Rhodoluna lacicola
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1364 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060JEM4|A0A060JEM4_9MICO o-succinylbenzoate synthase OS=Rhodoluna lacicola OX=529884 GN=menC PE=3 SV=1
MM1 pKa = 7.39 PKK3 pKa = 7.96 WWKK6 pKa = 9.97 IGFVSVLLVTTGALSAYY23 pKa = 9.83 VALQEE28 pKa = 4.07 PTLDD32 pKa = 3.77 EE33 pKa = 4.91 EE34 pKa = 4.53 ISAQVAEE41 pKa = 4.57 YY42 pKa = 11.17 ADD44 pKa = 3.61 TDD46 pKa = 3.77 YY47 pKa = 11.45 LYY49 pKa = 10.41 WDD51 pKa = 3.58 WADD54 pKa = 3.59 EE55 pKa = 4.33 AVCDD59 pKa = 5.18 LYY61 pKa = 11.21 EE62 pKa = 4.18 RR63 pKa = 11.84 CQFIEE68 pKa = 3.99 IYY70 pKa = 9.36 DD71 pKa = 3.88 TARR74 pKa = 11.84 CADD77 pKa = 3.83 QILVPMRR84 pKa = 11.84 LEE86 pKa = 4.35 DD87 pKa = 4.29 VNEE90 pKa = 4.14 DD91 pKa = 3.04 WVAAASTVMRR101 pKa = 11.84 SPGKK105 pKa = 9.46 HH106 pKa = 5.26 GGVLVEE112 pKa = 4.52 LGVNRR117 pKa = 11.84 ADD119 pKa = 3.62 FEE121 pKa = 4.52 YY122 pKa = 10.53 FAIGDD127 pKa = 3.79 VRR129 pKa = 11.84 CTYY132 pKa = 11.15 ALPTGEE138 pKa = 4.9 AGLL141 pKa = 4.09
Molecular weight: 15.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.838
IPC2_protein 4.037
IPC_protein 3.986
Toseland 3.795
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.859
Rodwell 3.821
Grimsley 3.706
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.253
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.101
Patrickios 0.744
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 3.992
Protein with the highest isoelectric point:
>tr|A0A060JKP6|A0A060JKP6_9MICO Putative phosphohydrolase OS=Rhodoluna lacicola OX=529884 GN=Rhola_00003160 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.79 HH17 pKa = 5.67 RR18 pKa = 11.84 KK19 pKa = 8.31 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1364
0
1364
450371
31
7963
330.2
35.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.446 ± 0.083
0.515 ± 0.014
5.338 ± 0.06
6.015 ± 0.105
3.759 ± 0.041
8.265 ± 0.093
1.752 ± 0.038
6.121 ± 0.052
4.85 ± 0.071
9.79 ± 0.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.035 ± 0.03
3.512 ± 0.057
4.332 ± 0.043
3.267 ± 0.036
5.188 ± 0.077
6.49 ± 0.1
5.754 ± 0.151
8.067 ± 0.06
1.266 ± 0.03
2.239 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here