Sphingobium sp. MI1205

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4473 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126RCP7|A0A126RCP7_9SPHN Uncharacterized protein OS=Sphingobium sp. MI1205 OX=407020 GN=K663_16826 PE=4 SV=1
MM1 pKa = 7.86AKK3 pKa = 8.9LTVVNRR9 pKa = 11.84SGEE12 pKa = 4.16EE13 pKa = 3.79QTIEE17 pKa = 4.08AQDD20 pKa = 3.77GLSVMEE26 pKa = 4.63LLRR29 pKa = 11.84DD30 pKa = 3.58NGFDD34 pKa = 4.18EE35 pKa = 5.85LLALCGGCCSCATCHH50 pKa = 6.46VYY52 pKa = 10.57VDD54 pKa = 3.91PSFADD59 pKa = 3.54SLPTISDD66 pKa = 4.05DD67 pKa = 4.87EE68 pKa = 5.05NDD70 pKa = 4.32LLDD73 pKa = 4.7SSDD76 pKa = 3.35HH77 pKa = 6.39RR78 pKa = 11.84NEE80 pKa = 3.84HH81 pKa = 6.42SRR83 pKa = 11.84LSCQLPFSDD92 pKa = 4.79SLDD95 pKa = 3.54GLRR98 pKa = 11.84VTIAPEE104 pKa = 3.78DD105 pKa = 3.45

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126R433|A0A126R433_9SPHN Chromate transport protein ChrA OS=Sphingobium sp. MI1205 OX=407020 GN=K663_03260 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4473

0

4473

1347481

26

2803

301.2

32.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.816 ± 0.059

0.834 ± 0.012

6.004 ± 0.029

5.499 ± 0.03

3.514 ± 0.023

8.643 ± 0.043

2.072 ± 0.018

5.229 ± 0.023

3.084 ± 0.029

9.968 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.019

2.589 ± 0.023

5.202 ± 0.028

3.299 ± 0.02

7.462 ± 0.04

5.505 ± 0.029

5.066 ± 0.03

6.996 ± 0.026

1.416 ± 0.018

2.224 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski