Thermogemmatispora tikiterensis
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4652 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A328VE27|A0A328VE27_9CHLR HTH arsR-type domain-containing protein OS=Thermogemmatispora tikiterensis OX=1825093 GN=A4R35_10300 PE=4 SV=1
MM1 pKa = 7.61 AWLDD5 pKa = 3.48 STSRR9 pKa = 11.84 CGYY12 pKa = 7.74 TDD14 pKa = 3.14 SVSIYY19 pKa = 10.55 CRR21 pKa = 11.84 VLLQKK26 pKa = 10.65 DD27 pKa = 3.14 CWLAVDD33 pKa = 4.62 QPLSPARR40 pKa = 11.84 TGSLRR45 pKa = 11.84 GAFADD50 pKa = 4.29 LEE52 pKa = 4.4 EE53 pKa = 4.41 EE54 pKa = 4.32 QMTDD58 pKa = 3.61 SPLTPDD64 pKa = 3.88 PEE66 pKa = 4.29 RR67 pKa = 11.84 FPLPFLLHH75 pKa = 5.51 YY76 pKa = 10.12 LEE78 pKa = 5.04 PVSEE82 pKa = 4.62 RR83 pKa = 11.84 EE84 pKa = 3.85 DD85 pKa = 3.58 DD86 pKa = 3.81 LTISEE91 pKa = 4.67 TPYY94 pKa = 9.37 QTGPDD99 pKa = 4.15 GPTHH103 pKa = 6.62 TDD105 pKa = 3.24 YY106 pKa = 11.73 LDD108 pKa = 4.95 DD109 pKa = 4.3 EE110 pKa = 5.73 DD111 pKa = 4.9 ISGGAPTNTGQQADD125 pKa = 3.88 SSPQEE130 pKa = 4.3 DD131 pKa = 3.76 AVVWWW136 pKa = 3.93
Molecular weight: 15.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A328VIL6|A0A328VIL6_9CHLR Spermine synthase OS=Thermogemmatispora tikiterensis OX=1825093 GN=A4R35_15180 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.45 RR3 pKa = 11.84 TWQPKK8 pKa = 8.26 RR9 pKa = 11.84 IPRR12 pKa = 11.84 KK13 pKa = 9.39 RR14 pKa = 11.84 EE15 pKa = 3.38 HH16 pKa = 6.69 GFLKK20 pKa = 10.62 RR21 pKa = 11.84 MATRR25 pKa = 11.84 AGRR28 pKa = 11.84 RR29 pKa = 11.84 VLKK32 pKa = 10.5 ARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.67 GRR39 pKa = 11.84 WRR41 pKa = 11.84 LTVV44 pKa = 3.0
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.442
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4652
0
4652
1557656
31
2141
334.8
36.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.037 ± 0.043
1.071 ± 0.013
4.085 ± 0.024
6.539 ± 0.048
3.221 ± 0.02
7.713 ± 0.035
2.237 ± 0.016
4.644 ± 0.028
2.057 ± 0.025
12.711 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.813 ± 0.013
2.223 ± 0.023
5.893 ± 0.036
4.979 ± 0.034
7.725 ± 0.045
5.829 ± 0.033
5.087 ± 0.03
6.808 ± 0.032
1.527 ± 0.017
2.801 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here