Mycobacterium phage Bubbles123

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8DU93|A0A1P8DU93_9CAUD Uncharacterized protein OS=Mycobacterium phage Bubbles123 OX=1932895 GN=SEA_BUBBLES123_91 PE=4 SV=1
MM1 pKa = 7.52NNPEE5 pKa = 3.95LRR7 pKa = 11.84AVLTEE12 pKa = 4.1ALAGHH17 pKa = 5.59QPEE20 pKa = 4.6NYY22 pKa = 9.94GFNCSGCDD30 pKa = 3.34WEE32 pKa = 4.49PANPAVTDD40 pKa = 3.68AAEE43 pKa = 4.29FAAHH47 pKa = 6.3QLDD50 pKa = 4.17VLDD53 pKa = 4.24TAPGVAVIQLPEE65 pKa = 4.37PYY67 pKa = 10.2FEE69 pKa = 4.21ATGDD73 pKa = 3.49EE74 pKa = 4.62FEE76 pKa = 5.05NGRR79 pKa = 11.84KK80 pKa = 9.73DD81 pKa = 3.63YY82 pKa = 11.6AFGDD86 pKa = 3.64VSVFADD92 pKa = 4.18GEE94 pKa = 4.14IHH96 pKa = 7.38LFGAVWDD103 pKa = 4.15TAAIEE108 pKa = 3.97EE109 pKa = 4.33FAAGILAAVAEE120 pKa = 4.71SKK122 pKa = 10.52RR123 pKa = 11.84AAAAAAVVATGEE135 pKa = 4.19EE136 pKa = 4.15AA137 pKa = 3.78

Molecular weight: 14.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8DU88|A0A1P8DU88_9CAUD Uncharacterized protein OS=Mycobacterium phage Bubbles123 OX=1932895 GN=SEA_BUBBLES123_86 PE=4 SV=1
MM1 pKa = 6.68TQRR4 pKa = 11.84KK5 pKa = 9.5GGFDD9 pKa = 4.35WIRR12 pKa = 11.84STYY15 pKa = 9.77RR16 pKa = 11.84VPAKK20 pKa = 10.02RR21 pKa = 11.84GMRR24 pKa = 11.84VVFDD28 pKa = 3.72GRR30 pKa = 11.84PGRR33 pKa = 11.84ILSVDD38 pKa = 3.4GPYY41 pKa = 11.37LMLHH45 pKa = 6.91LDD47 pKa = 3.96SDD49 pKa = 4.41PKK51 pKa = 10.37NLRR54 pKa = 11.84TRR56 pKa = 11.84VHH58 pKa = 5.46PTWRR62 pKa = 11.84MEE64 pKa = 3.89YY65 pKa = 9.3LTT67 pKa = 4.68

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

18259

39

1223

182.6

19.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.762 ± 0.433

1.232 ± 0.141

6.589 ± 0.2

5.833 ± 0.325

3.089 ± 0.193

8.845 ± 0.532

2.35 ± 0.199

4.425 ± 0.189

3.198 ± 0.173

7.246 ± 0.17

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.126

3.489 ± 0.164

6.15 ± 0.221

3.565 ± 0.179

6.709 ± 0.365

5.844 ± 0.261

6.698 ± 0.262

6.966 ± 0.28

2.322 ± 0.149

2.437 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski