Staphylococcus phage SP197

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coventryvirus; Staphylococcus virus SP197

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494WED5|A0A494WED5_9CAUD Phage major tail protein OS=Staphylococcus phage SP197 OX=2495554 GN=SP197g_29 PE=4 SV=1
MM1 pKa = 7.69IKK3 pKa = 9.31RR4 pKa = 11.84TINLLITLALYY15 pKa = 10.26EE16 pKa = 4.56LGTYY20 pKa = 8.07LTEE23 pKa = 3.71QAIIILTANDD33 pKa = 3.79DD34 pKa = 3.21VDD36 pKa = 4.36AFNEE40 pKa = 3.91YY41 pKa = 11.24DD42 pKa = 4.95HH43 pKa = 7.44IDD45 pKa = 3.72LNDD48 pKa = 2.84IRR50 pKa = 11.84AEE52 pKa = 4.05VSGG55 pKa = 3.91

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494WCP9|A0A494WCP9_9CAUD Phage protein OS=Staphylococcus phage SP197 OX=2495554 GN=SP197g_18 PE=4 SV=1
MM1 pKa = 7.81NDD3 pKa = 3.48VTWKK7 pKa = 9.99DD8 pKa = 3.33LKK10 pKa = 11.13FIGFTRR16 pKa = 11.84SQKK19 pKa = 11.29AKK21 pKa = 9.09MIHH24 pKa = 6.36KK25 pKa = 10.39GITPSIALSRR35 pKa = 11.84YY36 pKa = 7.76KK37 pKa = 10.57NYY39 pKa = 10.04WSIEE43 pKa = 4.17EE44 pKa = 4.14IVNTKK49 pKa = 10.31PYY51 pKa = 8.75MKK53 pKa = 10.02RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84KK57 pKa = 9.6

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13034

47

1039

186.2

21.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.084 ± 0.378

0.468 ± 0.072

6.636 ± 0.375

7.549 ± 0.508

4.189 ± 0.209

5.079 ± 0.294

1.573 ± 0.16

7.465 ± 0.314

9.498 ± 0.405

8.179 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.126

6.491 ± 0.253

2.486 ± 0.188

3.583 ± 0.176

4.059 ± 0.192

6.061 ± 0.331

6.122 ± 0.251

6.652 ± 0.242

1.089 ± 0.137

4.373 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski