Rubrobacter sp. SCSIO 52915

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Rubrobacteria; Rubrobacterales; Rubrobacteraceae; Rubrobacter; unclassified Rubrobacter

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8PUA9|A0A6G8PUA9_9ACTN Uncharacterized protein OS=Rubrobacter sp. SCSIO 52915 OX=2653852 GN=GBA65_02520 PE=4 SV=1
MM1 pKa = 7.56SGDD4 pKa = 3.61AGDD7 pKa = 4.01DD8 pKa = 3.67KK9 pKa = 11.36IYY11 pKa = 10.83GSDD14 pKa = 3.32GAEE17 pKa = 3.83YY18 pKa = 10.29IADD21 pKa = 3.6HH22 pKa = 6.56NGNDD26 pKa = 3.23RR27 pKa = 11.84LYY29 pKa = 11.25GNGRR33 pKa = 11.84DD34 pKa = 4.77DD35 pKa = 5.2IISANDD41 pKa = 3.59GSASDD46 pKa = 5.31FVDD49 pKa = 3.87CGSGVNDD56 pKa = 3.49SATVDD61 pKa = 3.43KK62 pKa = 11.01YY63 pKa = 11.77VEE65 pKa = 4.18INPDD69 pKa = 2.98GSTLMRR75 pKa = 11.84SDD77 pKa = 3.3TVVNCEE83 pKa = 4.06NIFYY87 pKa = 10.85LL88 pKa = 4.84

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8Q286|A0A6G8Q286_9ACTN Zinc-binding dehydrogenase OS=Rubrobacter sp. SCSIO 52915 OX=2653852 GN=GBA65_01105 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84AKK14 pKa = 8.07THH16 pKa = 5.09GFRR19 pKa = 11.84VRR21 pKa = 11.84MSTVGGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LAVV44 pKa = 3.5

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4019

0

4019

1163057

28

2341

289.4

31.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.605 ± 0.052

0.712 ± 0.01

5.283 ± 0.028

7.973 ± 0.049

3.326 ± 0.024

10.267 ± 0.04

1.76 ± 0.019

3.655 ± 0.028

2.519 ± 0.026

10.18 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.973 ± 0.017

2.135 ± 0.019

5.535 ± 0.035

2.213 ± 0.025

8.422 ± 0.049

5.443 ± 0.028

4.788 ± 0.025

8.513 ± 0.037

1.197 ± 0.014

2.502 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski