Mycobacterium phage Ramen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TML6|A0A5J6TML6_9CAUD Uncharacterized protein OS=Mycobacterium phage Ramen OX=2599876 GN=76 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 6.62RR5 pKa = 11.84CVGDD9 pKa = 3.24DD10 pKa = 5.08CGICAQRR17 pKa = 11.84IEE19 pKa = 4.15QAEE22 pKa = 4.04YY23 pKa = 11.16DD24 pKa = 4.19RR25 pKa = 11.84DD26 pKa = 4.25CPADD30 pKa = 4.77DD31 pKa = 4.06YY32 pKa = 11.62PDD34 pKa = 4.44YY35 pKa = 11.65YY36 pKa = 11.28DD37 pKa = 3.5GTT39 pKa = 4.14

Molecular weight: 4.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TKY7|A0A5J6TKY7_9CAUD Uncharacterized protein OS=Mycobacterium phage Ramen OX=2599876 GN=88 PE=4 SV=1
MM1 pKa = 7.4SNIIAAAAPAAGRR14 pKa = 11.84FNATAALNLILGINLTDD31 pKa = 3.63GQKK34 pKa = 10.2RR35 pKa = 11.84ARR37 pKa = 11.84LLALAVSNDD46 pKa = 3.35AACEE50 pKa = 3.89FNLRR54 pKa = 11.84AGRR57 pKa = 11.84KK58 pKa = 8.39AVAAGRR64 pKa = 11.84LSQASEE70 pKa = 4.03YY71 pKa = 11.26ADD73 pKa = 3.16AAEE76 pKa = 4.78FYY78 pKa = 10.58NNRR81 pKa = 11.84AARR84 pKa = 11.84LRR86 pKa = 11.84AEE88 pKa = 3.88ARR90 pKa = 11.84AII92 pKa = 3.67

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

18626

23

1278

194.0

21.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.095 ± 0.502

1.122 ± 0.141

6.582 ± 0.262

5.804 ± 0.243

2.647 ± 0.148

8.928 ± 0.431

2.131 ± 0.175

3.747 ± 0.167

3.431 ± 0.187

8.241 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.096

2.727 ± 0.165

5.702 ± 0.24

3.382 ± 0.121

7.114 ± 0.347

5.009 ± 0.17

5.9 ± 0.188

7.903 ± 0.237

2.11 ± 0.101

2.276 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski