Trichoplusia ni granulovirus LBIV-12
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8QL67|A0A1D8QL67_GVTN GIY-YIG domain-containing protein OS=Trichoplusia ni granulovirus LBIV-12 OX=1916701 PE=4 SV=1
MM1 pKa = 7.46 GCDD4 pKa = 3.32 SFITIYY10 pKa = 10.72 FLDD13 pKa = 3.71 EE14 pKa = 4.03 HH15 pKa = 7.2 DD16 pKa = 4.42 KK17 pKa = 11.39 VINKK21 pKa = 10.0 LKK23 pKa = 7.69 MTSYY27 pKa = 10.33 KK28 pKa = 10.17 QRR30 pKa = 11.84 ALSQVITNPPEE41 pKa = 4.08 AKK43 pKa = 10.15 SIQVCFSDD51 pKa = 5.4 DD52 pKa = 3.74 NNEE55 pKa = 4.39 CEE57 pKa = 5.56 CEE59 pKa = 3.99 YY60 pKa = 11.32 CNIDD64 pKa = 6.25 DD65 pKa = 5.04 EE66 pKa = 6.58 DD67 pKa = 4.56 EE68 pKa = 4.84 DD69 pKa = 4.69 DD70 pKa = 3.95
Molecular weight: 8.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.999
IPC_protein 3.935
Toseland 3.732
ProMoST 4.012
Dawson 3.923
Bjellqvist 4.126
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.05
Patrickios 0.896
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.972
Protein with the highest isoelectric point:
>tr|A0A1D8QL90|A0A1D8QL90_GVTN p14.2 OS=Trichoplusia ni granulovirus LBIV-12 OX=1916701 PE=4 SV=1
MM1 pKa = 8.0 DD2 pKa = 4.31 NFDD5 pKa = 3.26 RR6 pKa = 11.84 TEE8 pKa = 3.94 RR9 pKa = 11.84 LRR11 pKa = 11.84 STEE14 pKa = 3.68 RR15 pKa = 11.84 LLRR18 pKa = 11.84 RR19 pKa = 11.84 LIIIYY24 pKa = 10.06 HH25 pKa = 6.12 KK26 pKa = 10.66 LYY28 pKa = 11.04 TNMKK32 pKa = 8.1 KK33 pKa = 10.82 SNVIWQKK40 pKa = 11.05 LFAISLLSTLRR51 pKa = 11.84 FVEE54 pKa = 4.12 RR55 pKa = 11.84 TLKK58 pKa = 10.53 IKK60 pKa = 10.47 ILL62 pKa = 3.78
Molecular weight: 7.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.97
IPC_protein 10.965
Toseland 11.169
ProMoST 11.155
Dawson 11.213
Bjellqvist 10.994
Wikipedia 11.506
Rodwell 11.389
Grimsley 11.242
Solomon 11.462
Lehninger 11.418
Nozaki 11.14
DTASelect 10.994
Thurlkill 11.155
EMBOSS 11.594
Sillero 11.169
Patrickios 11.14
IPC_peptide 11.477
IPC2_peptide 10.072
IPC2.peptide.svr19 8.551
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
172
0
172
49643
50
1213
288.6
33.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.691 ± 0.131
2.445 ± 0.123
6.45 ± 0.116
5.499 ± 0.167
4.494 ± 0.143
3.735 ± 0.17
2.445 ± 0.109
6.063 ± 0.112
6.774 ± 0.221
9.335 ± 0.144
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.605 ± 0.09
7.113 ± 0.139
3.706 ± 0.118
3.944 ± 0.126
4.839 ± 0.136
6.339 ± 0.167
6.142 ± 0.158
7.256 ± 0.128
0.971 ± 0.057
5.151 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here