Apis mellifera associated microvirus 41

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 8.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5W2|A0A3Q8U5W2_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 41 OX=2494771 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.06RR3 pKa = 11.84SKK5 pKa = 10.7FSLSHH10 pKa = 6.26YY11 pKa = 10.42KK12 pKa = 10.54LLSCDD17 pKa = 3.28MGEE20 pKa = 5.45LIPCGLQEE28 pKa = 4.61VLPGDD33 pKa = 4.24TFQQSTSILVRR44 pKa = 11.84ASPLLAPVMHH54 pKa = 6.83PVHH57 pKa = 6.76VRR59 pKa = 11.84IHH61 pKa = 5.67HH62 pKa = 5.87WFVPHH67 pKa = 6.64RR68 pKa = 11.84LVWEE72 pKa = 4.18DD73 pKa = 2.96WEE75 pKa = 4.29EE76 pKa = 4.51FITGGPDD83 pKa = 3.24GMDD86 pKa = 3.17DD87 pKa = 4.07SVFPTITAGGSGYY100 pKa = 9.79PVGSLSDD107 pKa = 3.83YY108 pKa = 10.94LGVPPGVANLEE119 pKa = 4.43HH120 pKa = 6.74SALPHH125 pKa = 6.38RR126 pKa = 11.84GYY128 pKa = 11.51GLIWNEE134 pKa = 3.74WYY136 pKa = 10.21RR137 pKa = 11.84DD138 pKa = 3.16QDD140 pKa = 3.6LQSEE144 pKa = 4.79VFVDD148 pKa = 3.75KK149 pKa = 11.06TSGPDD154 pKa = 3.2ANTSVALQNVGWEE167 pKa = 3.99KK168 pKa = 11.19DD169 pKa = 3.66YY170 pKa = 9.37FTSARR175 pKa = 11.84PWEE178 pKa = 4.41QKK180 pKa = 10.76GPTISIPVGGAAPVTGSVEE199 pKa = 4.04FLTNSTVRR207 pKa = 11.84TVSATGGTSGARR219 pKa = 11.84GMEE222 pKa = 3.77IPATNNPDD230 pKa = 3.11TVMARR235 pKa = 11.84LGADD239 pKa = 3.28AEE241 pKa = 4.56ADD243 pKa = 3.52LSGASAVNVNILRR256 pKa = 11.84EE257 pKa = 4.0ALALQRR263 pKa = 11.84YY264 pKa = 8.18EE265 pKa = 4.12EE266 pKa = 3.92ARR268 pKa = 11.84ARR270 pKa = 11.84YY271 pKa = 7.87GSRR274 pKa = 11.84YY275 pKa = 9.33VEE277 pKa = 3.92YY278 pKa = 10.67LRR280 pKa = 11.84YY281 pKa = 10.05LGVRR285 pKa = 11.84SSDD288 pKa = 3.15ARR290 pKa = 11.84LQRR293 pKa = 11.84PEE295 pKa = 3.94YY296 pKa = 10.49LGGGQATIQFSEE308 pKa = 4.39VLQTAEE314 pKa = 3.79GTNPVGEE321 pKa = 4.25LRR323 pKa = 11.84GHH325 pKa = 7.28GIGTPRR331 pKa = 11.84SNRR334 pKa = 11.84YY335 pKa = 8.58RR336 pKa = 11.84KK337 pKa = 9.26FFEE340 pKa = 3.63EE341 pKa = 3.66HH342 pKa = 6.59GYY344 pKa = 8.97IHH346 pKa = 6.6TFVSVRR352 pKa = 11.84PKK354 pKa = 9.96TIYY357 pKa = 10.75ANGLFRR363 pKa = 11.84HH364 pKa = 5.65WNRR367 pKa = 11.84RR368 pKa = 11.84TKK370 pKa = 10.55EE371 pKa = 4.11DD372 pKa = 2.95FWQRR376 pKa = 11.84EE377 pKa = 4.21LQHH380 pKa = 6.98IGQQEE385 pKa = 4.19VLNKK389 pKa = 9.71EE390 pKa = 4.23VYY392 pKa = 9.69AASASPDD399 pKa = 3.61DD400 pKa = 3.73VFGFQDD406 pKa = 3.41RR407 pKa = 11.84YY408 pKa = 10.77DD409 pKa = 3.49EE410 pKa = 4.06YY411 pKa = 11.43RR412 pKa = 11.84RR413 pKa = 11.84TEE415 pKa = 3.84SSIGGEE421 pKa = 4.02FRR423 pKa = 11.84TTLDD427 pKa = 3.33FWHH430 pKa = 7.02MARR433 pKa = 11.84IFSSSPALNADD444 pKa = 3.81FVKK447 pKa = 10.63SVPTEE452 pKa = 3.61RR453 pKa = 11.84TFAVPSEE460 pKa = 4.06DD461 pKa = 4.12VLWLMARR468 pKa = 11.84HH469 pKa = 6.41NIQARR474 pKa = 11.84RR475 pKa = 11.84MVAAQGHH482 pKa = 5.72SYY484 pKa = 10.71IFF486 pKa = 3.83

Molecular weight: 54.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U5L1|A0A3Q8U5L1_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 41 OX=2494771 PE=3 SV=1
MM1 pKa = 7.93AARR4 pKa = 11.84QAQPRR9 pKa = 11.84GCKK12 pKa = 9.27LCKK15 pKa = 10.12KK16 pKa = 8.53ITPSTTWMASRR27 pKa = 11.84FMMSGDD33 pKa = 3.44MKK35 pKa = 11.11SPIPRR40 pKa = 11.84RR41 pKa = 11.84SRR43 pKa = 11.84QRR45 pKa = 11.84SATSVSRR52 pKa = 11.84LSLTKK57 pKa = 10.13SGQWSGLNVSPRR69 pKa = 11.84KK70 pKa = 7.98RR71 pKa = 11.84QRR73 pKa = 11.84WALRR77 pKa = 11.84PSKK80 pKa = 10.43RR81 pKa = 11.84PTISRR86 pKa = 11.84SAIRR90 pKa = 11.84MTSFQALHH98 pKa = 6.57TSTISTPCPCPSSRR112 pKa = 11.84LVRR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84PKK119 pKa = 10.5RR120 pKa = 11.84PTRR123 pKa = 11.84LPLQSPNLRR132 pKa = 11.84KK133 pKa = 9.84NLATTTSRR141 pKa = 11.84RR142 pKa = 11.84LHH144 pKa = 5.87LRR146 pKa = 11.84RR147 pKa = 11.84EE148 pKa = 3.92RR149 pKa = 11.84DD150 pKa = 3.43LANSPAPVGAAPSAA164 pKa = 3.57

Molecular weight: 18.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1352

133

486

270.4

30.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.58 ± 0.805

1.331 ± 0.673

4.29 ± 0.789

4.438 ± 1.01

3.402 ± 0.538

7.618 ± 1.174

2.589 ± 0.35

3.254 ± 0.267

3.624 ± 0.782

8.136 ± 0.769

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.332

3.033 ± 0.247

6.065 ± 0.805

4.438 ± 0.912

10.429 ± 1.918

9.467 ± 1.476

5.399 ± 0.786

6.731 ± 0.942

1.701 ± 0.23

2.959 ± 0.597
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski