Profundibacter amoris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Profundibacter

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A347ULL6|A0A347ULL6_9RHOB DUF4198 domain-containing protein OS=Profundibacter amoris OX=2171755 GN=BAR1_08025 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 4.21NEE5 pKa = 4.66LNDD8 pKa = 3.95LDD10 pKa = 4.4GQISTFIADD19 pKa = 3.69EE20 pKa = 4.75LIAYY24 pKa = 9.04ADD26 pKa = 3.73QKK28 pKa = 11.49NVIIDD33 pKa = 3.78SIKK36 pKa = 10.38IGNEE40 pKa = 3.16YY41 pKa = 10.65DD42 pKa = 4.1LNGLSAAEE50 pKa = 4.05YY51 pKa = 9.57GQIASRR57 pKa = 11.84LSLIIGEE64 pKa = 4.69SLDD67 pKa = 3.67QYY69 pKa = 11.13GSSHH73 pKa = 6.57IGWGEE78 pKa = 3.58PKK80 pKa = 10.44LVVEE84 pKa = 5.38SGRR87 pKa = 11.84IWLQEE92 pKa = 4.01SGSGLFFGDD101 pKa = 3.44RR102 pKa = 11.84DD103 pKa = 3.99HH104 pKa = 7.11NGLMDD109 pKa = 3.89AQEE112 pKa = 4.31IKK114 pKa = 10.76DD115 pKa = 3.62AFDD118 pKa = 4.0TIEE121 pKa = 4.2AGYY124 pKa = 10.65VDD126 pKa = 4.75AVDD129 pKa = 3.38IHH131 pKa = 7.1SLTLFLSSYY140 pKa = 10.57EE141 pKa = 4.39DD142 pKa = 3.78YY143 pKa = 10.82FGQGPYY149 pKa = 11.03SNANLNTIYY158 pKa = 10.88SSLNSFWDD166 pKa = 3.44DD167 pKa = 3.11TFQDD171 pKa = 4.05VEE173 pKa = 4.38LHH175 pKa = 5.18TLAWQYY181 pKa = 10.18PWQRR185 pKa = 11.84DD186 pKa = 2.73IDD188 pKa = 4.2GPQGPGGVEE197 pKa = 3.85DD198 pKa = 4.28HH199 pKa = 6.83GGSALVNASLGFMQYY214 pKa = 11.1YY215 pKa = 9.85EE216 pKa = 4.16MSLGGVDD223 pKa = 4.73YY224 pKa = 10.84AVSWLASGWGGASPTLFHH242 pKa = 6.8NEE244 pKa = 3.28PRR246 pKa = 11.84AGGEE250 pKa = 4.06LFRR253 pKa = 11.84LMHH256 pKa = 6.25EE257 pKa = 4.7NISGLRR263 pKa = 11.84AIEE266 pKa = 4.16FDD268 pKa = 3.17QDD270 pKa = 3.27PTVFQNGDD278 pKa = 3.8DD279 pKa = 3.84IEE281 pKa = 5.07DD282 pKa = 3.49VVFRR286 pKa = 11.84AFEE289 pKa = 3.96QEE291 pKa = 4.23GKK293 pKa = 10.03VVLYY297 pKa = 10.27IGNLEE302 pKa = 4.35TEE304 pKa = 4.47SQTITIDD311 pKa = 3.95DD312 pKa = 3.74VLQFLEE318 pKa = 4.74GVDD321 pKa = 3.79GFGGFLNLNTTEE333 pKa = 4.99NIHH336 pKa = 5.62IWGSRR341 pKa = 11.84LGVDD345 pKa = 4.36GDD347 pKa = 4.15PNNYY351 pKa = 10.18SSMPVIDD358 pKa = 4.63IYY360 pKa = 11.69NHH362 pKa = 6.29IDD364 pKa = 3.41LFGDD368 pKa = 3.63SKK370 pKa = 11.68GFGALEE376 pKa = 4.17FEE378 pKa = 4.54LGAYY382 pKa = 9.89EE383 pKa = 4.79IMQLTFTSRR392 pKa = 11.84NHH394 pKa = 6.87DD395 pKa = 3.8LVEE398 pKa = 4.16MSGHH402 pKa = 6.28NGNDD406 pKa = 3.47TLIGSTWNDD415 pKa = 3.11EE416 pKa = 4.1LFGMGGNDD424 pKa = 3.77TIYY427 pKa = 11.08GGTGNDD433 pKa = 4.1AIYY436 pKa = 10.72GDD438 pKa = 4.25SGNDD442 pKa = 3.45EE443 pKa = 4.32LRR445 pKa = 11.84GDD447 pKa = 4.16AGNDD451 pKa = 3.58NISGGLGADD460 pKa = 3.69LVIGGTGNDD469 pKa = 3.88VISGGAYY476 pKa = 8.85GDD478 pKa = 3.73EE479 pKa = 4.31MFGGDD484 pKa = 4.54GFDD487 pKa = 5.09FINGGFGNDD496 pKa = 3.22RR497 pKa = 11.84LNGGEE502 pKa = 4.09GGDD505 pKa = 3.61KK506 pKa = 10.44FFHH509 pKa = 6.87LGIADD514 pKa = 4.56HH515 pKa = 6.66GSDD518 pKa = 4.31WIQDD522 pKa = 3.78YY523 pKa = 11.41NSVEE527 pKa = 4.07GDD529 pKa = 3.5VLLWGGGNATASDD542 pKa = 4.14FLVQRR547 pKa = 11.84AFTDD551 pKa = 3.46NAGDD555 pKa = 3.61ASVEE559 pKa = 4.37EE560 pKa = 4.44IFITHH565 pKa = 6.98IPTGNLLWALVDD577 pKa = 3.64GGEE580 pKa = 3.97QAEE583 pKa = 4.24INIQIGGEE591 pKa = 4.34VYY593 pKa = 10.94NLLL596 pKa = 4.23

Molecular weight: 64.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A347UCR7|A0A347UCR7_9RHOB Thiamine/thiamine pyrophosphate ABC transporter permease ThiP OS=Profundibacter amoris OX=2171755 GN=BAR1_01035 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.83HH14 pKa = 4.64RR15 pKa = 11.84HH16 pKa = 3.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3454

0

3454

1063791

35

3278

308.0

33.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.254 ± 0.045

0.889 ± 0.014

6.114 ± 0.034

5.835 ± 0.036

3.868 ± 0.031

8.468 ± 0.044

2.005 ± 0.022

5.697 ± 0.03

4.169 ± 0.036

9.815 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.987 ± 0.024

3.06 ± 0.025

4.869 ± 0.03

3.279 ± 0.023

6.056 ± 0.037

5.069 ± 0.029

5.515 ± 0.031

7.22 ± 0.038

1.373 ± 0.019

2.46 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski