Amino acid dipepetide frequency for Peptoniphilus harei ACS-146-V-Sch2b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.847AlaAla: 3.847 ± 0.114	0.63AlaCys: 0.63 ± 0.037	3.12AlaAsp: 3.12 ± 0.089	3.75AlaGlu: 3.75 ± 0.105	2.624AlaPhe: 2.624 ± 0.086	4.3AlaGly: 4.3 ± 0.121	0.822AlaHis: 0.822 ± 0.037	5.344AlaIle: 5.344 ± 0.109	5.41AlaLys: 5.41 ± 0.125	6.051AlaLeu: 6.051 ± 0.136	1.688AlaMet: 1.688 ± 0.061	2.858AlaAsn: 2.858 ± 0.082	1.566AlaPro: 1.566 ± 0.064	1.365AlaGln: 1.365 ± 0.054	2.571AlaArg: 2.571 ± 0.081	3.669AlaSer: 3.669 ± 0.083	2.736AlaThr: 2.736 ± 0.081	3.676AlaVal: 3.676 ± 0.094	0.286AlaTrp: 0.286 ± 0.025	2.34AlaTyr: 2.34 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	0.437CysAla: 0.437 ± 0.03	0.097CysCys: 0.097 ± 0.014	0.6CysAsp: 0.6 ± 0.032	0.492CysGlu: 0.492 ± 0.029	0.297CysPhe: 0.297 ± 0.024	0.908CysGly: 0.908 ± 0.048	0.18CysHis: 0.18 ± 0.022	0.574CysIle: 0.574 ± 0.032	0.589CysLys: 0.589 ± 0.037	0.688CysLeu: 0.688 ± 0.032	0.169CysMet: 0.169 ± 0.018	0.376CysAsn: 0.376 ± 0.028	0.393CysPro: 0.393 ± 0.032	0.228CysGln: 0.228 ± 0.021	0.195CysArg: 0.195 ± 0.017	0.485CysSer: 0.485 ± 0.029	0.301CysThr: 0.301 ± 0.025	0.453CysVal: 0.453 ± 0.03	0.05CysTrp: 0.05 ± 0.009	0.261CysTyr: 0.261 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	3.241AspAla: 3.241 ± 0.092	0.428AspCys: 0.428 ± 0.035	3.595AspAsp: 3.595 ± 0.09	5.895AspGlu: 5.895 ± 0.129	3.942AspPhe: 3.942 ± 0.097	3.983AspGly: 3.983 ± 0.087	0.925AspHis: 0.925 ± 0.04	5.831AspIle: 5.831 ± 0.121	6.759AspLys: 6.759 ± 0.115	7.048AspLeu: 7.048 ± 0.148	1.507AspMet: 1.507 ± 0.05	2.944AspAsn: 2.944 ± 0.078	1.866AspPro: 1.866 ± 0.06	1.327AspGln: 1.327 ± 0.052	2.478AspArg: 2.478 ± 0.071	3.37AspSer: 3.37 ± 0.076	2.579AspThr: 2.579 ± 0.068	3.896AspVal: 3.896 ± 0.088	0.351AspTrp: 0.351 ± 0.027	3.309AspTyr: 3.309 ± 0.087	0.0AspXaa: 0.0 ± 0.0
Glu	4.731GluAla: 4.731 ± 0.116	0.439GluCys: 0.439 ± 0.033	6.214GluAsp: 6.214 ± 0.127	8.534GluGlu: 8.534 ± 0.156	3.208GluPhe: 3.208 ± 0.067	4.364GluGly: 4.364 ± 0.095	0.721GluHis: 0.721 ± 0.038	8.108GluIle: 8.108 ± 0.155	8.986GluLys: 8.986 ± 0.152	6.391GluLeu: 6.391 ± 0.124	2.094GluMet: 2.094 ± 0.064	5.623GluAsn: 5.623 ± 0.132	1.32GluPro: 1.32 ± 0.052	1.165GluGln: 1.165 ± 0.058	3.157GluArg: 3.157 ± 0.088	3.75GluSer: 3.75 ± 0.107	3.074GluThr: 3.074 ± 0.076	5.763GluVal: 5.763 ± 0.127	0.329GluTrp: 0.329 ± 0.023	2.661GluTyr: 2.661 ± 0.074	0.0GluXaa: 0.0 ± 0.0
Phe	2.746PheAla: 2.746 ± 0.078	0.406PheCys: 0.406 ± 0.028	3.243PheAsp: 3.243 ± 0.077	3.311PheGlu: 3.311 ± 0.076	2.206PhePhe: 2.206 ± 0.082	3.124PheGly: 3.124 ± 0.095	0.556PheHis: 0.556 ± 0.034	4.309PheIle: 4.309 ± 0.124	4.144PheLys: 4.144 ± 0.077	4.596PheLeu: 4.596 ± 0.12	1.202PheMet: 1.202 ± 0.052	2.435PheAsn: 2.435 ± 0.072	1.151PhePro: 1.151 ± 0.05	0.936PheGln: 0.936 ± 0.045	1.474PheArg: 1.474 ± 0.052	3.199PheSer: 3.199 ± 0.082	2.386PheThr: 2.386 ± 0.069	3.065PheVal: 3.065 ± 0.071	0.283PheTrp: 0.283 ± 0.022	2.145PheTyr: 2.145 ± 0.067	0.0PheXaa: 0.0 ± 0.0
Gly	4.236GlyAla: 4.236 ± 0.116	0.567GlyCys: 0.567 ± 0.037	3.735GlyAsp: 3.735 ± 0.094	4.708GlyGlu: 4.708 ± 0.117	3.118GlyPhe: 3.118 ± 0.083	4.452GlyGly: 4.452 ± 0.128	1.057GlyHis: 1.057 ± 0.048	6.095GlyIle: 6.095 ± 0.105	5.822GlyLys: 5.822 ± 0.099	6.422GlyLeu: 6.422 ± 0.134	1.705GlyMet: 1.705 ± 0.061	2.92GlyAsn: 2.92 ± 0.085	1.376GlyPro: 1.376 ± 0.055	1.637GlyGln: 1.637 ± 0.057	2.687GlyArg: 2.687 ± 0.072	3.801GlySer: 3.801 ± 0.09	3.072GlyThr: 3.072 ± 0.083	4.274GlyVal: 4.274 ± 0.104	0.378GlyTrp: 0.378 ± 0.029	2.841GlyTyr: 2.841 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	0.774HisAla: 0.774 ± 0.044	0.139HisCys: 0.139 ± 0.015	0.817HisAsp: 0.817 ± 0.041	0.949HisGlu: 0.949 ± 0.051	0.628HisPhe: 0.628 ± 0.034	0.949HisGly: 0.949 ± 0.042	0.255HisHis: 0.255 ± 0.024	1.099HisIle: 1.099 ± 0.051	0.995HisLys: 0.995 ± 0.041	1.096HisLeu: 1.096 ± 0.043	0.318HisMet: 0.318 ± 0.024	0.571HisAsn: 0.571 ± 0.031	0.607HisPro: 0.607 ± 0.039	0.323HisGln: 0.323 ± 0.023	0.503HisArg: 0.503 ± 0.038	0.765HisSer: 0.765 ± 0.037	0.617HisThr: 0.617 ± 0.031	0.677HisVal: 0.677 ± 0.036	0.106HisTrp: 0.106 ± 0.015	0.569HisTyr: 0.569 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.495IleAla: 5.495 ± 0.125	0.868IleCys: 0.868 ± 0.041	5.97IleAsp: 5.97 ± 0.114	7.172IleGlu: 7.172 ± 0.142	4.616IlePhe: 4.616 ± 0.133	5.759IleGly: 5.759 ± 0.117	1.066IleHis: 1.066 ± 0.041	7.934IleIle: 7.934 ± 0.168	7.864IleLys: 7.864 ± 0.114	9.153IleLeu: 9.153 ± 0.192	1.901IleMet: 1.901 ± 0.064	4.915IleAsn: 4.915 ± 0.1	2.874IlePro: 2.874 ± 0.075	1.615IleGln: 1.615 ± 0.052	3.171IleArg: 3.171 ± 0.084	6.691IleSer: 6.691 ± 0.123	3.917IleThr: 3.917 ± 0.1	5.418IleVal: 5.418 ± 0.116	0.418IleTrp: 0.418 ± 0.027	3.735IleTyr: 3.735 ± 0.085	0.0IleXaa: 0.0 ± 0.0
Lys	5.021LysAla: 5.021 ± 0.119	0.45LysCys: 0.45 ± 0.033	7.402LysAsp: 7.402 ± 0.129	8.931LysGlu: 8.931 ± 0.161	3.667LysPhe: 3.667 ± 0.094	4.831LysGly: 4.831 ± 0.096	0.822LysHis: 0.822 ± 0.036	9.132LysIle: 9.132 ± 0.162	9.393LysLys: 9.393 ± 0.194	7.991LysLeu: 7.991 ± 0.14	2.434LysMet: 2.434 ± 0.067	7.015LysAsn: 7.015 ± 0.121	1.955LysPro: 1.955 ± 0.078	1.459LysGln: 1.459 ± 0.064	3.5LysArg: 3.5 ± 0.097	5.321LysSer: 5.321 ± 0.111	4.539LysThr: 4.539 ± 0.108	5.818LysVal: 5.818 ± 0.124	0.464LysTrp: 0.464 ± 0.032	3.538LysTyr: 3.538 ± 0.081	0.0LysXaa: 0.0 ± 0.0
Leu	6.161LeuAla: 6.161 ± 0.128	0.701LeuCys: 0.701 ± 0.036	6.346LeuAsp: 6.346 ± 0.123	7.222LeuGlu: 7.222 ± 0.147	4.06LeuPhe: 4.06 ± 0.11	6.512LeuGly: 6.512 ± 0.139	1.028LeuHis: 1.028 ± 0.044	8.211LeuIle: 8.211 ± 0.163	8.942LeuLys: 8.942 ± 0.136	7.995LeuLeu: 7.995 ± 0.151	2.535LeuMet: 2.535 ± 0.08	5.286LeuAsn: 5.286 ± 0.11	2.903LeuPro: 2.903 ± 0.084	1.824LeuGln: 1.824 ± 0.068	3.753LeuArg: 3.753 ± 0.092	6.897LeuSer: 6.897 ± 0.133	4.348LeuThr: 4.348 ± 0.096	6.117LeuVal: 6.117 ± 0.103	0.442LeuTrp: 0.442 ± 0.031	2.979LeuTyr: 2.979 ± 0.074	0.0LeuXaa: 0.0 ± 0.0
Met	1.832MetAla: 1.832 ± 0.065	0.16MetCys: 0.16 ± 0.02	1.672MetAsp: 1.672 ± 0.057	2.011MetGlu: 2.011 ± 0.068	0.802MetPhe: 0.802 ± 0.045	1.925MetGly: 1.925 ± 0.066	0.235MetHis: 0.235 ± 0.019	2.131MetIle: 2.131 ± 0.072	2.714MetLys: 2.714 ± 0.071	2.004MetLeu: 2.004 ± 0.069	0.639MetMet: 0.639 ± 0.036	1.367MetAsn: 1.367 ± 0.055	0.875MetPro: 0.875 ± 0.043	0.543MetGln: 0.543 ± 0.036	0.964MetArg: 0.964 ± 0.044	1.543MetSer: 1.543 ± 0.05	1.461MetThr: 1.461 ± 0.055	1.455MetVal: 1.455 ± 0.054	0.119MetTrp: 0.119 ± 0.015	0.679MetTyr: 0.679 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	2.643AsnAla: 2.643 ± 0.074	0.384AsnCys: 0.384 ± 0.028	2.59AsnAsp: 2.59 ± 0.076	3.621AsnGlu: 3.621 ± 0.098	3.146AsnPhe: 3.146 ± 0.094	2.848AsnGly: 2.848 ± 0.084	0.732AsnHis: 0.732 ± 0.043	5.517AsnIle: 5.517 ± 0.102	5.625AsnLys: 5.625 ± 0.129	6.249AsnLeu: 6.249 ± 0.145	1.277AsnMet: 1.277 ± 0.047	3.015AsnAsn: 3.015 ± 0.122	2.098AsnPro: 2.098 ± 0.075	1.255AsnGln: 1.255 ± 0.053	2.237AsnArg: 2.237 ± 0.072	3.348AsnSer: 3.348 ± 0.094	2.401AsnThr: 2.401 ± 0.082	3.004AsnVal: 3.004 ± 0.091	0.363AsnTrp: 0.363 ± 0.024	2.654AsnTyr: 2.654 ± 0.082	0.0AsnXaa: 0.0 ± 0.0
Pro	1.54ProAla: 1.54 ± 0.062	0.228ProCys: 0.228 ± 0.022	1.646ProAsp: 1.646 ± 0.06	2.336ProGlu: 2.336 ± 0.071	1.252ProPhe: 1.252 ± 0.044	1.912ProGly: 1.912 ± 0.071	0.448ProHis: 0.448 ± 0.024	2.454ProIle: 2.454 ± 0.077	2.424ProLys: 2.424 ± 0.072	2.557ProLeu: 2.557 ± 0.071	0.73ProMet: 0.73 ± 0.035	1.419ProAsn: 1.419 ± 0.051	0.547ProPro: 0.547 ± 0.043	0.8ProGln: 0.8 ± 0.039	1.081ProArg: 1.081 ± 0.043	1.854ProSer: 1.854 ± 0.063	1.402ProThr: 1.402 ± 0.053	1.971ProVal: 1.971 ± 0.068	0.191ProTrp: 0.191 ± 0.016	1.066ProTyr: 1.066 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	1.492GlnAla: 1.492 ± 0.063	0.15GlnCys: 0.15 ± 0.018	1.356GlnAsp: 1.356 ± 0.049	1.914GlnGlu: 1.914 ± 0.06	0.804GlnPhe: 0.804 ± 0.033	1.404GlnGly: 1.404 ± 0.058	0.242GlnHis: 0.242 ± 0.023	1.799GlnIle: 1.799 ± 0.063	1.927GlnLys: 1.927 ± 0.05	1.514GlnLeu: 1.514 ± 0.059	0.582GlnMet: 0.582 ± 0.03	1.164GlnAsn: 1.164 ± 0.058	0.413GlnPro: 0.413 ± 0.03	0.389GlnGln: 0.389 ± 0.029	1.042GlnArg: 1.042 ± 0.05	1.105GlnSer: 1.105 ± 0.048	0.912GlnThr: 0.912 ± 0.048	1.74GlnVal: 1.74 ± 0.057	0.136GlnTrp: 0.136 ± 0.017	0.692GlnTyr: 0.692 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.393ArgAla: 2.393 ± 0.066	0.286ArgCys: 0.286 ± 0.024	3.094ArgAsp: 3.094 ± 0.09	4.133ArgGlu: 4.133 ± 0.101	1.554ArgPhe: 1.554 ± 0.06	2.536ArgGly: 2.536 ± 0.068	0.593ArgHis: 0.593 ± 0.034	3.168ArgIle: 3.168 ± 0.068	3.149ArgLys: 3.149 ± 0.08	3.265ArgLeu: 3.265 ± 0.085	0.967ArgMet: 0.967 ± 0.04	1.982ArgAsn: 1.982 ± 0.055	1.173ArgPro: 1.173 ± 0.052	1.044ArgGln: 1.044 ± 0.046	1.749ArgArg: 1.749 ± 0.064	2.059ArgSer: 2.059 ± 0.074	1.76ArgThr: 1.76 ± 0.069	2.437ArgVal: 2.437 ± 0.07	0.176ArgTrp: 0.176 ± 0.019	1.455ArgTyr: 1.455 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	3.126SerAla: 3.126 ± 0.086	0.527SerCys: 0.527 ± 0.036	3.623SerAsp: 3.623 ± 0.103	4.111SerGlu: 4.111 ± 0.11	3.293SerPhe: 3.293 ± 0.08	4.452SerGly: 4.452 ± 0.103	0.965SerHis: 0.965 ± 0.042	5.585SerIle: 5.585 ± 0.114	5.443SerLys: 5.443 ± 0.121	6.622SerLeu: 6.622 ± 0.13	1.553SerMet: 1.553 ± 0.054	2.995SerAsn: 2.995 ± 0.093	1.81SerPro: 1.81 ± 0.06	1.793SerGln: 1.793 ± 0.06	2.338SerArg: 2.338 ± 0.074	4.195SerSer: 4.195 ± 0.105	2.832SerThr: 2.832 ± 0.071	3.672SerVal: 3.672 ± 0.091	0.413SerTrp: 0.413 ± 0.032	2.841SerTyr: 2.841 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	2.735ThrAla: 2.735 ± 0.086	0.439ThrCys: 0.439 ± 0.028	2.736ThrAsp: 2.736 ± 0.08	3.058ThrGlu: 3.058 ± 0.073	2.112ThrPhe: 2.112 ± 0.065	3.474ThrGly: 3.474 ± 0.082	0.719ThrHis: 0.719 ± 0.04	4.104ThrIle: 4.104 ± 0.087	3.548ThrLys: 3.548 ± 0.087	4.318ThrLeu: 4.318 ± 0.085	1.101ThrMet: 1.101 ± 0.042	2.397ThrAsn: 2.397 ± 0.092	1.611ThrPro: 1.611 ± 0.049	0.868ThrGln: 0.868 ± 0.042	1.949ThrArg: 1.949 ± 0.053	3.232ThrSer: 3.232 ± 0.077	2.226ThrThr: 2.226 ± 0.062	3.091ThrVal: 3.091 ± 0.085	0.259ThrTrp: 0.259 ± 0.024	1.892ThrTyr: 1.892 ± 0.063	0.0ThrXaa: 0.0 ± 0.0
Val	3.861ValAla: 3.861 ± 0.102	0.538ValCys: 0.538 ± 0.034	4.394ValAsp: 4.394 ± 0.092	4.972ValGlu: 4.972 ± 0.111	3.186ValPhe: 3.186 ± 0.092	4.241ValGly: 4.241 ± 0.106	0.749ValHis: 0.749 ± 0.039	5.434ValIle: 5.434 ± 0.112	5.484ValLys: 5.484 ± 0.13	6.066ValLeu: 6.066 ± 0.124	1.593ValMet: 1.593 ± 0.054	3.265ValAsn: 3.265 ± 0.101	1.999ValPro: 1.999 ± 0.063	1.11ValGln: 1.11 ± 0.04	2.323ValArg: 2.323 ± 0.067	4.087ValSer: 4.087 ± 0.095	3.19ValThr: 3.19 ± 0.091	4.241ValVal: 4.241 ± 0.09	0.284ValTrp: 0.284 ± 0.022	2.353ValTyr: 2.353 ± 0.073	0.0ValXaa: 0.0 ± 0.0
Trp	0.413TrpAla: 0.413 ± 0.032	0.04TrpCys: 0.04 ± 0.008	0.382TrpAsp: 0.382 ± 0.031	0.376TrpGlu: 0.376 ± 0.026	0.217TrpPhe: 0.217 ± 0.023	0.384TrpGly: 0.384 ± 0.028	0.094TrpHis: 0.094 ± 0.013	0.464TrpIle: 0.464 ± 0.03	0.455TrpLys: 0.455 ± 0.028	0.387TrpLeu: 0.387 ± 0.03	0.185TrpMet: 0.185 ± 0.019	0.319TrpAsn: 0.319 ± 0.025	0.114TrpPro: 0.114 ± 0.018	0.178TrpGln: 0.178 ± 0.02	0.202TrpArg: 0.202 ± 0.021	0.279TrpSer: 0.279 ± 0.025	0.273TrpThr: 0.273 ± 0.025	0.288TrpVal: 0.288 ± 0.026	0.048TrpTrp: 0.048 ± 0.009	0.226TrpTyr: 0.226 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.931TyrAla: 1.931 ± 0.062	0.297TyrCys: 0.297 ± 0.023	2.736TyrAsp: 2.736 ± 0.08	3.137TyrGlu: 3.137 ± 0.081	2.184TyrPhe: 2.184 ± 0.067	2.544TyrGly: 2.544 ± 0.063	0.547TyrHis: 0.547 ± 0.034	3.259TyrIle: 3.259 ± 0.086	4.008TyrLys: 4.008 ± 0.098	3.85TyrLeu: 3.85 ± 0.104	0.951TyrMet: 0.951 ± 0.047	2.142TyrAsn: 2.142 ± 0.067	1.208TyrPro: 1.208 ± 0.045	0.916TyrGln: 0.916 ± 0.04	1.602TyrArg: 1.602 ± 0.06	2.54TyrSer: 2.54 ± 0.062	1.844TyrThr: 1.844 ± 0.051	2.312TyrVal: 2.312 ± 0.067	0.228TyrTrp: 0.228 ± 0.021	1.855TyrTyr: 1.855 ± 0.08	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1724 proteins (544872 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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