pKa = 11.84KK165 pKa = 10.02KK166 pKa = 10.8YY167 pKa = 10.22RR168 pKa = 3.29

Molecular weight: 18.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

116

0

116

27183

39

1727

234.3

25.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.37 ± 0.526

1.019 ± 0.091

6.232 ± 0.173

6.622 ± 0.252

3.311 ± 0.141

7.339 ± 0.206

2.049 ± 0.166

5.154 ± 0.225

5.912 ± 0.25

8.641 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.131

3.903 ± 0.118

4.253 ± 0.277

3.962 ± 0.223

5.095 ± 0.173

5.286 ± 0.197

6.438 ± 0.252

7.001 ± 0.225

1.453 ± 0.132

3.075 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski