Proteome-pI 2.0 - Proteome Isoelectric Point Database

Chryseobacterium sp. MOF25P

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Chryseobacterium group; Chryseobacterium; unclassified Chryseobacterium

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 4239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1A7KLE7|A0A1A7KLE7_9FLAO Ferric uptake regulation protein OS=Chryseobacterium sp. MOF25P OX=1664318 GN=fur PE=3 SV=1

MM1 pKa = 6.77NTQVLDD7 pKa = 4.55FWAGNFRR14 pKa = 11.84TEE16 pKa = 3.41KK17 pKa = 10.93DD18 pKa = 3.05FFQFVEE24 pKa = 4.11EE25 pKa = 4.42DD26 pKa = 3.04EE27 pKa = 4.41NYY29 pKa = 10.77YY30 pKa = 10.01IEE32 pKa = 4.93EE33 pKa = 4.43EE34 pKa = 4.15SDD36 pKa = 3.04DD37 pKa = 3.94TYY39 pKa = 11.14ISKK42 pKa = 10.16FAEE45 pKa = 4.21SQDD48 pKa = 4.21TIWFDD53 pKa = 3.46QDD55 pKa = 2.85LMEE58 pKa = 4.86YY59 pKa = 10.58GFEE62 pKa = 3.99QSIQHH67 pKa = 5.59FSEE70 pKa = 4.72YY71 pKa = 11.01SFADD75 pKa = 2.77QWLPVLYY82 pKa = 10.7NRR84 pKa = 11.84LNEE87 pKa = 4.11MNLKK91 pKa = 10.17FDD93 pKa = 3.64INSLVFVSQGQIPTPKK109 pKa = 10.27SIEE112 pKa = 3.89NDD114 pKa = 3.79DD115 pKa = 4.13FSLTYY120 pKa = 10.39VGGIEE125 pKa = 4.49FEE127 pKa = 4.53YY128 pKa = 11.22

Molecular weight: 15.35 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1A7KPC4|A0A1A7KPC4_9FLAO Uncharacterized protein OS=Chryseobacterium sp. MOF25P OX=1664318 GN=AB670_00099 PE=4 SV=1

MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPSEE10 pKa = 3.58RR11 pKa = 11.84KK12 pKa = 9.37KK13 pKa = 10.2RR14 pKa = 11.84NKK16 pKa = 9.71HH17 pKa = 4.09GFRR20 pKa = 11.84EE21 pKa = 4.33RR22 pKa = 11.84MSTPNGRR29 pKa = 11.84RR30 pKa = 11.84VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.99GRR40 pKa = 11.84KK41 pKa = 8.47SLSVSSARR49 pKa = 11.84AKK51 pKa = 10.42RR52 pKa = 3.45

Molecular weight: 6.06 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
4239	0	4239	1340762	29	2359	316.3	35.86

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.923 ± 0.044	0.735 ± 0.013	5.379 ± 0.029	6.67 ± 0.048	5.541 ± 0.037	6.09 ± 0.036	1.612 ± 0.016	8.109 ± 0.038	8.566 ± 0.052	8.99 ± 0.035

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.267 ± 0.02	6.729 ± 0.039	3.266 ± 0.022	3.55 ± 0.02	3.111 ± 0.025	6.737 ± 0.037	5.643 ± 0.046	5.872 ± 0.03	1.038 ± 0.011	4.172 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski