Murine coronavirus (strain 2) (MHV-2) (Murine hepatitis virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; Murine coronavirus; Murine hepatitis virus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9PYA1|Q9PYA1_CVM2 Non-structural protein 2a OS=Murine coronavirus (strain 2) OX=76344 PE=2 SV=1
MM1 pKa = 7.67AAMQAFADD9 pKa = 4.26KK10 pKa = 9.98PNHH13 pKa = 6.87FINFPLAQFSGVMGKK28 pKa = 7.96YY29 pKa = 10.3LKK31 pKa = 10.59LQSQLVEE38 pKa = 4.08MGLDD42 pKa = 3.76CKK44 pKa = 10.5LQKK47 pKa = 10.45APHH50 pKa = 5.8VSITMLDD57 pKa = 3.25IRR59 pKa = 11.84ADD61 pKa = 3.31QYY63 pKa = 11.5KK64 pKa = 9.51QVEE67 pKa = 4.17FAIQEE72 pKa = 4.43VIDD75 pKa = 5.23DD76 pKa = 3.74MAAYY80 pKa = 9.87EE81 pKa = 4.12GDD83 pKa = 3.31IVFDD87 pKa = 3.88NPHH90 pKa = 5.62MLGRR94 pKa = 11.84CLVLDD99 pKa = 3.42VKK101 pKa = 10.99GFEE104 pKa = 4.45EE105 pKa = 4.03LHH107 pKa = 6.06EE108 pKa = 5.39DD109 pKa = 2.99IVEE112 pKa = 4.05ILRR115 pKa = 11.84KK116 pKa = 9.49RR117 pKa = 11.84GCIADD122 pKa = 4.37QSRR125 pKa = 11.84QWIPHH130 pKa = 4.93CTVAQFEE137 pKa = 4.44EE138 pKa = 4.34EE139 pKa = 4.24KK140 pKa = 10.66EE141 pKa = 4.02INEE144 pKa = 3.8LQFYY148 pKa = 10.15YY149 pKa = 10.98KK150 pKa = 10.7LPFYY154 pKa = 10.68LKK156 pKa = 10.34HH157 pKa = 6.46NNILTDD163 pKa = 3.31ARR165 pKa = 11.84LEE167 pKa = 4.03LVKK170 pKa = 10.53IGSSKK175 pKa = 9.95TDD177 pKa = 2.85GFYY180 pKa = 10.87CSEE183 pKa = 3.82LSIWCGEE190 pKa = 4.08RR191 pKa = 11.84LCYY194 pKa = 10.09KK195 pKa = 10.34PPTPKK200 pKa = 10.32FSDD203 pKa = 2.77IFGYY207 pKa = 10.69CCIDD211 pKa = 4.07KK212 pKa = 10.67IRR214 pKa = 11.84VDD216 pKa = 4.68LEE218 pKa = 4.38IGDD221 pKa = 4.36LPEE224 pKa = 6.18DD225 pKa = 4.99DD226 pKa = 3.96EE227 pKa = 4.58EE228 pKa = 5.02AWAEE232 pKa = 3.96LSYY235 pKa = 10.57HH236 pKa = 4.98YY237 pKa = 10.55QRR239 pKa = 11.84NTYY242 pKa = 8.74FFRR245 pKa = 11.84YY246 pKa = 9.06VHH248 pKa = 7.01DD249 pKa = 3.57NSIYY253 pKa = 9.8FRR255 pKa = 11.84TVCRR259 pKa = 11.84MKK261 pKa = 11.07GCMCC265 pKa = 5.37

Molecular weight: 30.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9PY98|Q9PY98_CVM2 Non-structural protein OS=Murine coronavirus (strain 2) OX=76344 PE=2 SV=1
MM1 pKa = 7.35SFVPGQEE8 pKa = 4.06NAGSRR13 pKa = 11.84SSSGNRR19 pKa = 11.84AGNGILKK26 pKa = 8.59KK27 pKa = 7.37TTWADD32 pKa = 3.05QTEE35 pKa = 4.24RR36 pKa = 11.84GNRR39 pKa = 11.84GRR41 pKa = 11.84RR42 pKa = 11.84NHH44 pKa = 6.79PKK46 pKa = 8.46QTATTQPNAGSVVPHH61 pKa = 5.92YY62 pKa = 10.92SWFSGITQFQKK73 pKa = 10.82GKK75 pKa = 9.99EE76 pKa = 3.93FQFAQGQGVPIASGIPASEE95 pKa = 4.15QKK97 pKa = 10.87GYY99 pKa = 9.69WYY101 pKa = 10.34RR102 pKa = 11.84HH103 pKa = 4.36NRR105 pKa = 11.84RR106 pKa = 11.84SFKK109 pKa = 10.77TPDD112 pKa = 3.54GQHH115 pKa = 5.87KK116 pKa = 10.03QLLPRR121 pKa = 11.84WYY123 pKa = 9.79FYY125 pKa = 11.56YY126 pKa = 10.78LGTGPHH132 pKa = 6.74AGAEE136 pKa = 4.09YY137 pKa = 10.75GDD139 pKa = 5.04DD140 pKa = 3.44IEE142 pKa = 4.76GVVWVASQQADD153 pKa = 3.61TKK155 pKa = 8.29TTADD159 pKa = 3.4VVEE162 pKa = 4.91RR163 pKa = 11.84DD164 pKa = 3.89PSSHH168 pKa = 5.75EE169 pKa = 4.53AIPTRR174 pKa = 11.84FAPGTVLPQGFYY186 pKa = 10.92VEE188 pKa = 4.42GSGRR192 pKa = 11.84SAPASRR198 pKa = 11.84SGSRR202 pKa = 11.84SQSRR206 pKa = 11.84GPNNRR211 pKa = 11.84ARR213 pKa = 11.84SSSNQRR219 pKa = 11.84QPASAVKK226 pKa = 9.82PDD228 pKa = 3.5MAEE231 pKa = 3.97EE232 pKa = 3.7IAALVLAKK240 pKa = 10.33LGKK243 pKa = 10.0DD244 pKa = 2.88AGQPKK249 pKa = 9.61QVTKK253 pKa = 10.71QSAKK257 pKa = 8.91EE258 pKa = 3.89VRR260 pKa = 11.84QKK262 pKa = 10.93ILTKK266 pKa = 9.83PRR268 pKa = 11.84QKK270 pKa = 9.65RR271 pKa = 11.84TPNKK275 pKa = 8.97QCPVQQCFGKK285 pKa = 10.52RR286 pKa = 11.84GPNQNFGGSEE296 pKa = 3.94MLKK299 pKa = 10.6LGTSDD304 pKa = 3.38PQFPILAEE312 pKa = 4.09LAPTPSAFFFGSKK325 pKa = 10.65LEE327 pKa = 4.03LVKK330 pKa = 10.9KK331 pKa = 10.59NSGGADD337 pKa = 3.41EE338 pKa = 4.48PTKK341 pKa = 10.65DD342 pKa = 3.71VYY344 pKa = 10.84EE345 pKa = 4.13LQYY348 pKa = 11.19SGAIRR353 pKa = 11.84FDD355 pKa = 3.3STLPGFEE362 pKa = 4.85TIMKK366 pKa = 10.09VLTEE370 pKa = 4.26NLNAYY375 pKa = 9.23QDD377 pKa = 3.65QAGSVDD383 pKa = 4.3LVSPKK388 pKa = 9.78PPRR391 pKa = 11.84RR392 pKa = 11.84GRR394 pKa = 11.84RR395 pKa = 11.84QAQEE399 pKa = 4.04KK400 pKa = 10.0KK401 pKa = 10.78DD402 pKa = 3.67EE403 pKa = 4.08VDD405 pKa = 3.28NVSVAKK411 pKa = 10.22PKK413 pKa = 10.9SLVQRR418 pKa = 11.84NVSRR422 pKa = 11.84EE423 pKa = 4.02LTPEE427 pKa = 4.02DD428 pKa = 4.12RR429 pKa = 11.84SLLAQILDD437 pKa = 4.15DD438 pKa = 4.33GVVPDD443 pKa = 4.88GLEE446 pKa = 4.2DD447 pKa = 4.13DD448 pKa = 4.85SNVV451 pKa = 3.06

Molecular weight: 49.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

14151

14

7124

1415.1

158.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.918 ± 0.289

3.837 ± 0.379

5.788 ± 0.279

4.056 ± 0.231

5.455 ± 0.166

6.141 ± 0.336

1.661 ± 0.104

4.261 ± 0.506

5.894 ± 0.399

9.264 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.367 ± 0.174

4.897 ± 0.542

3.547 ± 0.417

3.392 ± 0.535

3.611 ± 0.373

7.151 ± 0.45

5.597 ± 0.211

10.105 ± 1.076

1.336 ± 0.085

4.721 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski