Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; Halomonas elongata

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3622 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1V876|E1V876_HALED Uncharacterized protein OS=Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9) OX=768066 GN=HELO_1755 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 9.06KK3 pKa = 8.27TLLATAIAGAMAASGAQAATVYY25 pKa = 10.61NQDD28 pKa = 3.01GTKK31 pKa = 10.39LDD33 pKa = 3.23IYY35 pKa = 11.2GNVQIGFRR43 pKa = 11.84NIEE46 pKa = 3.91AEE48 pKa = 3.87NDD50 pKa = 3.15NGNIEE55 pKa = 4.25TQNDD59 pKa = 3.44VFDD62 pKa = 4.24NGSTIGFAAEE72 pKa = 3.66HH73 pKa = 6.23VIYY76 pKa = 10.73DD77 pKa = 3.83GLTGYY82 pKa = 9.48MKK84 pKa = 10.25IEE86 pKa = 4.19FDD88 pKa = 4.26DD89 pKa = 5.09FKK91 pKa = 11.42ADD93 pKa = 3.42EE94 pKa = 4.51MKK96 pKa = 10.32TAGRR100 pKa = 11.84DD101 pKa = 3.55AGDD104 pKa = 3.3TAYY107 pKa = 10.99VGLKK111 pKa = 10.83GNFGDD116 pKa = 4.31VKK118 pKa = 10.62LGSYY122 pKa = 7.64DD123 pKa = 3.36TLMDD127 pKa = 4.74DD128 pKa = 5.12WIQDD132 pKa = 3.82PITNNEE138 pKa = 3.98YY139 pKa = 10.88FDD141 pKa = 4.29VSDD144 pKa = 3.65TSGSGSSVVAVGGEE158 pKa = 4.17VEE160 pKa = 4.38TDD162 pKa = 2.79QLTYY166 pKa = 10.89VSPSFNGLEE175 pKa = 4.06LAIGTQYY182 pKa = 10.89KK183 pKa = 10.99GDD185 pKa = 3.8MEE187 pKa = 4.5EE188 pKa = 4.38EE189 pKa = 4.39NVTSRR194 pKa = 11.84GNASVFGGAKK204 pKa = 8.05YY205 pKa = 8.26TAGNFSVAATYY216 pKa = 11.17DD217 pKa = 3.3NLDD220 pKa = 3.74NYY222 pKa = 10.59EE223 pKa = 4.08VTQTGVDD230 pKa = 3.42NKK232 pKa = 10.62QEE234 pKa = 3.98FGDD237 pKa = 3.86RR238 pKa = 11.84YY239 pKa = 10.45GVTGQYY245 pKa = 8.27QWNSLRR251 pKa = 11.84VALKK255 pKa = 9.73YY256 pKa = 10.72EE257 pKa = 4.33RR258 pKa = 11.84FDD260 pKa = 3.99SDD262 pKa = 3.81LDD264 pKa = 3.89NVDD267 pKa = 3.35SVNFYY272 pKa = 11.5GLGARR277 pKa = 11.84YY278 pKa = 9.62GYY280 pKa = 10.9GYY282 pKa = 10.72GDD284 pKa = 3.22IYY286 pKa = 11.2GAYY289 pKa = 9.76QYY291 pKa = 11.48VDD293 pKa = 3.13VGGDD297 pKa = 3.23TFGNVVDD304 pKa = 5.22DD305 pKa = 4.25ATSGDD310 pKa = 4.2SPSDD314 pKa = 3.49TASDD318 pKa = 4.38RR319 pKa = 11.84GDD321 pKa = 3.2DD322 pKa = 3.83TYY324 pKa = 11.96NEE326 pKa = 4.44FIIGGTYY333 pKa = 10.26NISDD337 pKa = 3.69AMYY340 pKa = 8.35TWVEE344 pKa = 3.36AAFYY348 pKa = 10.74DD349 pKa = 4.74RR350 pKa = 11.84EE351 pKa = 4.21DD352 pKa = 4.28DD353 pKa = 3.97EE354 pKa = 7.12GDD356 pKa = 3.44GVAAGVTYY364 pKa = 9.67MFF366 pKa = 5.27

Molecular weight: 39.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1VCK1|E1VCK1_HALED DUF461 family protein OS=Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9) OX=768066 GN=HELO_2472 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3622

0

3622

1202401

37

3024

332.0

36.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.184 ± 0.043

0.914 ± 0.012

6.09 ± 0.042

6.72 ± 0.05

3.383 ± 0.026

8.367 ± 0.037

2.47 ± 0.019

4.542 ± 0.036

2.439 ± 0.029

11.382 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.019

2.437 ± 0.021

4.984 ± 0.028

3.614 ± 0.03

7.562 ± 0.046

5.455 ± 0.028

4.988 ± 0.023

7.137 ± 0.034

1.503 ± 0.019

2.312 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski