Simian virus 40 (SV40)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03093-2|VP2-2_SV40 Isoform of P03093 Isoform VP3 of Minor capsid protein VP2 OS=Simian virus 40 OX=1891767 PE=1 SV=3
MM1 pKa = 7.91DD2 pKa = 4.22KK3 pKa = 10.85VLNRR7 pKa = 11.84EE8 pKa = 3.87EE9 pKa = 4.53SLQLMDD15 pKa = 5.07LLGLEE20 pKa = 4.32RR21 pKa = 11.84SAWGNIPLMRR31 pKa = 11.84KK32 pKa = 9.63AYY34 pKa = 10.0LKK36 pKa = 10.14KK37 pKa = 10.51CKK39 pKa = 9.6EE40 pKa = 3.81FHH42 pKa = 6.71PDD44 pKa = 3.01KK45 pKa = 11.45GGDD48 pKa = 3.56EE49 pKa = 4.2EE50 pKa = 5.76KK51 pKa = 9.91MKK53 pKa = 10.96KK54 pKa = 9.54MNTLYY59 pKa = 10.92KK60 pKa = 10.61KK61 pKa = 9.97MEE63 pKa = 5.06DD64 pKa = 3.58GVKK67 pKa = 10.15YY68 pKa = 10.46AHH70 pKa = 6.27QPDD73 pKa = 4.58FGGFWDD79 pKa = 3.66ATEE82 pKa = 4.06IPTYY86 pKa = 10.0GTDD89 pKa = 4.08EE90 pKa = 4.03WEE92 pKa = 4.27QWWNAFNEE100 pKa = 4.27EE101 pKa = 4.09NLFCSEE107 pKa = 4.65EE108 pKa = 4.2MPSSDD113 pKa = 5.63DD114 pKa = 3.38EE115 pKa = 4.27ATADD119 pKa = 4.13SQHH122 pKa = 5.69STPPKK127 pKa = 9.69KK128 pKa = 9.86KK129 pKa = 10.24RR130 pKa = 11.84KK131 pKa = 9.58ALLTT135 pKa = 3.92

Molecular weight: 15.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03093-3|VP2-3_SV40 Isoform of P03093 Isoform VP4 of Minor capsid protein VP2 OS=Simian virus 40 OX=1891767 PE=1 SV=3
MM1 pKa = 6.9VRR3 pKa = 11.84QVANRR8 pKa = 11.84EE9 pKa = 3.81GLQISFGHH17 pKa = 6.33TYY19 pKa = 11.43DD20 pKa = 5.02NIDD23 pKa = 3.6EE24 pKa = 4.76ADD26 pKa = 4.19SIQQVTEE33 pKa = 3.44RR34 pKa = 11.84WEE36 pKa = 4.19AQSQSPNVQSGEE48 pKa = 4.11FIEE51 pKa = 5.28KK52 pKa = 10.46FEE54 pKa = 4.64APGGANQRR62 pKa = 11.84TAPQWMLPLLLGLYY76 pKa = 10.49GSVTSALKK84 pKa = 10.39AYY86 pKa = 10.1EE87 pKa = 4.67DD88 pKa = 4.14GPNKK92 pKa = 10.07KK93 pKa = 9.46KK94 pKa = 10.58RR95 pKa = 11.84KK96 pKa = 8.59LSRR99 pKa = 11.84GSSQKK104 pKa = 9.55TKK106 pKa = 9.52GTSASAKK113 pKa = 9.36ARR115 pKa = 11.84HH116 pKa = 5.54KK117 pKa = 10.52RR118 pKa = 11.84RR119 pKa = 11.84NRR121 pKa = 11.84SSRR124 pKa = 11.84SS125 pKa = 3.28

Molecular weight: 13.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

3

9

2175

23

708

241.7

27.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.805 ± 1.134

1.655 ± 0.539

5.793 ± 0.482

7.08 ± 0.605

3.954 ± 0.367

6.069 ± 0.496

1.931 ± 0.26

3.816 ± 0.475

7.402 ± 1.041

9.149 ± 0.603

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.759 ± 0.489

4.276 ± 0.391

5.195 ± 0.608

5.057 ± 0.551

5.425 ± 0.972

7.034 ± 0.699

5.655 ± 0.752

5.471 ± 0.736

1.931 ± 0.29

3.54 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski