Mucilaginibacter gossypii
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G8N9U1|A0A1G8N9U1_9SPHI Pimeloyl-ACP methyl ester carboxylesterase OS=Mucilaginibacter gossypii OX=551996 GN=SAMN05192573_13210 PE=4 SV=1
MM1 pKa = 7.94 PDD3 pKa = 3.23 WEE5 pKa = 4.67 TFGPPFQHH13 pKa = 6.92 RR14 pKa = 11.84 LISTTSIATEE24 pKa = 4.18 VFFDD28 pKa = 4.51 LYY30 pKa = 10.89 FSNSVTYY37 pKa = 10.65 DD38 pKa = 3.32 SADD41 pKa = 3.43 SPLLL45 pKa = 3.82
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.786
IPC2_protein 3.973
IPC_protein 3.757
Toseland 3.579
ProMoST 3.986
Dawson 3.783
Bjellqvist 3.961
Wikipedia 3.783
Rodwell 3.617
Grimsley 3.516
Solomon 3.732
Lehninger 3.694
Nozaki 3.948
DTASelect 4.126
Thurlkill 3.694
EMBOSS 3.783
Sillero 3.897
Patrickios 1.914
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A1G8KNW6|A0A1G8KNW6_9SPHI Uncharacterized protein OS=Mucilaginibacter gossypii OX=551996 GN=SAMN05192573_12124 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.29 KK3 pKa = 10.13 IILILCLAVAGITNANAQTVIVMRR27 pKa = 11.84 PVRR30 pKa = 11.84 RR31 pKa = 11.84 VVVTPAHH38 pKa = 4.95 TVIVRR43 pKa = 11.84 PVRR46 pKa = 11.84 RR47 pKa = 11.84 VVVARR52 pKa = 11.84 PVVVTRR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 VVVVHH65 pKa = 6.37 HH66 pKa = 6.79
Molecular weight: 7.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.392
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.945
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6105
0
6105
2079586
25
3000
340.6
38.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.72 ± 0.031
0.789 ± 0.009
5.451 ± 0.023
5.239 ± 0.031
4.895 ± 0.023
6.939 ± 0.028
1.942 ± 0.012
7.198 ± 0.027
6.979 ± 0.027
9.387 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.254 ± 0.017
5.705 ± 0.03
3.97 ± 0.018
3.77 ± 0.021
3.869 ± 0.018
6.199 ± 0.021
5.847 ± 0.024
6.38 ± 0.022
1.224 ± 0.013
4.243 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here