Streptococcus satellite phage Javan353
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKL3|A0A4D5ZKL3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan353 OX=2558654 GN=JavanS353_0002 PE=4 SV=1
MM1 pKa = 7.7 EE2 pKa = 5.23 IVEE5 pKa = 4.83 DD6 pKa = 4.34 CNSVITDD13 pKa = 3.9 LWLSFYY19 pKa = 10.86 KK20 pKa = 10.71 LSEE23 pKa = 4.12 AYY25 pKa = 9.6 KK26 pKa = 8.6 MQEE29 pKa = 3.54 ASHH32 pKa = 6.42 EE33 pKa = 4.0 EE34 pKa = 4.33 FYY36 pKa = 11.56 NKK38 pKa = 9.88 NVEE41 pKa = 4.11 NLLGEE46 pKa = 4.27 LKK48 pKa = 10.63 KK49 pKa = 10.94 YY50 pKa = 10.67 DD51 pKa = 3.93 DD52 pKa = 4.86 EE53 pKa = 4.52 CAEE56 pKa = 4.38 MYY58 pKa = 10.28 NKK60 pKa = 8.79 KK61 pKa = 7.88 TT62 pKa = 3.47
Molecular weight: 7.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.365
IPC2_protein 4.558
IPC_protein 4.393
Toseland 4.253
ProMoST 4.482
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.177
Rodwell 4.24
Grimsley 4.164
Solomon 4.317
Lehninger 4.266
Nozaki 4.444
DTASelect 4.533
Thurlkill 4.253
EMBOSS 4.19
Sillero 4.495
Patrickios 3.058
IPC_peptide 4.329
IPC2_peptide 4.495
IPC2.peptide.svr19 4.445
Protein with the highest isoelectric point:
>tr|A0A4D5ZMI8|A0A4D5ZMI8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan353 OX=2558654 GN=JavanS353_0005 PE=4 SV=1
MM1 pKa = 7.98 IITQSQAKK9 pKa = 9.46 ALRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.98 KK15 pKa = 10.76 ADD17 pKa = 3.54 LQLKK21 pKa = 9.54 NYY23 pKa = 9.21 EE24 pKa = 4.01 LAFEE28 pKa = 4.77 IGVSPKK34 pKa = 9.47 TVPKK38 pKa = 10.16 ILKK41 pKa = 9.83 GDD43 pKa = 3.5 YY44 pKa = 9.18 KK45 pKa = 10.76 APKK48 pKa = 9.3 RR49 pKa = 11.84 IYY51 pKa = 10.12 ASVMEE56 pKa = 4.73 WLAKK60 pKa = 10.37 DD61 pKa = 3.6 YY62 pKa = 11.54
Molecular weight: 7.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.037
IPC2_protein 9.355
IPC_protein 9.282
Toseland 10.087
ProMoST 9.721
Dawson 10.248
Bjellqvist 9.867
Wikipedia 10.394
Rodwell 10.877
Grimsley 10.306
Solomon 10.277
Lehninger 10.248
Nozaki 10.043
DTASelect 9.867
Thurlkill 10.101
EMBOSS 10.467
Sillero 10.16
Patrickios 10.643
IPC_peptide 10.277
IPC2_peptide 8.302
IPC2.peptide.svr19 8.369
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
3100
53
388
172.2
20.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.065 ± 0.484
0.742 ± 0.152
6.29 ± 0.437
7.806 ± 0.823
5.032 ± 0.65
4.129 ± 0.506
1.645 ± 0.283
7.194 ± 0.558
11.387 ± 0.533
9.903 ± 0.323
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.226 ± 0.262
6.355 ± 0.476
2.032 ± 0.286
3.935 ± 0.294
4.258 ± 0.237
6.29 ± 0.382
5.968 ± 0.684
5.419 ± 0.358
0.71 ± 0.118
4.613 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here