Pseudomonas indica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8U1M2|A0A1G8U1M2_9PSED Phosphate-specific transport system accessory protein PhoU OS=Pseudomonas indica OX=137658 GN=SAMN05216186_101522 PE=3 SV=1
MM1 pKa = 7.32HH2 pKa = 7.16THH4 pKa = 6.3QLLQSLPRR12 pKa = 11.84CLLSAALVAGMASTAGVYY30 pKa = 10.17AVDD33 pKa = 4.37GAPPGLFEE41 pKa = 6.68LDD43 pKa = 3.42GNTVDD48 pKa = 5.0GAAAGDD54 pKa = 3.82DD55 pKa = 3.71WGSLHH60 pKa = 7.12LGNQAGSPVAFTGILSDD77 pKa = 3.88PAPVTIYY84 pKa = 9.67TQGGSKK90 pKa = 10.34DD91 pKa = 3.94VNDD94 pKa = 3.58VTQWRR99 pKa = 11.84YY100 pKa = 8.04TNGSVPDD107 pKa = 4.25KK108 pKa = 11.35DD109 pKa = 5.71DD110 pKa = 3.1ITNAYY115 pKa = 7.15ATAYY119 pKa = 9.52INPADD124 pKa = 3.82VSHH127 pKa = 6.82NGQVVHH133 pKa = 6.84AAGDD137 pKa = 3.96LIIYY141 pKa = 9.66FGLDD145 pKa = 2.55RR146 pKa = 11.84FANNGDD152 pKa = 3.32AFAGFWFFQDD162 pKa = 3.73NVTLGANGRR171 pKa = 11.84FNGQHH176 pKa = 5.6VARR179 pKa = 11.84QGMEE183 pKa = 3.9GQPGYY188 pKa = 11.31VPGDD192 pKa = 3.42LLVLVEE198 pKa = 4.33YY199 pKa = 9.55PQGANAQPEE208 pKa = 4.43IKK210 pKa = 10.43VYY212 pKa = 10.22EE213 pKa = 4.32WDD215 pKa = 4.47PLDD218 pKa = 5.09LDD220 pKa = 4.53HH221 pKa = 8.09DD222 pKa = 4.33NVAPNLDD229 pKa = 3.39QRR231 pKa = 11.84YY232 pKa = 9.18SSAAAKK238 pKa = 10.44CDD240 pKa = 3.33GSGNKK245 pKa = 8.66LACAITNVANLPGAPEE261 pKa = 3.77WPYY264 pKa = 10.01TPKK267 pKa = 10.8NGFAGIPFEE276 pKa = 4.59SFFEE280 pKa = 4.27GGINVSKK287 pKa = 10.9LLGGDD292 pKa = 3.68VPCFSSFLAEE302 pKa = 4.17TRR304 pKa = 11.84SSRR307 pKa = 11.84SEE309 pKa = 3.78TSQLKK314 pKa = 10.83DD315 pKa = 3.52FVLGDD320 pKa = 3.95FNLCSIAVTKK330 pKa = 10.62QCTAAVDD337 pKa = 3.65AGDD340 pKa = 4.24GGNSVLVNYY349 pKa = 10.51SGTVTNDD356 pKa = 2.7GGLPLFDD363 pKa = 3.5VTVTDD368 pKa = 5.9DD369 pKa = 3.2MGTANTADD377 pKa = 3.63DD378 pKa = 3.95VVVFGPATLQPGEE391 pKa = 4.22SQPYY395 pKa = 7.63NGSYY399 pKa = 8.85STLSIPATDD408 pKa = 3.36EE409 pKa = 3.91VTAVGHH415 pKa = 6.44RR416 pKa = 11.84NGTSVEE422 pKa = 4.05ATAEE426 pKa = 4.34ASCSPDD432 pKa = 3.21VTPALAVDD440 pKa = 4.54KK441 pKa = 11.2ACTANIASGGAGIDD455 pKa = 3.29VLFNGTVTNTGNVALEE471 pKa = 4.0NVMVVDD477 pKa = 5.49DD478 pKa = 5.18GGTPGDD484 pKa = 3.73TSDD487 pKa = 4.36DD488 pKa = 3.86VTVLGPVTLNAGEE501 pKa = 4.17SMPYY505 pKa = 9.87NGSFSASGSNSSTDD519 pKa = 3.15TVTATGSDD527 pKa = 3.85VLTQSPVEE535 pKa = 4.03ATAQATCEE543 pKa = 4.05ADD545 pKa = 3.3VTPAIEE551 pKa = 4.2VTKK554 pKa = 10.67QCSANINTGGTGIDD568 pKa = 3.32VLFTGTVTNTGDD580 pKa = 3.25VALGNVTVVDD590 pKa = 4.43DD591 pKa = 4.55NGTPGDD597 pKa = 3.76TSDD600 pKa = 4.36DD601 pKa = 3.85VTVLGPISLAPGEE614 pKa = 4.26SANYY618 pKa = 9.5NGSFSASGSSSTDD631 pKa = 2.84TVTASGADD639 pKa = 3.59VLTQSPVEE647 pKa = 4.12AQASASCSADD657 pKa = 3.04VTPAIEE663 pKa = 4.21VTKK666 pKa = 10.74QCTDD670 pKa = 2.93APAFGQAILFDD681 pKa = 3.82GTVTNTGNVALLNVTVVDD699 pKa = 4.68DD700 pKa = 4.4NGTPGDD706 pKa = 3.81TSDD709 pKa = 5.61DD710 pKa = 3.62VTFNLGDD717 pKa = 4.13LAPGAAANYY726 pKa = 9.03NGAYY730 pKa = 8.14TPSLAGSYY738 pKa = 9.71TNTVVATAEE747 pKa = 4.19DD748 pKa = 4.14AVEE751 pKa = 4.07SSPVSSSADD760 pKa = 3.35ATCEE764 pKa = 3.99VPPPPEE770 pKa = 5.48GEE772 pKa = 4.16GCTPGFWKK780 pKa = 10.55NSPGSWGPTGYY791 pKa = 10.55SPNQTVGSVFSLPSGQLANQLGGNSLIQALNYY823 pKa = 10.69AGGNNLLGSAQILLRR838 pKa = 11.84AAVAAVLNASHH849 pKa = 7.71PDD851 pKa = 2.81VDD853 pKa = 4.28YY854 pKa = 10.37PRR856 pKa = 11.84TAASVIADD864 pKa = 3.58VNAALATKK872 pKa = 10.43DD873 pKa = 3.17RR874 pKa = 11.84ATILALAAALDD885 pKa = 3.9ADD887 pKa = 4.3NNLGCDD893 pKa = 3.99LPNDD897 pKa = 3.53NSFF900 pKa = 3.53

Molecular weight: 91.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9LRJ8|A0A1G9LRJ8_9PSED Uncharacterized protein (Fragment) OS=Pseudomonas indica OX=137658 GN=SAMN05216186_1261 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.31RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5180

0

5180

1715093

25

14206

331.1

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.203 ± 0.04

1.001 ± 0.013

5.534 ± 0.037

6.195 ± 0.036

3.578 ± 0.022

8.152 ± 0.041

2.234 ± 0.019

4.442 ± 0.024

3.128 ± 0.029

12.166 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.018

2.806 ± 0.022

5.079 ± 0.026

4.271 ± 0.025

7.168 ± 0.035

5.448 ± 0.033

4.6 ± 0.029

6.889 ± 0.03

1.458 ± 0.015

2.52 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski