Gymnopilus dilepis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Cortinariaceae; Gymnopilus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A409WD23|A0A409WD23_9AGAR Uncharacterized protein OS=Gymnopilus dilepis OX=231916 GN=CVT26_013071 PE=4 SV=1
MM1 pKa = 7.39SAFSFEE7 pKa = 4.51SQHH10 pKa = 5.93SAATIDD16 pKa = 4.04IEE18 pKa = 4.82HH19 pKa = 6.91NPHH22 pKa = 7.54DD23 pKa = 5.07MIDD26 pKa = 3.96SIAEE30 pKa = 3.7HH31 pKa = 7.2DD32 pKa = 4.2LAEE35 pKa = 4.23HH36 pKa = 6.26HH37 pKa = 6.75HH38 pKa = 6.77AEE40 pKa = 4.41STDD43 pKa = 3.55EE44 pKa = 4.59LDD46 pKa = 3.83INTDD50 pKa = 2.99EE51 pKa = 5.24DD52 pKa = 3.91LAEE55 pKa = 4.47ALAVMHH61 pKa = 6.69LLVPDD66 pKa = 4.82FDD68 pKa = 5.69DD69 pKa = 5.04SDD71 pKa = 4.3MIHH74 pKa = 6.25LTGDD78 pKa = 3.41ALEE81 pKa = 4.92VIDD84 pKa = 6.54DD85 pKa = 4.16GVLWDD90 pKa = 3.76QNGPFTGSWDD100 pKa = 3.13VSMNLEE106 pKa = 4.02VLSNIGVEE114 pKa = 4.19AGTFQYY120 pKa = 10.84SFDD123 pKa = 3.43MFYY126 pKa = 10.92SPEE129 pKa = 3.84EE130 pKa = 3.86MAEE133 pKa = 4.05EE134 pKa = 4.33SS135 pKa = 3.52

Molecular weight: 15.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A409Y346|A0A409Y346_9AGAR Uncharacterized protein OS=Gymnopilus dilepis OX=231916 GN=CVT26_006783 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84KK3 pKa = 9.88KK4 pKa = 9.08ITPSEE9 pKa = 4.43QIRR12 pKa = 11.84HH13 pKa = 5.55TNPTAPRR20 pKa = 11.84PHH22 pKa = 6.41KK23 pKa = 9.79RR24 pKa = 11.84AKK26 pKa = 9.85RR27 pKa = 11.84PHH29 pKa = 6.04PAIFGTLSSLSRR41 pKa = 11.84WQLRR45 pKa = 11.84SVAMRR50 pKa = 11.84KK51 pKa = 9.06ILIAGPILSLRR62 pKa = 11.84PIGRR66 pKa = 11.84IVASRR71 pKa = 11.84PPRR74 pKa = 11.84GGIGVTT80 pKa = 3.6

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16218

0

16218

7632278

15

4467

470.6

52.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.222 ± 0.02

1.275 ± 0.007

5.552 ± 0.012

5.912 ± 0.017

3.871 ± 0.012

6.271 ± 0.02

2.57 ± 0.008

4.898 ± 0.015

4.622 ± 0.018

9.456 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.949 ± 0.006

3.542 ± 0.011

6.654 ± 0.022

3.844 ± 0.014

6.189 ± 0.019

9.094 ± 0.026

5.884 ± 0.013

6.141 ± 0.015

1.402 ± 0.007

2.648 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski