Streptococcus satellite phage Javan248
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHS3|A0A4D5ZHS3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan248 OX=2558590 GN=JavanS248_0014 PE=4 SV=1
MM1 pKa = 7.42 VDD3 pKa = 4.25 KK4 pKa = 11.03 ISDD7 pKa = 3.44 KK8 pKa = 11.28 QEE10 pKa = 3.84 NKK12 pKa = 10.2 KK13 pKa = 10.4 FADD16 pKa = 3.92 LADD19 pKa = 3.64 MADD22 pKa = 3.36 ALKK25 pKa = 10.65 RR26 pKa = 11.84 GIDD29 pKa = 3.62 LQSIAFDD36 pKa = 4.44 AIVNIEE42 pKa = 4.14 DD43 pKa = 3.6 DD44 pKa = 4.45 SIRR47 pKa = 11.84 SLAAIKK53 pKa = 10.17 ALNVAYY59 pKa = 10.08 DD60 pKa = 3.63 YY61 pKa = 11.48 NQYY64 pKa = 11.07 LSNQLQVLHH73 pKa = 7.21 DD74 pKa = 3.95 SLYY77 pKa = 11.02 EE78 pKa = 4.0 EE79 pKa = 4.73 VV80 pKa = 4.17
Molecular weight: 9.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.389
IPC2_protein 4.279
IPC_protein 4.202
Toseland 3.986
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.151
Rodwell 4.024
Grimsley 3.897
Solomon 4.19
Lehninger 4.139
Nozaki 4.317
DTASelect 4.584
Thurlkill 4.05
EMBOSS 4.151
Sillero 4.329
Patrickios 3.859
IPC_peptide 4.19
IPC2_peptide 4.304
IPC2.peptide.svr19 4.269
Protein with the highest isoelectric point:
>tr|A0A4D5ZHV0|A0A4D5ZHV0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan248 OX=2558590 GN=JavanS248_0013 PE=4 SV=1
MM1 pKa = 8.0 LITQTQARR9 pKa = 11.84 AIRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.62 QADD17 pKa = 3.4 KK18 pKa = 11.27 KK19 pKa = 10.16 LTAKK23 pKa = 10.18 QAGEE27 pKa = 4.37 EE28 pKa = 3.88 IGITQVTYY36 pKa = 10.82 RR37 pKa = 11.84 KK38 pKa = 9.56 IRR40 pKa = 11.84 DD41 pKa = 3.57 GGNVKK46 pKa = 10.33 PGVYY50 pKa = 9.37 QKK52 pKa = 11.53 AMEE55 pKa = 4.32 WLAKK59 pKa = 10.35 DD60 pKa = 3.63 YY61 pKa = 11.54
Molecular weight: 6.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.139
IPC2_protein 9.502
IPC_protein 9.56
Toseland 10.321
ProMoST 9.926
Dawson 10.452
Bjellqvist 10.072
Wikipedia 10.584
Rodwell 11.038
Grimsley 10.496
Solomon 10.482
Lehninger 10.467
Nozaki 10.277
DTASelect 10.072
Thurlkill 10.321
EMBOSS 10.701
Sillero 10.365
Patrickios 10.804
IPC_peptide 10.496
IPC2_peptide 8.536
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
3834
58
1290
225.5
25.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.025 ± 2.4
0.391 ± 0.184
5.738 ± 0.832
5.712 ± 1.323
4.251 ± 0.389
6.338 ± 1.534
1.043 ± 0.253
7.538 ± 0.442
8.477 ± 0.996
9.911 ± 0.68
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.052 ± 0.339
5.582 ± 0.699
1.93 ± 0.248
4.512 ± 0.292
3.547 ± 0.541
6.729 ± 0.675
5.66 ± 0.579
6.182 ± 0.805
0.835 ± 0.215
3.547 ± 0.92
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here