Listeria phage LP-125
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 189 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4U6T7|S4U6T7_9CAUD Uncharacterized protein OS=Listeria phage LP-125 OX=1173766 GN=LP125_047 PE=4 SV=1
MM1 pKa = 8.12 DD2 pKa = 5.13 LNKK5 pKa = 10.05 FLTEE9 pKa = 3.76 VDD11 pKa = 4.15 KK12 pKa = 11.09 VTSAYY17 pKa = 10.54 GEE19 pKa = 4.07 DD20 pKa = 3.4 ANYY23 pKa = 9.69 EE24 pKa = 3.62 ISFIRR29 pKa = 11.84 EE30 pKa = 3.53 KK31 pKa = 11.13 YY32 pKa = 10.37 ADD34 pKa = 4.07 LDD36 pKa = 3.79 EE37 pKa = 5.05 GAKK40 pKa = 9.62 QDD42 pKa = 4.56 CEE44 pKa = 3.94 EE45 pKa = 4.01 TDD47 pKa = 3.63 FFEE50 pKa = 4.19 EE51 pKa = 4.18 VEE53 pKa = 4.92 YY54 pKa = 10.45 ISQMQTGEE62 pKa = 4.24 DD63 pKa = 3.76 CFEE66 pKa = 4.19 GYY68 pKa = 9.86 IFRR71 pKa = 11.84 KK72 pKa = 9.49 IKK74 pKa = 9.12 NTDD77 pKa = 2.29 WCLCVTYY84 pKa = 10.5 AII86 pKa = 4.8
Molecular weight: 10.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.805
IPC2_protein 4.037
IPC_protein 3.961
Toseland 3.77
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.795
Grimsley 3.694
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.19
Thurlkill 3.808
EMBOSS 3.834
Sillero 4.075
Patrickios 0.947
IPC_peptide 3.91
IPC2_peptide 4.05
IPC2.peptide.svr19 3.957
Protein with the highest isoelectric point:
>tr|S4UCD2|S4UCD2_9CAUD Uncharacterized protein OS=Listeria phage LP-125 OX=1173766 GN=LP125_171 PE=4 SV=1
MM1 pKa = 7.77 ANNTRR6 pKa = 11.84 GNLPRR11 pKa = 11.84 GYY13 pKa = 8.15 TALGDD18 pKa = 3.61 GSEE21 pKa = 4.12 SHH23 pKa = 5.94 KK24 pKa = 9.73 TVEE27 pKa = 3.95 RR28 pKa = 11.84 ALRR31 pKa = 11.84 GYY33 pKa = 8.57 YY34 pKa = 9.3 TVDD37 pKa = 3.97 FSNIYY42 pKa = 9.91 EE43 pKa = 4.34 HH44 pKa = 5.52 MTEE47 pKa = 3.98 RR48 pKa = 11.84 DD49 pKa = 3.36 LEE51 pKa = 4.31 VRR53 pKa = 11.84 QAYY56 pKa = 10.09 AKK58 pKa = 10.56 DD59 pKa = 4.33 LISQRR64 pKa = 11.84 TGIPLDD70 pKa = 3.56 MILFRR75 pKa = 11.84 KK76 pKa = 8.42 KK77 pKa = 8.62 TEE79 pKa = 4.01 KK80 pKa = 9.25 QTLTAIDD87 pKa = 3.42 EE88 pKa = 4.4 VFYY91 pKa = 11.14 VKK93 pKa = 10.5 QGVKK97 pKa = 9.87 TIGKK101 pKa = 9.59 VSIRR105 pKa = 11.84 SQRR108 pKa = 11.84 LLSSNMLIIIYY119 pKa = 8.63 MSKK122 pKa = 9.08 SHH124 pKa = 6.15 MPRR127 pKa = 11.84 VVRR130 pKa = 11.84 NTGMKK135 pKa = 9.78 TYY137 pKa = 9.47 TKK139 pKa = 10.09 RR140 pKa = 11.84 SKK142 pKa = 11.08 SDD144 pKa = 2.68 IRR146 pKa = 11.84 VAKK149 pKa = 9.98 RR150 pKa = 11.84 QRR152 pKa = 11.84 KK153 pKa = 3.95 TTKK156 pKa = 8.87 RR157 pKa = 11.84 QSYY160 pKa = 9.68
Molecular weight: 18.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 9.838
IPC_protein 10.365
Toseland 10.555
ProMoST 10.204
Dawson 10.687
Bjellqvist 10.35
Wikipedia 10.862
Rodwell 11.082
Grimsley 10.745
Solomon 10.73
Lehninger 10.701
Nozaki 10.511
DTASelect 10.35
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.599
Patrickios 10.774
IPC_peptide 10.745
IPC2_peptide 9.019
IPC2.peptide.svr19 8.7
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
189
0
189
39824
38
1309
210.7
23.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.758 ± 0.227
0.648 ± 0.059
5.951 ± 0.112
8.231 ± 0.288
3.927 ± 0.116
5.868 ± 0.287
1.487 ± 0.088
6.639 ± 0.222
8.651 ± 0.258
8.578 ± 0.219
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.443 ± 0.09
5.672 ± 0.117
2.85 ± 0.146
3.362 ± 0.218
4.103 ± 0.101
6.807 ± 0.178
6.31 ± 0.169
7.237 ± 0.165
0.977 ± 0.065
4.5 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here