Cacao yellow vein banding virus
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8SRL3|A0A1P8SRL3_9VIRU Uncharacterized protein OS=Cacao yellow vein banding virus OX=2169726 PE=4 SV=1
MM1 pKa = 7.32 EE2 pKa = 5.66 RR3 pKa = 11.84 SSSHH7 pKa = 6.89 RR8 pKa = 11.84 MLTPALPXNLLEE20 pKa = 4.07 DD21 pKa = 4.05 HH22 pKa = 6.24 QWYY25 pKa = 8.72 LQQASNLPQDD35 pKa = 3.97 MQLHH39 pKa = 5.51 XLNEE43 pKa = 4.27 KK44 pKa = 10.32 GAEE47 pKa = 3.96 DD48 pKa = 3.73 CLSYY52 pKa = 11.15 KK53 pKa = 10.29 FXEE56 pKa = 4.45 VSKK59 pKa = 10.53 QLQEE63 pKa = 3.82 FRR65 pKa = 11.84 TMVDD69 pKa = 2.71 RR70 pKa = 11.84 SLAYY74 pKa = 7.73 YY75 pKa = 9.55 TRR77 pKa = 11.84 ISTKK81 pKa = 10.49 DD82 pKa = 3.21 NYY84 pKa = 11.43 AGDD87 pKa = 3.68 HH88 pKa = 6.4 KK89 pKa = 10.97 EE90 pKa = 3.88 ALEE93 pKa = 3.96 YY94 pKa = 10.81 AQGEE98 pKa = 4.51 ILTALTALEE107 pKa = 3.99 KK108 pKa = 10.73 AKK110 pKa = 10.83 CIFIKK115 pKa = 10.48 EE116 pKa = 4.16 RR117 pKa = 11.84 GHH119 pKa = 4.6 VTVLRR124 pKa = 11.84 PP125 pKa = 3.23
Molecular weight: 14.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.9
IPC2_protein 5.868
IPC_protein 5.931
Toseland 6.364
ProMoST 6.313
Dawson 6.224
Bjellqvist 6.198
Wikipedia 6.237
Rodwell 6.211
Grimsley 6.532
Solomon 6.224
Lehninger 6.224
Nozaki 6.478
DTASelect 6.678
Thurlkill 6.707
EMBOSS 6.678
Sillero 6.605
Patrickios 4.101
IPC_peptide 6.249
IPC2_peptide 6.561
IPC2.peptide.svr19 6.532
Protein with the highest isoelectric point:
>tr|A0A1P8SRK1|A0A1P8SRK1_9VIRU Reverse transcriptase OS=Cacao yellow vein banding virus OX=2169726 PE=4 SV=1
MM1 pKa = 7.51 SLADD5 pKa = 4.07 KK6 pKa = 10.7 KK7 pKa = 10.93 ADD9 pKa = 3.65 PSFVEE14 pKa = 5.98 AITQIQRR21 pKa = 11.84 LGEE24 pKa = 4.05 GAGFEE29 pKa = 4.36 VSSGATASKK38 pKa = 10.48 GISTIISQLNVLVYY52 pKa = 10.47 QITALHH58 pKa = 5.67 QKK60 pKa = 9.75 VDD62 pKa = 3.57 ALDD65 pKa = 3.53 ARR67 pKa = 11.84 VRR69 pKa = 11.84 AIQAGKK75 pKa = 8.37 ATDD78 pKa = 3.71 YY79 pKa = 11.43 SDD81 pKa = 3.86 QLEE84 pKa = 4.73 KK85 pKa = 10.52 LTADD89 pKa = 4.78 LSNLSLGTATTKK101 pKa = 10.48 PVVKK105 pKa = 10.45 KK106 pKa = 10.35 KK107 pKa = 9.39 VNKK110 pKa = 9.79 VYY112 pKa = 10.08 FYY114 pKa = 11.06 KK115 pKa = 10.61 DD116 pKa = 3.17 PKK118 pKa = 10.77 QILQEE123 pKa = 4.63 EE124 pKa = 4.49 KK125 pKa = 10.37 TKK127 pKa = 9.78 WQSQKK132 pKa = 11.26
Molecular weight: 14.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.74
IPC2_protein 8.756
IPC_protein 8.668
Toseland 9.619
ProMoST 9.311
Dawson 9.794
Bjellqvist 9.399
Wikipedia 9.926
Rodwell 10.379
Grimsley 9.853
Solomon 9.853
Lehninger 9.823
Nozaki 9.575
DTASelect 9.399
Thurlkill 9.648
EMBOSS 10.014
Sillero 9.706
Patrickios 9.926
IPC_peptide 9.853
IPC2_peptide 7.761
IPC2.peptide.svr19 7.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2388
125
1958
597.0
67.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.077 ± 0.661
1.508 ± 0.277
5.151 ± 0.165
7.58 ± 0.753
3.643 ± 0.389
5.025 ± 1.28
2.429 ± 0.424
6.072 ± 0.825
6.91 ± 1.024
8.752 ± 3.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.136 ± 0.44
4.648 ± 0.548
5.318 ± 1.321
5.402 ± 0.593
5.821 ± 0.604
6.449 ± 0.294
5.653 ± 0.323
5.528 ± 0.529
1.34 ± 0.441
3.224 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here