Facklamia miroungae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Facklamia

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1889 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7NY20|A0A1G7NY20_9LACT Uncharacterized protein OS=Facklamia miroungae OX=120956 GN=SAMN05421791_10122 PE=4 SV=1
MM1 pKa = 7.38FSIKK5 pKa = 10.17YY6 pKa = 10.15VSLLAPATAGNQDD19 pKa = 3.08QVLPMLVVKK28 pKa = 10.45YY29 pKa = 9.64EE30 pKa = 4.03FEE32 pKa = 4.08NTSDD36 pKa = 3.73QKK38 pKa = 11.73VMDD41 pKa = 4.44YY42 pKa = 11.37AHH44 pKa = 7.23AWDD47 pKa = 3.59QQVFFSQFKK56 pKa = 8.82EE57 pKa = 3.78DD58 pKa = 3.81SMNKK62 pKa = 9.95LEE64 pKa = 4.27PANYY68 pKa = 9.81QLDD71 pKa = 3.88PDD73 pKa = 3.79QEE75 pKa = 4.32VFPKK79 pKa = 10.72YY80 pKa = 10.51EE81 pKa = 4.12EE82 pKa = 4.22VDD84 pKa = 3.38SGEE87 pKa = 4.19QTTVTAYY94 pKa = 9.68YY95 pKa = 10.9QLMDD99 pKa = 3.91TEE101 pKa = 5.05SPLTLSIAEE110 pKa = 4.55NEE112 pKa = 4.47TISDD116 pKa = 3.95FDD118 pKa = 4.96LKK120 pKa = 10.85IEE122 pKa = 5.14DD123 pKa = 4.57LLKK126 pKa = 10.94LPNPSALYY134 pKa = 10.95LNDD137 pKa = 3.77SNQGYY142 pKa = 9.2LFDD145 pKa = 5.75FNTLYY150 pKa = 11.03VLNPSQDD157 pKa = 3.38LVNQLDD163 pKa = 4.48LEE165 pKa = 4.36IQNPSDD171 pKa = 3.64FEE173 pKa = 4.24LSKK176 pKa = 10.6EE177 pKa = 4.23ANVQLEE183 pKa = 4.31KK184 pKa = 11.14LNEE187 pKa = 3.99NDD189 pKa = 3.0VEE191 pKa = 4.56AIKK194 pKa = 10.93LEE196 pKa = 4.38NINYY200 pKa = 9.25QVTEE204 pKa = 4.09EE205 pKa = 4.05EE206 pKa = 4.1QIEE209 pKa = 4.66VINEE213 pKa = 3.91YY214 pKa = 11.06EE215 pKa = 4.17EE216 pKa = 5.05VILTLEE222 pKa = 4.44SQSNWNDD229 pKa = 3.24FEE231 pKa = 4.85DD232 pKa = 5.48LNGQMYY238 pKa = 10.09QIVEE242 pKa = 4.04

Molecular weight: 28.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7TT11|A0A1G7TT11_9LACT Glycerol kinase OS=Facklamia miroungae OX=120956 GN=glpK PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.15QKK5 pKa = 7.56THH7 pKa = 6.75RR8 pKa = 11.84GLAKK12 pKa = 9.89RR13 pKa = 11.84VKK15 pKa = 9.18KK16 pKa = 9.27TGSGKK21 pKa = 10.35LKK23 pKa = 10.13RR24 pKa = 11.84SRR26 pKa = 11.84AFTSHH31 pKa = 6.86RR32 pKa = 11.84FHH34 pKa = 7.48GKK36 pKa = 6.37TKK38 pKa = 9.57KK39 pKa = 9.43QRR41 pKa = 11.84RR42 pKa = 11.84QLRR45 pKa = 11.84KK46 pKa = 9.24SGLVSASDD54 pKa = 3.47MKK56 pKa = 10.71RR57 pKa = 11.84IKK59 pKa = 10.12QQISALL65 pKa = 3.84

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1889

0

1889

586659

30

7073

310.6

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.633 ± 0.059

0.578 ± 0.016

5.559 ± 0.049

7.311 ± 0.075

4.54 ± 0.05

6.143 ± 0.058

1.981 ± 0.029

8.472 ± 0.054

6.731 ± 0.052

10.126 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.029

4.994 ± 0.047

3.392 ± 0.033

4.573 ± 0.05

3.788 ± 0.038

6.275 ± 0.04

5.189 ± 0.058

6.353 ± 0.041

0.853 ± 0.019

3.886 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski