Wuhan insect virus 8
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KK77|A0A1L3KK77_9VIRU Uncharacterized protein OS=Wuhan insect virus 8 OX=1923739 PE=4 SV=1
MM1 pKa = 7.31 SSSYY5 pKa = 10.71 FITLFSLILLSLYY18 pKa = 10.25 LCKK21 pKa = 10.6 NFQNVSSSQLLSTFYY36 pKa = 11.12 NSSFWNKK43 pKa = 9.85 LGRR46 pKa = 11.84 SASISNHH53 pKa = 6.75 PINEE57 pKa = 4.03 YY58 pKa = 10.74 LSAKK62 pKa = 9.98 ARR64 pKa = 11.84 LDD66 pKa = 3.51 LLGTYY71 pKa = 9.85 SSSVFSFDD79 pKa = 3.61 RR80 pKa = 11.84 EE81 pKa = 4.36 CISVVKK87 pKa = 9.72 TNALLEE93 pKa = 4.29 VDD95 pKa = 3.99 CQLPSRR101 pKa = 11.84 CGTLHH106 pKa = 6.95 HH107 pKa = 6.92 KK108 pKa = 10.33 LLSKK112 pKa = 9.44 TFYY115 pKa = 8.35 GQEE118 pKa = 3.65 VRR120 pKa = 11.84 VCYY123 pKa = 10.29 SFHH126 pKa = 6.68 HH127 pKa = 6.06 SVYY130 pKa = 10.99 ADD132 pKa = 4.66 AINLYY137 pKa = 8.45 TIKK140 pKa = 10.46 FPRR143 pKa = 11.84 DD144 pKa = 3.09 LHH146 pKa = 7.88 YY147 pKa = 11.54 GMLTQHH153 pKa = 7.07 DD154 pKa = 4.75 SVDD157 pKa = 3.29 VHH159 pKa = 7.29 DD160 pKa = 4.82 IVPVDD165 pKa = 3.98 FFEE168 pKa = 6.13 HH169 pKa = 6.21 IVIANATDD177 pKa = 5.17 GYY179 pKa = 11.13 HH180 pKa = 7.31 LFPQICLQQYY190 pKa = 8.3 TSYY193 pKa = 11.54 SDD195 pKa = 4.7 DD196 pKa = 4.1 LPPSVVFKK204 pKa = 11.01 EE205 pKa = 4.34 SDD207 pKa = 3.27 DD208 pKa = 4.0 TLCVSHH214 pKa = 6.96 TSLANVDD221 pKa = 3.76 CRR223 pKa = 11.84 PLFFPSVAAIVTTFDD238 pKa = 3.15 FPIAFKK244 pKa = 10.83 YY245 pKa = 10.24 NPYY248 pKa = 10.89 NYY250 pKa = 10.22 LLHH253 pKa = 6.93 LKK255 pKa = 9.8 FRR257 pKa = 11.84 NQDD260 pKa = 3.23 PDD262 pKa = 3.64 VFGYY266 pKa = 10.75 AADD269 pKa = 3.69 TTRR272 pKa = 11.84 GYY274 pKa = 11.06 FPISFINNLNYY285 pKa = 10.01 VSRR288 pKa = 11.84 SPLSSHH294 pKa = 6.71 FLDD297 pKa = 4.18 RR298 pKa = 11.84 NGSSTVFSRR307 pKa = 11.84 NYY309 pKa = 9.34 IYY311 pKa = 10.57 FDD313 pKa = 3.51 SRR315 pKa = 11.84 VHH317 pKa = 5.95 SFSSKK322 pKa = 9.77 DD323 pKa = 3.12 YY324 pKa = 9.96 CYY326 pKa = 10.43 RR327 pKa = 11.84 IEE329 pKa = 4.35 SLYY332 pKa = 10.92 KK333 pKa = 10.52 NPISVLASTLISVVSPIFYY352 pKa = 10.49 EE353 pKa = 4.06 LLEE356 pKa = 4.1 LLEE359 pKa = 4.81 IEE361 pKa = 4.35 VEE363 pKa = 4.13 NIIRR367 pKa = 11.84 LIVKK371 pKa = 10.17 AFLFFLKK378 pKa = 10.6 LVVEE382 pKa = 4.46 LLSLIITPNVLSALMCAFSIYY403 pKa = 10.01 IYY405 pKa = 10.76 CFDD408 pKa = 3.86 FVITFVSFLIFLAFFLSYY426 pKa = 11.19
Molecular weight: 48.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.558
IPC2_protein 5.664
IPC_protein 5.728
Toseland 6.148
ProMoST 6.122
Dawson 6.046
Bjellqvist 6.033
Wikipedia 6.059
Rodwell 6.033
Grimsley 6.287
Solomon 6.046
Lehninger 6.033
Nozaki 6.326
DTASelect 6.517
Thurlkill 6.532
EMBOSS 6.504
Sillero 6.427
Patrickios 0.693
IPC_peptide 6.059
IPC2_peptide 6.427
IPC2.peptide.svr19 6.282
Protein with the highest isoelectric point:
>tr|A0A1L3KKD6|A0A1L3KKD6_9VIRU Uncharacterized protein OS=Wuhan insect virus 8 OX=1923739 PE=4 SV=1
MM1 pKa = 7.65 ASSEE5 pKa = 4.46 SNIGKK10 pKa = 9.99 RR11 pKa = 11.84 SGNRR15 pKa = 11.84 AARR18 pKa = 11.84 RR19 pKa = 11.84 SRR21 pKa = 11.84 NLPRR25 pKa = 11.84 NSTVPAVVDD34 pKa = 3.61 EE35 pKa = 4.42 LTPEE39 pKa = 3.87 VSGVLRR45 pKa = 11.84 SGSGRR50 pKa = 11.84 RR51 pKa = 11.84 NVNGIKK57 pKa = 10.26 SKK59 pKa = 10.95 NKK61 pKa = 10.19 SYY63 pKa = 11.35 DD64 pKa = 3.44 FNQVFSDD71 pKa = 3.28 IALTLTSAISNPLVLLTIALSVGVITTHH99 pKa = 6.9 NFPQNKK105 pKa = 9.01 GFIYY109 pKa = 9.2 DD110 pKa = 3.87 TFRR113 pKa = 11.84 EE114 pKa = 4.23 KK115 pKa = 11.15 NDD117 pKa = 3.94 TISRR121 pKa = 11.84 WVVDD125 pKa = 3.43 NGQKK129 pKa = 10.12 VAGFSIFLPAVVDD142 pKa = 3.7 SPRR145 pKa = 11.84 NFRR148 pKa = 11.84 SVIALTSFLWVMVIPEE164 pKa = 4.3 SSVVEE169 pKa = 4.14 YY170 pKa = 10.0 FLQALAVHH178 pKa = 6.63 TYY180 pKa = 10.2 FRR182 pKa = 11.84 LKK184 pKa = 9.76 TDD186 pKa = 3.24 NSRR189 pKa = 11.84 LTLLAIIGIAWFLGYY204 pKa = 8.88 FHH206 pKa = 7.6 IPTTKK211 pKa = 10.49
Molecular weight: 23.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 9.575
IPC_protein 10.043
Toseland 10.409
ProMoST 10.116
Dawson 10.555
Bjellqvist 10.218
Wikipedia 10.73
Rodwell 10.877
Grimsley 10.613
Solomon 10.613
Lehninger 10.584
Nozaki 10.379
DTASelect 10.218
Thurlkill 10.423
EMBOSS 10.804
Sillero 10.467
Patrickios 10.599
IPC_peptide 10.613
IPC2_peptide 8.843
IPC2.peptide.svr19 8.771
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3187
211
2550
1062.3
121.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.801 ± 0.372
2.604 ± 0.595
6.15 ± 0.71
4.518 ± 0.791
6.056 ± 1.135
3.483 ± 0.502
3.044 ± 0.334
6.37 ± 0.358
5.083 ± 0.76
9.162 ± 1.171
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.008 ± 0.627
5.742 ± 0.305
3.671 ± 0.217
2.479 ± 0.229
5.24 ± 0.555
9.727 ± 1.222
5.491 ± 0.337
8.033 ± 0.395
0.565 ± 0.171
5.773 ± 0.679
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here