Wuhan insect virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KK77|A0A1L3KK77_9VIRU Uncharacterized protein OS=Wuhan insect virus 8 OX=1923739 PE=4 SV=1
MM1 pKa = 7.31SSSYY5 pKa = 10.71FITLFSLILLSLYY18 pKa = 10.25LCKK21 pKa = 10.6NFQNVSSSQLLSTFYY36 pKa = 11.12NSSFWNKK43 pKa = 9.85LGRR46 pKa = 11.84SASISNHH53 pKa = 6.75PINEE57 pKa = 4.03YY58 pKa = 10.74LSAKK62 pKa = 9.98ARR64 pKa = 11.84LDD66 pKa = 3.51LLGTYY71 pKa = 9.85SSSVFSFDD79 pKa = 3.61RR80 pKa = 11.84EE81 pKa = 4.36CISVVKK87 pKa = 9.72TNALLEE93 pKa = 4.29VDD95 pKa = 3.99CQLPSRR101 pKa = 11.84CGTLHH106 pKa = 6.95HH107 pKa = 6.92KK108 pKa = 10.33LLSKK112 pKa = 9.44TFYY115 pKa = 8.35GQEE118 pKa = 3.65VRR120 pKa = 11.84VCYY123 pKa = 10.29SFHH126 pKa = 6.68HH127 pKa = 6.06SVYY130 pKa = 10.99ADD132 pKa = 4.66AINLYY137 pKa = 8.45TIKK140 pKa = 10.46FPRR143 pKa = 11.84DD144 pKa = 3.09LHH146 pKa = 7.88YY147 pKa = 11.54GMLTQHH153 pKa = 7.07DD154 pKa = 4.75SVDD157 pKa = 3.29VHH159 pKa = 7.29DD160 pKa = 4.82IVPVDD165 pKa = 3.98FFEE168 pKa = 6.13HH169 pKa = 6.21IVIANATDD177 pKa = 5.17GYY179 pKa = 11.13HH180 pKa = 7.31LFPQICLQQYY190 pKa = 8.3TSYY193 pKa = 11.54SDD195 pKa = 4.7DD196 pKa = 4.1LPPSVVFKK204 pKa = 11.01EE205 pKa = 4.34SDD207 pKa = 3.27DD208 pKa = 4.0TLCVSHH214 pKa = 6.96TSLANVDD221 pKa = 3.76CRR223 pKa = 11.84PLFFPSVAAIVTTFDD238 pKa = 3.15FPIAFKK244 pKa = 10.83YY245 pKa = 10.24NPYY248 pKa = 10.89NYY250 pKa = 10.22LLHH253 pKa = 6.93LKK255 pKa = 9.8FRR257 pKa = 11.84NQDD260 pKa = 3.23PDD262 pKa = 3.64VFGYY266 pKa = 10.75AADD269 pKa = 3.69TTRR272 pKa = 11.84GYY274 pKa = 11.06FPISFINNLNYY285 pKa = 10.01VSRR288 pKa = 11.84SPLSSHH294 pKa = 6.71FLDD297 pKa = 4.18RR298 pKa = 11.84NGSSTVFSRR307 pKa = 11.84NYY309 pKa = 9.34IYY311 pKa = 10.57FDD313 pKa = 3.51SRR315 pKa = 11.84VHH317 pKa = 5.95SFSSKK322 pKa = 9.77DD323 pKa = 3.12YY324 pKa = 9.96CYY326 pKa = 10.43RR327 pKa = 11.84IEE329 pKa = 4.35SLYY332 pKa = 10.92KK333 pKa = 10.52NPISVLASTLISVVSPIFYY352 pKa = 10.49EE353 pKa = 4.06LLEE356 pKa = 4.1LLEE359 pKa = 4.81IEE361 pKa = 4.35VEE363 pKa = 4.13NIIRR367 pKa = 11.84LIVKK371 pKa = 10.17AFLFFLKK378 pKa = 10.6LVVEE382 pKa = 4.46LLSLIITPNVLSALMCAFSIYY403 pKa = 10.01IYY405 pKa = 10.76CFDD408 pKa = 3.86FVITFVSFLIFLAFFLSYY426 pKa = 11.19

Molecular weight:
48.88 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KKD6|A0A1L3KKD6_9VIRU Uncharacterized protein OS=Wuhan insect virus 8 OX=1923739 PE=4 SV=1
MM1 pKa = 7.65ASSEE5 pKa = 4.46SNIGKK10 pKa = 9.99RR11 pKa = 11.84SGNRR15 pKa = 11.84AARR18 pKa = 11.84RR19 pKa = 11.84SRR21 pKa = 11.84NLPRR25 pKa = 11.84NSTVPAVVDD34 pKa = 3.61EE35 pKa = 4.42LTPEE39 pKa = 3.87VSGVLRR45 pKa = 11.84SGSGRR50 pKa = 11.84RR51 pKa = 11.84NVNGIKK57 pKa = 10.26SKK59 pKa = 10.95NKK61 pKa = 10.19SYY63 pKa = 11.35DD64 pKa = 3.44FNQVFSDD71 pKa = 3.28IALTLTSAISNPLVLLTIALSVGVITTHH99 pKa = 6.9NFPQNKK105 pKa = 9.01GFIYY109 pKa = 9.2DD110 pKa = 3.87TFRR113 pKa = 11.84EE114 pKa = 4.23KK115 pKa = 11.15NDD117 pKa = 3.94TISRR121 pKa = 11.84WVVDD125 pKa = 3.43NGQKK129 pKa = 10.12VAGFSIFLPAVVDD142 pKa = 3.7SPRR145 pKa = 11.84NFRR148 pKa = 11.84SVIALTSFLWVMVIPEE164 pKa = 4.3SSVVEE169 pKa = 4.14YY170 pKa = 10.0FLQALAVHH178 pKa = 6.63TYY180 pKa = 10.2FRR182 pKa = 11.84LKK184 pKa = 9.76TDD186 pKa = 3.24NSRR189 pKa = 11.84LTLLAIIGIAWFLGYY204 pKa = 8.88FHH206 pKa = 7.6IPTTKK211 pKa = 10.49

Molecular weight:
23.4 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3187

211

2550

1062.3

121.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.801 ± 0.372

2.604 ± 0.595

6.15 ± 0.71

4.518 ± 0.791

6.056 ± 1.135

3.483 ± 0.502

3.044 ± 0.334

6.37 ± 0.358

5.083 ± 0.76

9.162 ± 1.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.008 ± 0.627

5.742 ± 0.305

3.671 ± 0.217

2.479 ± 0.229

5.24 ± 0.555

9.727 ± 1.222

5.491 ± 0.337

8.033 ± 0.395

0.565 ± 0.171

5.773 ± 0.679

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski