Mesocricetus auratus (Golden hamster)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria;

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31698 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q0D8P1|A0A3Q0D8P1_MESAU mesothelin OS=Mesocricetus auratus OX=10036 GN=Msln PE=3 SV=1
MM1 pKa = 7.88LYY3 pKa = 10.11FQEE6 pKa = 4.86RR7 pKa = 11.84SPFPTDD13 pKa = 2.77RR14 pKa = 11.84VGVGRR19 pKa = 11.84HH20 pKa = 4.64MNPYY24 pKa = 10.29VIVNDD29 pKa = 3.6EE30 pKa = 4.1GLVPEE35 pKa = 5.21EE36 pKa = 4.14YY37 pKa = 10.64VSLIPEE43 pKa = 3.88EE44 pKa = 4.42MIPVALEE51 pKa = 3.95AEE53 pKa = 4.41MEE55 pKa = 4.05EE56 pKa = 4.3SLEE59 pKa = 4.09GEE61 pKa = 4.31EE62 pKa = 5.01VIWSHH67 pKa = 5.88VPNEE71 pKa = 4.07EE72 pKa = 4.53EE73 pKa = 4.11EE74 pKa = 4.46NQTEE78 pKa = 4.42TQVEE82 pKa = 4.4NCDD85 pKa = 3.42CTHH88 pKa = 6.35NVEE91 pKa = 5.17EE92 pKa = 5.8DD93 pKa = 4.37DD94 pKa = 6.62DD95 pKa = 7.1DD96 pKa = 7.63DD97 pKa = 7.7DD98 pKa = 7.59DD99 pKa = 7.21DD100 pKa = 6.96DD101 pKa = 4.0EE102 pKa = 7.01ANYY105 pKa = 10.74KK106 pKa = 8.34HH107 pKa = 6.19TEE109 pKa = 3.88NFTGSVNDD117 pKa = 3.51HH118 pKa = 6.41TYY120 pKa = 10.95EE121 pKa = 4.18CTHH124 pKa = 7.23DD125 pKa = 3.73EE126 pKa = 4.26TKK128 pKa = 10.65EE129 pKa = 3.51AAFRR133 pKa = 11.84GKK135 pKa = 10.29DD136 pKa = 3.31EE137 pKa = 4.67NEE139 pKa = 4.08NEE141 pKa = 4.15NQEE144 pKa = 4.19VEE146 pKa = 5.33DD147 pKa = 5.67DD148 pKa = 3.71INPNN152 pKa = 3.24

Molecular weight: 17.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U8CRC8|A0A1U8CRC8_MESAU COMM domain-containing protein 6 isoform X1 OS=Mesocricetus auratus OX=10036 GN=Commd6 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 10.05TGNKK34 pKa = 8.79IRR36 pKa = 3.91

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20469

11229

31698

20061817

18

33063

632.9

70.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.0 ± 0.013

2.15 ± 0.013

4.863 ± 0.008

7.127 ± 0.019

3.492 ± 0.01

6.433 ± 0.018

2.614 ± 0.007

4.181 ± 0.012

5.63 ± 0.016

9.913 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.006

3.493 ± 0.009

6.478 ± 0.025

4.919 ± 0.015

5.732 ± 0.013

8.697 ± 0.018

5.4 ± 0.01

6.012 ± 0.011

1.141 ± 0.005

2.554 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski