Polynucleobacter sp. VK13
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2757 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W1ZC72|A0A1W1ZC72_9BURK Putative phosphoenolpyruvate synthase regulatory protein OS=Polynucleobacter sp. VK13 OX=1938817 GN=SAMN06296008_10516 PE=3 SV=1
MM1 pKa = 7.57 TSTIAPVLPINSTADD16 pKa = 3.47 LFIPDD21 pKa = 4.35 LATAVSGANPLSSRR35 pKa = 11.84 YY36 pKa = 10.31 VIDD39 pKa = 3.89 ARR41 pKa = 11.84 TGGNYY46 pKa = 9.72 IIRR49 pKa = 11.84 AVQVTMDD56 pKa = 3.4 VGLFGAANTIMWAYY70 pKa = 10.19 EE71 pKa = 4.39 LIDD74 pKa = 3.99 PTTGSAMTSLPNIPGLIMGDD94 pKa = 3.8 PVNSALTGSAALSAGAPILNPTFLVQGNSIVGDD127 pKa = 3.79 GTNVPISITWQNGLPIGGHH146 pKa = 6.66 LLPLDD151 pKa = 3.81 MSVPMAGMDD160 pKa = 3.82 SMGSMSGMPGMNASTNTMCTVTTPTGVYY188 pKa = 7.54 MVPMTTHH195 pKa = 4.18 VHH197 pKa = 5.75 GAHH200 pKa = 5.89 VAAIYY205 pKa = 10.27 DD206 pKa = 3.94 GNPNLTITPDD216 pKa = 3.34 QVVTYY221 pKa = 10.69 VYY223 pKa = 11.29 DD224 pKa = 3.71 NTQKK228 pKa = 11.12 GAFIWYY234 pKa = 8.53 HH235 pKa = 4.9 DD236 pKa = 3.62 HH237 pKa = 7.45 SMGVTRR243 pKa = 11.84 LNVYY247 pKa = 10.42 AGLAGGYY254 pKa = 10.22 LIDD257 pKa = 4.0 SNTRR261 pKa = 11.84 EE262 pKa = 4.23 LVGEE266 pKa = 4.53 GPAAKK271 pKa = 9.89 AVNGLHH277 pKa = 6.9 LLPDD281 pKa = 3.51 ISKK284 pKa = 8.63 EE285 pKa = 4.19 VPVVVCDD292 pKa = 3.74 KK293 pKa = 11.14 SFRR296 pKa = 11.84 TDD298 pKa = 2.66 GSLFYY303 pKa = 9.98 PSQLADD309 pKa = 4.06 PLPGTGEE316 pKa = 3.75 IVADD320 pKa = 4.08 VQHH323 pKa = 7.4 DD324 pKa = 3.77 IYY326 pKa = 11.61 DD327 pKa = 3.35 MHH329 pKa = 6.43 GQLVYY334 pKa = 10.2 STANPFMLDD343 pKa = 3.3 TADD346 pKa = 5.1 SIAKK350 pKa = 9.76 LAALKK355 pKa = 10.18 DD356 pKa = 3.78 AYY358 pKa = 9.94 IINPDD363 pKa = 3.08 IKK365 pKa = 10.99 VVTLHH370 pKa = 6.88 AGEE373 pKa = 5.12 CFVCDD378 pKa = 3.49 MNGNLVKK385 pKa = 10.63 DD386 pKa = 3.92 DD387 pKa = 4.34 GKK389 pKa = 11.31 LQFFYY394 pKa = 11.12 DD395 pKa = 3.49 ATQPVGMRR403 pKa = 11.84 YY404 pKa = 9.24 ILSTPEE410 pKa = 4.04 SIAMHH415 pKa = 6.51 SATADD420 pKa = 3.41 TTKK423 pKa = 10.8 LYY425 pKa = 10.96 AFDD428 pKa = 5.1 CEE430 pKa = 4.11 TGRR433 pKa = 11.84 IYY435 pKa = 10.84 NSPTIPTNLYY445 pKa = 9.64 LQDD448 pKa = 4.49 SNGQDD453 pKa = 2.85 IVTKK457 pKa = 10.3 HH458 pKa = 5.07 QDD460 pKa = 2.82 IVIGGCYY467 pKa = 8.94 VHH469 pKa = 7.6 DD470 pKa = 4.5 EE471 pKa = 4.25 NNLLVSGNAQLQYY484 pKa = 10.75 FQDD487 pKa = 3.64 NQHH490 pKa = 6.57 PDD492 pKa = 2.98 KK493 pKa = 10.97 FILLTAEE500 pKa = 4.27 SGIKK504 pKa = 9.73 HH505 pKa = 6.18 HH506 pKa = 7.31 VDD508 pKa = 2.88 GTTPLYY514 pKa = 11.03 ALDD517 pKa = 4.51 CPTGKK522 pKa = 10.54 LYY524 pKa = 10.73 AGPTASGPYY533 pKa = 8.69 LQLVAGTDD541 pKa = 3.21 VTVPHH546 pKa = 6.92 FEE548 pKa = 4.35 LQPGCYY554 pKa = 9.59 VHH556 pKa = 7.7 DD557 pKa = 4.14 INNIPVIDD565 pKa = 3.99 NGSLEE570 pKa = 4.11 FFQLANGEE578 pKa = 4.83 FILSTPDD585 pKa = 3.81 SITTYY590 pKa = 8.31 PTEE593 pKa = 3.98 SATVYY598 pKa = 11.01 AFDD601 pKa = 5.14 CMTAKK606 pKa = 10.86 LFTTGHH612 pKa = 5.1 TTDD615 pKa = 3.25 NTFVIDD621 pKa = 3.78 PSTNVEE627 pKa = 4.05 AMVMHH632 pKa = 6.81 HH633 pKa = 6.36 EE634 pKa = 4.35 AQMSDD639 pKa = 4.08 CYY641 pKa = 11.26 VLDD644 pKa = 3.54 MNGNYY649 pKa = 10.01 VKK651 pKa = 9.94 DD652 pKa = 3.99 TPAGLLYY659 pKa = 10.7 FFDD662 pKa = 4.39 EE663 pKa = 4.66 ACGQYY668 pKa = 10.04 TLVTPEE674 pKa = 4.62 SIAMHH679 pKa = 6.95 NIMEE683 pKa = 4.46 TTPLYY688 pKa = 11.14 LLDD691 pKa = 4.08 CTNGKK696 pKa = 9.61 LFDD699 pKa = 3.66 STVNSGHH706 pKa = 5.82 YY707 pKa = 9.8 LQANGADD714 pKa = 4.07 VTVMMYY720 pKa = 8.19 DD721 pKa = 3.3 TNYY724 pKa = 10.56 VLDD727 pKa = 3.85 NFGNTVLDD735 pKa = 3.42 HH736 pKa = 7.08 AGAFFIEE743 pKa = 4.67 DD744 pKa = 3.53 TANPGTYY751 pKa = 10.52 VLDD754 pKa = 3.91 NTPHH758 pKa = 6.39 FSLNGGIYY766 pKa = 8.89 TLSEE770 pKa = 4.41 AGTLIQEE777 pKa = 3.86 TRR779 pKa = 11.84 YY780 pKa = 10.38 IKK782 pKa = 9.61 DD783 pKa = 3.29 TNIPQHH789 pKa = 5.95 YY790 pKa = 9.97 SFDD793 pKa = 4.19 NAHH796 pKa = 6.17 LTYY799 pKa = 10.31 VANVDD804 pKa = 3.24 GSYY807 pKa = 10.98 VADD810 pKa = 3.48 TANPGKK816 pKa = 10.66 YY817 pKa = 9.61 VVDD820 pKa = 4.14 TNPHH824 pKa = 5.24 YY825 pKa = 11.33 SIDD828 pKa = 3.6 STGAYY833 pKa = 10.29 VIDD836 pKa = 3.91 NANGTFVQDD845 pKa = 3.2 PTVYY849 pKa = 10.06 YY850 pKa = 11.0 VEE852 pKa = 4.43 DD853 pKa = 3.47 TTPRR857 pKa = 11.84 FTLDD861 pKa = 3.11 AVTHH865 pKa = 5.85 AVIDD869 pKa = 3.95 STGTPVLDD877 pKa = 5.7 AITGAPVLVDD887 pKa = 3.13 HH888 pKa = 6.91 FLPHH892 pKa = 6.58 FMKK895 pKa = 10.83 LPIPTAVPEE904 pKa = 4.14 YY905 pKa = 9.64 FGNVIMGNGQAWPNMTVAPGDD926 pKa = 3.75 VLIDD930 pKa = 3.96 LLNGSDD936 pKa = 3.48 SRR938 pKa = 11.84 FYY940 pKa = 11.47 DD941 pKa = 4.93 LMLDD945 pKa = 3.78 DD946 pKa = 5.59 PGVKK950 pKa = 8.62 VTLVGTDD957 pKa = 3.12 QGLLEE962 pKa = 4.29 HH963 pKa = 7.11 PIVLFNGDD971 pKa = 3.24 GVQEE975 pKa = 4.23 KK976 pKa = 10.81 GEE978 pKa = 4.14 HH979 pKa = 6.61 FIFAPADD986 pKa = 3.99 RR987 pKa = 11.84 YY988 pKa = 10.61 QLLFDD993 pKa = 4.53 FTNASQKK1000 pKa = 10.23 IIHH1003 pKa = 6.61 LEE1005 pKa = 4.04 NVGTAYY1011 pKa = 10.31 EE1012 pKa = 4.1 PFKK1015 pKa = 11.11 GLNADD1020 pKa = 3.88 GGLAGPVQHH1029 pKa = 7.27 IDD1031 pKa = 3.42 LSQDD1035 pKa = 2.98 PVGQVMSFTVDD1046 pKa = 3.0 QNQAATAFHH1055 pKa = 6.45 SAISAKK1061 pKa = 10.08 VDD1063 pKa = 4.25 AINAADD1069 pKa = 4.03 KK1070 pKa = 11.04 LAGTHH1075 pKa = 5.82 SLSAEE1080 pKa = 4.03 APAGEE1085 pKa = 4.58 SGMGGHH1091 pKa = 6.6 VLTDD1095 pKa = 3.57 ANLVLDD1101 pKa = 4.96 NSFQTYY1107 pKa = 10.5 KK1108 pKa = 10.87 LVPTSTLPTGSTVDD1122 pKa = 3.29 TVTYY1126 pKa = 10.95 GDD1128 pKa = 3.86 GTAVTATDD1136 pKa = 4.25 PNTILVDD1143 pKa = 3.83 AAAVTTRR1150 pKa = 11.84 KK1151 pKa = 9.85 IGVFEE1156 pKa = 4.3 VADD1159 pKa = 3.47 QYY1161 pKa = 11.59 GRR1163 pKa = 11.84 IMPQVGIAEE1172 pKa = 4.35 VKK1174 pKa = 10.21 VDD1176 pKa = 3.65 QTGKK1180 pKa = 8.78 TIQAGGLGYY1189 pKa = 10.03 DD1190 pKa = 3.36 QPTTEE1195 pKa = 5.32 IIQLKK1200 pKa = 10.32 DD1201 pKa = 3.03 GSKK1204 pKa = 9.9 PVTEE1208 pKa = 3.14 IWEE1211 pKa = 4.8 FYY1213 pKa = 10.98 NVTADD1218 pKa = 3.38 AHH1220 pKa = 6.21 PMHH1223 pKa = 6.79 IHH1225 pKa = 5.03 QVNFQVLGRR1234 pKa = 11.84 YY1235 pKa = 9.14 LLTEE1239 pKa = 4.36 TPTSGPNTGDD1249 pKa = 3.37 TNGDD1253 pKa = 3.88 GIVLTSEE1260 pKa = 3.89 ASYY1263 pKa = 11.75 NNDD1266 pKa = 2.94 FGTEE1270 pKa = 3.67 IPLYY1274 pKa = 9.77 ATDD1277 pKa = 5.44 AGAQDD1282 pKa = 4.38 TVWVGPGQGIKK1293 pKa = 10.6 VVMTFDD1299 pKa = 4.31 RR1300 pKa = 11.84 PGDD1303 pKa = 4.07 YY1304 pKa = 9.19 MWHH1307 pKa = 5.73 CHH1309 pKa = 5.5 ILSHH1313 pKa = 6.88 EE1314 pKa = 4.23 DD1315 pKa = 2.92 HH1316 pKa = 7.6 DD1317 pKa = 4.07 MMRR1320 pKa = 11.84 TLKK1323 pKa = 10.64 VVGVTGDD1330 pKa = 3.44 FEE1332 pKa = 5.79 GMVTEE1337 pKa = 5.38 DD1338 pKa = 3.42 SANAATGLIEE1348 pKa = 4.76 IGKK1351 pKa = 9.39 VDD1353 pKa = 3.45 QAMQGFTTDD1362 pKa = 3.13 IDD1364 pKa = 4.2 PVTKK1368 pKa = 10.59 ADD1370 pKa = 4.09 LGYY1373 pKa = 10.65 KK1374 pKa = 9.75 IYY1376 pKa = 10.51 QSQFGSLTMYY1386 pKa = 11.05 KK1387 pKa = 10.3 DD1388 pKa = 4.25 GNWSYY1393 pKa = 11.27 HH1394 pKa = 6.02 ADD1396 pKa = 3.5 NTNAAVQAIGLADD1409 pKa = 4.03 KK1410 pKa = 9.97 LTDD1413 pKa = 3.89 TITITEE1419 pKa = 4.36 LDD1421 pKa = 3.81 GTTHH1425 pKa = 6.86 NISVTVNGVNDD1436 pKa = 4.23 GPVITSNGGLATATIQIDD1454 pKa = 3.78 QYY1456 pKa = 11.7 APALITTLHH1465 pKa = 5.87 ATDD1468 pKa = 3.93 VDD1470 pKa = 4.3 TNDD1473 pKa = 3.37 VGLLQYY1479 pKa = 10.7 SIVGGADD1486 pKa = 2.91 QALFSIDD1493 pKa = 3.47 QATGALTLNNPTNFVAPTDD1512 pKa = 4.03 TNSDD1516 pKa = 2.88 LSYY1519 pKa = 10.46 EE1520 pKa = 4.34 VQVQVADD1527 pKa = 3.74 GHH1529 pKa = 5.97 GGKK1532 pKa = 9.43 AAQMIAVNAVWSGTTIVLTEE1552 pKa = 4.7 GIDD1555 pKa = 3.59 TYY1557 pKa = 11.51 VATGSADD1564 pKa = 3.34 FWIKK1568 pKa = 10.94 GLGSDD1573 pKa = 5.07 DD1574 pKa = 4.06 VLTGSTGNDD1583 pKa = 3.65 LIEE1586 pKa = 5.06 GGTGNDD1592 pKa = 4.42 LITGRR1597 pKa = 11.84 VGNDD1601 pKa = 2.99 NLIGGDD1607 pKa = 4.08 GIDD1610 pKa = 3.58 TASYY1614 pKa = 9.35 AAASGGVTVNLSTGLATGADD1634 pKa = 3.91 GSDD1637 pKa = 3.21 TLSTIEE1643 pKa = 4.62 NIIGSAFADD1652 pKa = 4.16 SLTGDD1657 pKa = 3.38 AGNNVLTGGAGLDD1670 pKa = 3.66 TFVVTGGTDD1679 pKa = 3.62 TITDD1683 pKa = 4.27 LGKK1686 pKa = 10.92 GGADD1690 pKa = 2.88 ILTVAVGATANATVTSAWTATATSVNSGTEE1720 pKa = 4.15 NITSSGLAVNLVAITTGNGFTVTNTGIAASFTGSSLNDD1758 pKa = 3.52 TLIGGTGNDD1767 pKa = 4.03 TLNGGAGNDD1776 pKa = 3.89 LLRR1779 pKa = 11.84 GGLGKK1784 pKa = 10.27 DD1785 pKa = 3.28 VLIGGTGIDD1794 pKa = 3.5 TVSYY1798 pKa = 10.2 SAATAAVNVNLTTGLATGADD1818 pKa = 3.91 GSDD1821 pKa = 3.21 TLSTIEE1827 pKa = 4.62 NIIGSAFADD1836 pKa = 4.16 SLTGDD1841 pKa = 3.38 AGNNVLTGDD1850 pKa = 3.79 AGLDD1854 pKa = 3.56 TFVVTGGTDD1863 pKa = 3.62 TITDD1867 pKa = 4.56 LGRR1870 pKa = 11.84 GGADD1874 pKa = 2.72 ILTVAAGATANATVTSAWTASSASVNKK1901 pKa = 8.7 GTEE1904 pKa = 4.34 NITSSGFAVNLTAVTTGNGFTVTNTGVAAALTGSGLNDD1942 pKa = 3.49 NLIGGAGNDD1951 pKa = 3.89 TLIGGAGNDD1960 pKa = 3.75 TLTGVAGNDD1969 pKa = 2.97 ILTGGTGIDD1978 pKa = 3.67 TFTVSAGVDD1987 pKa = 3.67 TITDD1991 pKa = 4.46 LGNGGADD1998 pKa = 3.01 ILTVATGATANATITSAWTATATSVNNGAEE2028 pKa = 4.32 NIASAGLAVNLAAVTTGNGFTVTNTGVATSFTGSSLNDD2066 pKa = 3.59 TLNGGVGNDD2075 pKa = 3.88 TLSGGAGNDD2084 pKa = 3.76 LLNGGLGNDD2093 pKa = 3.94 ALTGGTGIDD2102 pKa = 3.67 TFTVSAGVDD2111 pKa = 3.7 TINDD2115 pKa = 3.98 LGIGGADD2122 pKa = 3.27 ILIVSAGATANAKK2135 pKa = 8.19 VTATYY2140 pKa = 10.28 AATVATSNAGIANISTGGFNVDD2162 pKa = 3.22 LTLATGTVGYY2172 pKa = 10.72 SLTNTGNAAVLTGSGLNDD2190 pKa = 3.36 TLMGGTGNDD2199 pKa = 3.74 TLNGGAGDD2207 pKa = 3.9 DD2208 pKa = 4.17 LLRR2211 pKa = 11.84 GGLGNDD2217 pKa = 3.69 VIIGGTGVDD2226 pKa = 3.52 TVSYY2230 pKa = 10.17 SAATGSVNVNLTTGLATGADD2250 pKa = 3.67 GSDD2253 pKa = 3.22 TFRR2256 pKa = 11.84 TIEE2259 pKa = 4.3 NIIGSAFADD2268 pKa = 4.11 TLTGDD2273 pKa = 3.42 AGNNVLTGGAGVDD2286 pKa = 3.66 TFTVSAGIDD2295 pKa = 3.64 TITDD2299 pKa = 4.31 LGNGGADD2306 pKa = 3.18 ILTVAASATANATVTSAWTATATSVNKK2333 pKa = 10.56 GIEE2336 pKa = 4.39 NISTAGLAVNLAAVTTGNGFTVTNTGVAAALTGSGLNDD2374 pKa = 3.53 TLIGGVGNDD2383 pKa = 3.68 TLSGGVGNDD2392 pKa = 2.78 ILTGGNGSDD2401 pKa = 3.3 VFVFKK2406 pKa = 11.02 NAFGTNNIDD2415 pKa = 3.6 TITDD2419 pKa = 4.34 FLSKK2423 pKa = 9.85 TDD2425 pKa = 3.57 TVQLSKK2431 pKa = 11.1 AIFSALGNVNQSITANQWLEE2451 pKa = 4.49 TTNHH2455 pKa = 6.19 TATSQTQRR2463 pKa = 11.84 VIHH2466 pKa = 6.2 DD2467 pKa = 3.84 TVDD2470 pKa = 2.72 GGLYY2474 pKa = 10.22 YY2475 pKa = 10.82 DD2476 pKa = 5.01 ADD2478 pKa = 3.92 GCGAIAAVKK2487 pKa = 9.82 IAVIGVDD2494 pKa = 3.34 HH2495 pKa = 6.15 MVIGDD2500 pKa = 3.66 ISVIAA2505 pKa = 4.31
Molecular weight: 257.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.872
IPC_protein 3.948
Toseland 3.706
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.91
Rodwell 3.77
Grimsley 3.605
Solomon 3.948
Lehninger 3.91
Nozaki 4.05
DTASelect 4.368
Thurlkill 3.757
EMBOSS 3.923
Sillero 4.075
Patrickios 0.947
IPC_peptide 3.948
IPC2_peptide 4.05
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|A0A1W2BVM4|A0A1W2BVM4_9BURK Rhodanese-related sulfurtransferase OS=Polynucleobacter sp. VK13 OX=1938817 GN=SAMN06296008_11614 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.43 RR14 pKa = 11.84 THH16 pKa = 5.88 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.67 TKK25 pKa = 10.49 GGRR28 pKa = 11.84 KK29 pKa = 8.69 VLNNRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.61 GRR39 pKa = 11.84 KK40 pKa = 8.69 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2757
0
2757
888343
29
5292
322.2
35.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.529 ± 0.051
0.916 ± 0.016
4.918 ± 0.039
5.631 ± 0.059
4.281 ± 0.038
7.266 ± 0.063
2.188 ± 0.027
7.116 ± 0.039
5.736 ± 0.057
10.24 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.628 ± 0.028
4.508 ± 0.057
4.606 ± 0.033
4.279 ± 0.036
4.236 ± 0.045
6.647 ± 0.05
5.452 ± 0.081
6.734 ± 0.033
1.269 ± 0.018
2.82 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here