Streptomyces phage VWB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6VY85|Q6VY85_9CAUD Uncharacterized protein OS=Streptomyces phage VWB OX=10702 PE=4 SV=1
MM1 pKa = 7.46SAIDD5 pKa = 3.73KK6 pKa = 10.32ARR8 pKa = 11.84KK9 pKa = 7.09QARR12 pKa = 11.84EE13 pKa = 3.99LLDD16 pKa = 4.32LVTGTPTPEE25 pKa = 3.3WRR27 pKa = 11.84VCFSEE32 pKa = 5.66DD33 pKa = 3.11GTEE36 pKa = 4.09EE37 pKa = 4.02PTGIAPVCLDD47 pKa = 4.22EE48 pKa = 5.88GHH50 pKa = 7.17DD51 pKa = 3.97PDD53 pKa = 6.12DD54 pKa = 4.7GSVYY58 pKa = 10.46DD59 pKa = 4.37CCPSPVVEE67 pKa = 4.87CDD69 pKa = 3.2SDD71 pKa = 3.83PLAAYY76 pKa = 9.81LVEE79 pKa = 4.88LLNADD84 pKa = 4.04RR85 pKa = 11.84GQAA88 pKa = 3.21

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6VY62|Q6VY62_9CAUD Uncharacterized protein OS=Streptomyces phage VWB OX=10702 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84RR3 pKa = 11.84GLVVRR8 pKa = 11.84VPIDD12 pKa = 3.44PPQRR16 pKa = 11.84IACVARR22 pKa = 11.84SRR24 pKa = 11.84HH25 pKa = 4.29GRR27 pKa = 11.84PRR29 pKa = 11.84AVAPVVPAEE38 pKa = 4.68EE39 pKa = 4.88PPDD42 pKa = 3.77DD43 pKa = 5.14CGIIDD48 pKa = 3.98TNLPTVKK55 pKa = 10.4VLWEE59 pKa = 3.54RR60 pKa = 11.84RR61 pKa = 11.84GRR63 pKa = 11.84VMRR66 pKa = 11.84LMTVTEE72 pKa = 4.28PAPPYY77 pKa = 9.62LPPWAPYY84 pKa = 9.42LPPWKK89 pKa = 10.04RR90 pKa = 11.84SAPEE94 pKa = 3.41

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

13642

51

1440

223.6

24.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.634 ± 0.468

1.041 ± 0.176

6.473 ± 0.403

5.578 ± 0.439

2.067 ± 0.184

8.716 ± 0.425

2.111 ± 0.265

3.541 ± 0.258

2.404 ± 0.365

8.437 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.818 ± 0.114

1.561 ± 0.181

7.404 ± 0.632

2.976 ± 0.23

9.236 ± 0.682

5.08 ± 0.29

6.319 ± 0.312

7.572 ± 0.335

2.148 ± 0.137

1.884 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski