Vibrio phage vB_VcaS_HC

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T479|A0A4D6T479_9CAUD Uncharacterized protein OS=Vibrio phage vB_VcaS_HC OX=2493116 GN=vBVcaS_HC112 PE=4 SV=1
MM1 pKa = 7.58HH2 pKa = 7.46NLNNDD7 pKa = 3.64LFFVQPVNYY16 pKa = 9.82LNTEE20 pKa = 3.96GGVSYY25 pKa = 10.82EE26 pKa = 3.85AMTAEE31 pKa = 5.35FGDD34 pKa = 4.27GYY36 pKa = 11.56NRR38 pKa = 11.84INTTLFEE45 pKa = 4.93DD46 pKa = 3.72KK47 pKa = 11.01VGILIFRR54 pKa = 11.84NTDD57 pKa = 3.55LEE59 pKa = 4.07QGAALDD65 pKa = 3.97VEE67 pKa = 4.74KK68 pKa = 10.93GDD70 pKa = 4.38TIPNPKK76 pKa = 9.63PGEE79 pKa = 3.8NAIALNFHH87 pKa = 6.24TLQSIDD93 pKa = 3.48AVIGRR98 pKa = 11.84LQYY101 pKa = 10.07IRR103 pKa = 11.84EE104 pKa = 4.17VYY106 pKa = 10.53LEE108 pKa = 4.07RR109 pKa = 11.84EE110 pKa = 3.67EE111 pKa = 4.16AARR114 pKa = 11.84NKK116 pKa = 10.0PLEE119 pKa = 4.8AGDD122 pKa = 3.87KK123 pKa = 10.96CPTDD127 pKa = 3.55DD128 pKa = 5.27CEE130 pKa = 6.28GEE132 pKa = 4.23LEE134 pKa = 4.66HH135 pKa = 8.04YY136 pKa = 10.17PDD138 pKa = 3.7EE139 pKa = 4.69CRR141 pKa = 11.84CDD143 pKa = 3.9VVPPPCTACTDD154 pKa = 3.75APLSCCVCGWSEE166 pKa = 3.97EE167 pKa = 4.22DD168 pKa = 3.55GQGVII173 pKa = 5.21

Molecular weight: 19.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T482|A0A4D6T482_9CAUD Uncharacterized protein OS=Vibrio phage vB_VcaS_HC OX=2493116 GN=vBVcaS_HC088 PE=4 SV=1
MM1 pKa = 7.72CNTRR5 pKa = 11.84RR6 pKa = 11.84YY7 pKa = 10.43SPTVSDD13 pKa = 3.2KK14 pKa = 11.32RR15 pKa = 11.84GFNGEE20 pKa = 3.98AMEE23 pKa = 4.12QRR25 pKa = 11.84PNGNYY30 pKa = 9.7VLWTTHH36 pKa = 6.04HH37 pKa = 6.3NMINHH42 pKa = 6.03LQRR45 pKa = 11.84RR46 pKa = 11.84IRR48 pKa = 11.84QLEE51 pKa = 4.11KK52 pKa = 10.67KK53 pKa = 10.54LEE55 pKa = 4.17NATKK59 pKa = 10.36NQQ61 pKa = 3.27

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

113

0

113

24095

47

1377

213.2

23.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.94 ± 0.374

1.22 ± 0.139

6.014 ± 0.174

7.4 ± 0.233

3.615 ± 0.149

6.055 ± 0.203

2.042 ± 0.164

5.935 ± 0.159

6.117 ± 0.469

9.189 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.12

5.196 ± 0.162

3.968 ± 0.195

4.495 ± 0.256

5.657 ± 0.24

5.491 ± 0.205

6.308 ± 0.242

6.217 ± 0.224

0.784 ± 0.08

3.192 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski