Gordonia phage JuJu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KR23|A0A516KR23_9CAUD Uncharacterized protein OS=Gordonia phage JuJu OX=2590929 GN=25 PE=4 SV=1
MM1 pKa = 8.03ADD3 pKa = 3.04TTFTIEE9 pKa = 4.17EE10 pKa = 4.39FPGVTFTVLVGSGGLPGLPSNSMRR34 pKa = 11.84LVASVVNPWYY44 pKa = 10.18EE45 pKa = 3.6PEE47 pKa = 3.95YY48 pKa = 10.96DD49 pKa = 4.2YY50 pKa = 11.99GLDD53 pKa = 3.48PNGYY57 pKa = 7.75TEE59 pKa = 6.35LSDD62 pKa = 2.66RR63 pKa = 11.84WKK65 pKa = 10.68RR66 pKa = 11.84HH67 pKa = 3.69TQFLEE72 pKa = 4.43VINMGFLGPAGAPVPTEE89 pKa = 3.9PPPPVSDD96 pKa = 3.5PTPIAEE102 pKa = 4.47EE103 pKa = 4.17PTDD106 pKa = 3.65GG107 pKa = 4.75

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KR53|A0A516KR53_9CAUD Uncharacterized protein OS=Gordonia phage JuJu OX=2590929 GN=53 PE=4 SV=1
MM1 pKa = 7.38NATATEE7 pKa = 4.13QPTVDD12 pKa = 3.56TYY14 pKa = 11.53RR15 pKa = 11.84DD16 pKa = 3.43PTEE19 pKa = 3.88FEE21 pKa = 4.1KK22 pKa = 11.06AVLNGLQRR30 pKa = 11.84RR31 pKa = 11.84HH32 pKa = 6.08FQFQWGFGGEE42 pKa = 4.13GSIPQVYY49 pKa = 8.82MGYY52 pKa = 8.81TDD54 pKa = 3.9DD55 pKa = 5.68QVIVWADD62 pKa = 3.33GTEE65 pKa = 4.04EE66 pKa = 4.79FIPDD70 pKa = 3.24PRR72 pKa = 11.84IARR75 pKa = 11.84TEE77 pKa = 3.51RR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84ARR82 pKa = 11.84NRR84 pKa = 11.84AGRR87 pKa = 11.84KK88 pKa = 6.79SRR90 pKa = 11.84RR91 pKa = 11.84INRR94 pKa = 11.84IRR96 pKa = 11.84ARR98 pKa = 11.84RR99 pKa = 3.47

Molecular weight: 11.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

17478

37

1839

198.6

21.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.46 ± 0.492

0.95 ± 0.158

7.135 ± 0.382

5.927 ± 0.341

2.746 ± 0.126

8.227 ± 0.385

2.3 ± 0.178

4.423 ± 0.157

3.347 ± 0.205

7.547 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.177

2.969 ± 0.177

5.727 ± 0.251

3.507 ± 0.158

7.827 ± 0.427

5.075 ± 0.174

6.688 ± 0.205

7.627 ± 0.235

2.111 ± 0.112

2.18 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski