Heterobasidion partitivirus 2
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1AB82|E1AB82_9VIRU Putative coat protein OS=Heterobasidion partitivirus 2 OX=872291 PE=4 SV=1
MM1 pKa = 7.05 STNPPEE7 pKa = 4.27 VLLPLPEE14 pKa = 4.2 VDD16 pKa = 3.79 PNANNVRR23 pKa = 11.84 YY24 pKa = 9.87 AKK26 pKa = 10.9 LLDD29 pKa = 3.52 QYY31 pKa = 10.96 RR32 pKa = 11.84 ANPSNSNQKK41 pKa = 10.44 LLLEE45 pKa = 4.36 YY46 pKa = 10.87 AEE48 pKa = 4.0 QHH50 pKa = 5.48 GFNYY54 pKa = 9.08 FIPTDD59 pKa = 3.52 VPLPDD64 pKa = 4.49 DD65 pKa = 4.2 RR66 pKa = 11.84 KK67 pKa = 9.51 SAPGILSLDD76 pKa = 3.52 GFRR79 pKa = 11.84 FHH81 pKa = 6.47 TVAAFHH87 pKa = 7.4 RR88 pKa = 11.84 YY89 pKa = 7.54 QKK91 pKa = 9.8 MSRR94 pKa = 11.84 YY95 pKa = 9.59 GYY97 pKa = 9.62 NALGFIFKK105 pKa = 10.89 LILTLFNPFLWILTDD120 pKa = 3.18 YY121 pKa = 10.55 CRR123 pKa = 11.84 PSGSADD129 pKa = 2.93 AVFEE133 pKa = 4.13 NFNQEE138 pKa = 4.02 VSPVEE143 pKa = 3.91 HH144 pKa = 6.44 VNPSRR149 pKa = 11.84 LAQIMPLIHH158 pKa = 6.79 HH159 pKa = 6.94 FFAIKK164 pKa = 9.36 PFCPIAFPDD173 pKa = 3.33 LRR175 pKa = 11.84 FYY177 pKa = 10.81 KK178 pKa = 10.08 WSLVTSADD186 pKa = 3.51 YY187 pKa = 10.17 HH188 pKa = 5.98 AHH190 pKa = 6.38 HH191 pKa = 7.1 SKK193 pKa = 10.68 DD194 pKa = 3.55 QQDD197 pKa = 3.47 EE198 pKa = 4.3 SAHH201 pKa = 4.8 YY202 pKa = 8.19 WKK204 pKa = 10.36 HH205 pKa = 6.37 LKK207 pKa = 10.72 DD208 pKa = 3.72 SDD210 pKa = 4.06 LLQDD214 pKa = 3.5 RR215 pKa = 11.84 FDD217 pKa = 3.83 YY218 pKa = 10.92 SDD220 pKa = 3.4 RR221 pKa = 11.84 PRR223 pKa = 11.84 SKK225 pKa = 10.57 GYY227 pKa = 10.26 FFNTVLLSTRR237 pKa = 11.84 TIVHH241 pKa = 6.64 NIKK244 pKa = 9.99 YY245 pKa = 9.83 HH246 pKa = 5.78 CLPFQRR252 pKa = 11.84 HH253 pKa = 5.17 KK254 pKa = 10.9 RR255 pKa = 11.84 DD256 pKa = 3.27 TDD258 pKa = 3.64 SSVLQKK264 pKa = 11.0 LSFWFMKK271 pKa = 10.1 YY272 pKa = 7.74 PTVMYY277 pKa = 10.34 VRR279 pKa = 11.84 SQISKK284 pKa = 9.79 LSKK287 pKa = 9.66 LKK289 pKa = 10.44 VRR291 pKa = 11.84 PVYY294 pKa = 9.92 NAPFLFILIEE304 pKa = 4.2 AMLTLALMAQCRR316 pKa = 11.84 LPDD319 pKa = 3.58 SCLMWGFEE327 pKa = 4.28 TVRR330 pKa = 11.84 GGMQEE335 pKa = 4.17 LNRR338 pKa = 11.84 ISYY341 pKa = 10.69 NYY343 pKa = 8.84 DD344 pKa = 3.17 TFIMIDD350 pKa = 3.26 WSRR353 pKa = 11.84 YY354 pKa = 8.48 DD355 pKa = 3.21 QLLPFAIIYY364 pKa = 7.86 HH365 pKa = 6.0 FWCTFLPQLIRR376 pKa = 11.84 VDD378 pKa = 4.09 LGYY381 pKa = 10.01 MPTEE385 pKa = 4.17 QYY387 pKa = 9.11 TATQHH392 pKa = 5.42 KK393 pKa = 9.52 HH394 pKa = 5.51 AFTEE398 pKa = 3.87 KK399 pKa = 10.83 HH400 pKa = 5.81 NDD402 pKa = 3.36 QKK404 pKa = 11.4 EE405 pKa = 4.49 SNPEE409 pKa = 3.69 YY410 pKa = 10.34 ATFASRR416 pKa = 11.84 LKK418 pKa = 8.86 THH420 pKa = 7.07 APHH423 pKa = 7.38 IVMFSFIIFNLLAFIWLWYY442 pKa = 9.44 VKK444 pKa = 9.62 MVFVTPDD451 pKa = 2.61 GFGYY455 pKa = 10.45 VRR457 pKa = 11.84 LLAGVPSGIFMTQICDD473 pKa = 3.68 SFCNAFLLIDD483 pKa = 3.81 AMLEE487 pKa = 4.12 FGFTPDD493 pKa = 4.12 DD494 pKa = 3.39 IKK496 pKa = 10.87 LIRR499 pKa = 11.84 MFIQGDD505 pKa = 3.96 DD506 pKa = 3.4 NVIFYY511 pKa = 10.84 LGDD514 pKa = 3.4 FTRR517 pKa = 11.84 IFAFYY522 pKa = 9.59 EE523 pKa = 3.88 WLPEE527 pKa = 3.8 YY528 pKa = 10.31 CQQRR532 pKa = 11.84 WHH534 pKa = 5.54 MTISVDD540 pKa = 3.08 KK541 pKa = 11.24 SSITRR546 pKa = 11.84 LRR548 pKa = 11.84 NKK550 pKa = 10.0 IEE552 pKa = 3.74 VLGYY556 pKa = 9.4 TNLNGMPHH564 pKa = 7.08 RR565 pKa = 11.84 DD566 pKa = 3.66 CAKK569 pKa = 10.73 LIATLAYY576 pKa = 9.14 PEE578 pKa = 5.64 RR579 pKa = 11.84 YY580 pKa = 9.46 VQDD583 pKa = 3.17 KK584 pKa = 9.37 TKK586 pKa = 10.91 YY587 pKa = 8.47 IVFMSRR593 pKa = 11.84 AIGIAYY599 pKa = 9.92 ANAGHH604 pKa = 6.99 DD605 pKa = 3.68 RR606 pKa = 11.84 QVHH609 pKa = 5.51 DD610 pKa = 4.3 LCQRR614 pKa = 11.84 AYY616 pKa = 10.42 LQARR620 pKa = 11.84 KK621 pKa = 10.04 DD622 pKa = 3.42 SGLSYY627 pKa = 11.39 DD628 pKa = 3.55 EE629 pKa = 4.82 LKK631 pKa = 10.86 NIKK634 pKa = 9.56 IEE636 pKa = 3.99 YY637 pKa = 8.7 QKK639 pKa = 11.25 LGFYY643 pKa = 10.4 EE644 pKa = 3.94 IFSVNIEE651 pKa = 4.11 EE652 pKa = 4.59 LRR654 pKa = 11.84 EE655 pKa = 3.85 HH656 pKa = 7.53 LIQDD660 pKa = 3.29 VSEE663 pKa = 4.6 FPNFHH668 pKa = 7.79 DD669 pKa = 3.4 IRR671 pKa = 11.84 DD672 pKa = 4.13 NLRR675 pKa = 11.84 HH676 pKa = 4.53 WHH678 pKa = 6.38 GPHH681 pKa = 5.59 TVYY684 pKa = 10.85 PMWPRR689 pKa = 11.84 HH690 pKa = 5.63 FDD692 pKa = 3.85 DD693 pKa = 5.4 DD694 pKa = 4.4 LSSIKK699 pKa = 10.47 SPHH702 pKa = 6.52 SLTTLYY708 pKa = 11.14 DD709 pKa = 3.56 VMQSGGLTFDD719 pKa = 3.9 YY720 pKa = 11.28 NFF722 pKa = 3.99
Molecular weight: 84.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.484
IPC2_protein 6.504
IPC_protein 6.605
Toseland 6.839
ProMoST 7.029
Dawson 7.044
Bjellqvist 7.015
Wikipedia 7.044
Rodwell 7.044
Grimsley 7.0
Solomon 7.044
Lehninger 7.044
Nozaki 7.322
DTASelect 7.439
Thurlkill 7.468
EMBOSS 7.454
Sillero 7.454
Patrickios 3.808
IPC_peptide 7.059
IPC2_peptide 7.117
IPC2.peptide.svr19 6.961
Protein with the highest isoelectric point:
>tr|E1AB82|E1AB82_9VIRU Putative coat protein OS=Heterobasidion partitivirus 2 OX=872291 PE=4 SV=1
MM1 pKa = 7.78 ADD3 pKa = 3.44 KK4 pKa = 10.76 VDD6 pKa = 3.74 PPRR9 pKa = 11.84 DD10 pKa = 3.7 VVAEE14 pKa = 4.35 TPPVPQPASSGRR26 pKa = 11.84 TVVKK30 pKa = 10.13 QRR32 pKa = 11.84 HH33 pKa = 5.33 PAQGAPTISLAKK45 pKa = 10.65 VLDD48 pKa = 4.11 LEE50 pKa = 4.6 SDD52 pKa = 3.68 VHH54 pKa = 7.12 KK55 pKa = 11.18 GKK57 pKa = 8.74 GTYY60 pKa = 9.81 HH61 pKa = 6.43 SGSYY65 pKa = 10.27 VPDD68 pKa = 2.96 VRR70 pKa = 11.84 FFVLSLCTYY79 pKa = 7.87 YY80 pKa = 11.16 GRR82 pKa = 11.84 VFSATDD88 pKa = 3.38 YY89 pKa = 9.55 EE90 pKa = 4.42 ASQFVTPPSLIAYY103 pKa = 9.48 SLFCLYY109 pKa = 10.64 FLLFYY114 pKa = 10.85 RR115 pKa = 11.84 DD116 pKa = 3.7 MHH118 pKa = 6.79 SKK120 pKa = 10.56 SPSQYY125 pKa = 10.72 ARR127 pKa = 11.84 TFTDD131 pKa = 4.18 FGRR134 pKa = 11.84 YY135 pKa = 7.94 NQLLKK140 pKa = 11.3 LMDD143 pKa = 3.74 EE144 pKa = 4.64 VYY146 pKa = 10.51 IPDD149 pKa = 4.68 EE150 pKa = 4.01 IFEE153 pKa = 4.37 LLKK156 pKa = 10.99 VFSVWSPEE164 pKa = 4.42 LIPNLQFVPSLGSTLPLYY182 pKa = 9.69 DD183 pKa = 3.97 IPYY186 pKa = 9.62 LMHH189 pKa = 6.92 PAIFLHH195 pKa = 5.87 GHH197 pKa = 4.6 NTLFNRR203 pKa = 11.84 ADD205 pKa = 3.52 AVGHH209 pKa = 4.98 YY210 pKa = 10.78 ARR212 pKa = 11.84 FLRR215 pKa = 11.84 TEE217 pKa = 4.16 LFSINSASLGNAARR231 pKa = 11.84 IMTIGNLIGCAYY243 pKa = 8.88 TPNATQGSARR253 pKa = 11.84 LIQNWLSNAILPFVDD268 pKa = 3.97 PATHH272 pKa = 6.05 RR273 pKa = 11.84 QHH275 pKa = 6.99 LRR277 pKa = 11.84 RR278 pKa = 11.84 TGLAKK283 pKa = 10.37 YY284 pKa = 10.4 DD285 pKa = 3.65 LGLPTFTSANWNPYY299 pKa = 8.67 TFLFSSNVSSSFEE312 pKa = 4.05 SFIDD316 pKa = 3.78 CAHH319 pKa = 6.65 ACSEE323 pKa = 4.34 FSRR326 pKa = 11.84 DD327 pKa = 3.25 QLKK330 pKa = 10.63 ASRR333 pKa = 11.84 HH334 pKa = 4.43 MSDD337 pKa = 4.83 LYY339 pKa = 11.12 SQVSPAPGSYY349 pKa = 9.65 MIQSYY354 pKa = 7.35 STPTWHH360 pKa = 6.59 ATSLPSASFPDD371 pKa = 4.22 GEE373 pKa = 4.3 ITRR376 pKa = 11.84 GDD378 pKa = 4.2 FDD380 pKa = 4.4 TLHH383 pKa = 5.13 TTYY386 pKa = 10.14 TFFGIAPAAATANSNNLHH404 pKa = 5.61 MPEE407 pKa = 4.07 VRR409 pKa = 11.84 QNPATATSTLIGFVPNAYY427 pKa = 10.29 LIEE430 pKa = 4.08 QHH432 pKa = 7.02 ADD434 pKa = 3.09 PDD436 pKa = 4.05 VAVPQQLSLIDD447 pKa = 3.9 RR448 pKa = 11.84 NEE450 pKa = 4.2 MKK452 pKa = 9.36 HH453 pKa = 5.01 TRR455 pKa = 11.84 PNILVFAPGDD465 pKa = 4.01 STVSAAQWPILTGIVVHH482 pKa = 6.53 NGNIDD487 pKa = 3.85 SNVIKK492 pKa = 10.59 APNPDD497 pKa = 3.91 DD498 pKa = 5.43 DD499 pKa = 4.9 LPQIRR504 pKa = 11.84 SRR506 pKa = 11.84 YY507 pKa = 8.11 VDD509 pKa = 3.25 GFVSLRR515 pKa = 11.84 VIRR518 pKa = 11.84 PRR520 pKa = 11.84 FANRR524 pKa = 11.84 PTWLIARR531 pKa = 11.84 QALEE535 pKa = 3.93 RR536 pKa = 11.84 VSYY539 pKa = 9.31 STINFLWRR547 pKa = 11.84 SDD549 pKa = 4.15 LIAVPRR555 pKa = 11.84 FDD557 pKa = 4.28 AAHH560 pKa = 6.42 NAPAAGGITGLLWPFTFFRR579 pKa = 11.84 RR580 pKa = 11.84 VTQMIFGTNVAVSDD594 pKa = 4.33 SPTDD598 pKa = 3.51 PTVASHH604 pKa = 6.12 KK605 pKa = 10.09 QVGDD609 pKa = 2.88 AWSSFRR615 pKa = 11.84 LTSDD619 pKa = 3.22 SNAVPSVEE627 pKa = 3.65 NTYY630 pKa = 10.86 FGINHH635 pKa = 6.5 GEE637 pKa = 3.93 IIFGRR642 pKa = 11.84 NSTLNKK648 pKa = 9.8 FSHH651 pKa = 6.78 PSDD654 pKa = 3.42 LLRR657 pKa = 11.84 RR658 pKa = 11.84 NN659 pKa = 3.45
Molecular weight: 73.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.492
IPC2_protein 6.402
IPC_protein 6.561
Toseland 6.912
ProMoST 6.971
Dawson 6.956
Bjellqvist 6.898
Wikipedia 6.971
Rodwell 6.942
Grimsley 7.088
Solomon 6.956
Lehninger 6.956
Nozaki 7.205
DTASelect 7.38
Thurlkill 7.41
EMBOSS 7.395
Sillero 7.337
Patrickios 4.062
IPC_peptide 6.971
IPC2_peptide 7.146
IPC2.peptide.svr19 6.977
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1381
659
722
690.5
79.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.024 ± 1.111
1.159 ± 0.273
6.155 ± 0.369
3.186 ± 0.415
6.951 ± 0.499
4.345 ± 0.453
3.838 ± 0.341
5.865 ± 0.379
3.476 ± 0.924
9.486 ± 0.468
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.245 ± 0.497
4.779 ± 0.26
6.517 ± 0.732
3.765 ± 0.292
5.576 ± 0.13
7.893 ± 1.243
5.793 ± 0.812
5.576 ± 0.545
1.593 ± 0.155
4.779 ± 0.674
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here