Dubosiella newyorkensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Dubosiella

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7NK53|A0A1U7NK53_9FIRM YhcH/YjgK/YiaL family protein OS=Dubosiella newyorkensis OX=1862672 GN=BO225_10845 PE=4 SV=1
MM1 pKa = 7.61SLLQIQGVQDD11 pKa = 3.47YY12 pKa = 10.35EE13 pKa = 5.03RR14 pKa = 11.84IVHH17 pKa = 6.7EE18 pKa = 4.28YY19 pKa = 9.07PNVVVEE25 pKa = 7.7FFATWCPHH33 pKa = 5.28CQAMQPDD40 pKa = 4.34VEE42 pKa = 4.36QAAEE46 pKa = 3.9QFEE49 pKa = 4.55QVVIAQADD57 pKa = 3.44VDD59 pKa = 4.29QNEE62 pKa = 4.39EE63 pKa = 3.85LCDD66 pKa = 3.54AFNVQGTPTFIFVKK80 pKa = 10.27DD81 pKa = 3.91GKK83 pKa = 9.8PVLDD87 pKa = 3.51TVGQISYY94 pKa = 10.49EE95 pKa = 4.02DD96 pKa = 3.71LVDD99 pKa = 5.34FIQKK103 pKa = 10.47GEE105 pKa = 4.2SKK107 pKa = 10.93

Molecular weight: 12.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7NLI0|A0A1U7NLI0_9FIRM Uncharacterized protein OS=Dubosiella newyorkensis OX=1862672 GN=BO225_08040 PE=4 SV=1
MM1 pKa = 7.69LMPKK5 pKa = 8.22RR6 pKa = 11.84TKK8 pKa = 9.71YY9 pKa = 10.1RR10 pKa = 11.84RR11 pKa = 11.84PHH13 pKa = 5.6RR14 pKa = 11.84LSYY17 pKa = 10.34EE18 pKa = 4.05GRR20 pKa = 11.84SKK22 pKa = 11.03AGRR25 pKa = 11.84SVDD28 pKa = 3.8FGEE31 pKa = 4.39YY32 pKa = 10.76GLVAEE37 pKa = 4.7SGNYY41 pKa = 8.93VSNRR45 pKa = 11.84QIEE48 pKa = 4.24AARR51 pKa = 11.84IAMTRR56 pKa = 11.84YY57 pKa = 7.46MKK59 pKa = 10.53RR60 pKa = 11.84GGKK63 pKa = 8.23VWIRR67 pKa = 11.84IFPHH71 pKa = 4.87MARR74 pKa = 11.84TKK76 pKa = 10.47KK77 pKa = 10.0PLEE80 pKa = 3.82VRR82 pKa = 11.84MGSGKK87 pKa = 10.1GAPEE91 pKa = 3.67GWVAVVKK98 pKa = 10.04PGRR101 pKa = 11.84VMFEE105 pKa = 3.64VGGVSEE111 pKa = 4.41EE112 pKa = 4.22VARR115 pKa = 11.84EE116 pKa = 4.03ALRR119 pKa = 11.84LAAHH123 pKa = 7.36KK124 pKa = 10.68LPVKK128 pKa = 9.85TRR130 pKa = 11.84FVRR133 pKa = 11.84KK134 pKa = 10.32GEE136 pKa = 4.09VEE138 pKa = 3.87

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2225

0

2225

664121

38

4566

298.5

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.671 ± 0.057

1.26 ± 0.019

5.718 ± 0.042

7.511 ± 0.063

4.465 ± 0.041

6.172 ± 0.044

2.083 ± 0.021

7.901 ± 0.046

7.483 ± 0.047

9.746 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.885 ± 0.031

4.529 ± 0.036

3.476 ± 0.025

4.023 ± 0.039

3.902 ± 0.038

6.119 ± 0.037

4.861 ± 0.047

6.156 ± 0.043

0.986 ± 0.019

4.051 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski