Clostridium liquoris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2785 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0B177|A0A2T0B177_9CLOT 1 4-alpha-glucan branching enzyme OS=Clostridium liquoris OX=1289519 GN=CLLI_23770 PE=3 SV=1
MM1 pKa = 7.77ISDD4 pKa = 3.55KK5 pKa = 11.73DD6 pKa = 3.8EE7 pKa = 4.52VIKK10 pKa = 10.66LAEE13 pKa = 4.94EE14 pKa = 4.22ILSSLQINGYY24 pKa = 9.68NIEE27 pKa = 4.0QMTPEE32 pKa = 4.2EE33 pKa = 4.05VSKK36 pKa = 11.45VVDD39 pKa = 4.19EE40 pKa = 4.29YY41 pKa = 11.79LKK43 pKa = 10.72DD44 pKa = 3.62VYY46 pKa = 11.29EE47 pKa = 4.88DD48 pKa = 4.13FVDD51 pKa = 5.01GYY53 pKa = 11.62DD54 pKa = 5.56DD55 pKa = 5.05IFSEE59 pKa = 4.74YY60 pKa = 9.13NTSCEE65 pKa = 4.13SFEE68 pKa = 4.48DD69 pKa = 4.99FYY71 pKa = 11.88SGFTKK76 pKa = 10.64NIKK79 pKa = 10.43NKK81 pKa = 9.99DD82 pKa = 3.18IHH84 pKa = 7.77IEE86 pKa = 4.05SYY88 pKa = 11.46LNN90 pKa = 3.38

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0B8Y5|A0A2T0B8Y5_9CLOT Ribosome-associated protein OS=Clostridium liquoris OX=1289519 GN=CLLI_03860 PE=4 SV=1
MM1 pKa = 7.46FMTYY5 pKa = 8.42QPKK8 pKa = 9.63KK9 pKa = 7.83RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.69RR21 pKa = 11.84MKK23 pKa = 10.05SLAGRR28 pKa = 11.84NVLKK32 pKa = 10.56RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2785

0

2785

831833

31

2884

298.7

33.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.707 ± 0.055

1.247 ± 0.02

5.454 ± 0.044

7.376 ± 0.066

4.317 ± 0.043

6.578 ± 0.039

1.373 ± 0.018

10.01 ± 0.051

9.376 ± 0.053

8.922 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.026

6.247 ± 0.051

2.942 ± 0.025

2.335 ± 0.024

3.463 ± 0.033

6.027 ± 0.033

4.738 ± 0.031

6.313 ± 0.042

0.674 ± 0.015

4.167 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski