Tortoise microvirus 108
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W776|A0A4P8W776_9VIRU Uncharacterized protein OS=Tortoise microvirus 108 OX=2583108 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.36 NALYY6 pKa = 10.43 CLYY9 pKa = 9.15 NTLSTRR15 pKa = 11.84 YY16 pKa = 9.6 GDD18 pKa = 3.94 VVCFPSDD25 pKa = 2.88 AFAVQRR31 pKa = 11.84 IRR33 pKa = 11.84 EE34 pKa = 4.01 NLKK37 pKa = 10.28 PEE39 pKa = 3.81 MLKK42 pKa = 10.51 EE43 pKa = 3.94 IEE45 pKa = 4.23 LCKK48 pKa = 10.11 IGYY51 pKa = 9.42 IDD53 pKa = 4.49 IDD55 pKa = 3.46 TGIAEE60 pKa = 4.08 VHH62 pKa = 5.72 PPIRR66 pKa = 11.84 LSLIVDD72 pKa = 3.67 EE73 pKa = 4.87 TGVPIDD79 pKa = 4.06 NLEE82 pKa = 4.0 KK83 pKa = 10.87
Molecular weight: 9.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.739
IPC2_protein 4.902
IPC_protein 4.711
Toseland 4.571
ProMoST 4.825
Dawson 4.66
Bjellqvist 4.813
Wikipedia 4.533
Rodwell 4.558
Grimsley 4.482
Solomon 4.66
Lehninger 4.609
Nozaki 4.774
DTASelect 4.914
Thurlkill 4.571
EMBOSS 4.558
Sillero 4.825
Patrickios 3.63
IPC_peptide 4.66
IPC2_peptide 4.825
IPC2.peptide.svr19 4.784
Protein with the highest isoelectric point:
>tr|A0A4P8W7D1|A0A4P8W7D1_9VIRU Uncharacterized protein OS=Tortoise microvirus 108 OX=2583108 PE=4 SV=1
MM1 pKa = 6.89 MSSMRR6 pKa = 11.84 SIAPSTRR13 pKa = 11.84 LIGALRR19 pKa = 11.84 DD20 pKa = 3.93 TKK22 pKa = 11.49 SMLVGSWDD30 pKa = 3.82 CLHH33 pKa = 6.31 FTAKK37 pKa = 9.33 TLSRR41 pKa = 11.84 VLFVPRR47 pKa = 11.84 SYY49 pKa = 10.94 RR50 pKa = 11.84 CLMLTLQTVLFFQVAMLLVLMLPFYY75 pKa = 10.36 FLRR78 pKa = 11.84 RR79 pKa = 11.84 LFRR82 pKa = 11.84 RR83 pKa = 11.84 LTIYY87 pKa = 10.86
Molecular weight: 10.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 10.233
IPC_protein 11.403
Toseland 11.462
ProMoST 11.915
Dawson 11.477
Bjellqvist 11.447
Wikipedia 11.93
Rodwell 11.228
Grimsley 11.52
Solomon 11.93
Lehninger 11.828
Nozaki 11.462
DTASelect 11.447
Thurlkill 11.462
EMBOSS 11.945
Sillero 11.462
Patrickios 10.994
IPC_peptide 11.93
IPC2_peptide 10.921
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1797
83
596
224.6
25.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.401 ± 0.95
1.558 ± 0.578
6.01 ± 0.493
4.674 ± 0.933
5.62 ± 0.584
5.954 ± 0.48
1.725 ± 0.373
4.007 ± 0.561
4.285 ± 0.837
9.071 ± 0.858
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.283 ± 0.487
4.786 ± 0.552
4.508 ± 0.514
3.84 ± 1.276
6.177 ± 0.612
10.573 ± 1.038
5.509 ± 0.858
5.287 ± 0.489
0.946 ± 0.191
4.786 ± 0.503
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here