Flavobacterium phage 11b

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5ZGH3|Q5ZGH3_9CAUD Uncharacterized protein OS=Flavobacterium phage 11b OX=294631 GN=PHG11b_4 PE=4 SV=1
MM1 pKa = 6.91NQEE4 pKa = 4.12EE5 pKa = 4.98NNTQIKK11 pKa = 10.1DD12 pKa = 3.38QDD14 pKa = 3.95GNISKK19 pKa = 9.97PLLAPVIYY27 pKa = 9.59WNKK30 pKa = 10.62LEE32 pKa = 5.12DD33 pKa = 3.38IKK35 pKa = 11.37PNAEE39 pKa = 3.7EE40 pKa = 4.65EE41 pKa = 4.56YY42 pKa = 10.84LVTNSDD48 pKa = 3.13GVIRR52 pKa = 11.84VSYY55 pKa = 10.74FDD57 pKa = 3.59GLNWGYY63 pKa = 10.86LCSVKK68 pKa = 10.64EE69 pKa = 3.99NIIYY73 pKa = 9.89WSILPQSPLL82 pKa = 3.26

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5ZGH0|Q5ZGH0_9CAUD Uncharacterized protein OS=Flavobacterium phage 11b OX=294631 GN=PHG11b_7 PE=4 SV=1
MM1 pKa = 7.33YY2 pKa = 10.62SDD4 pKa = 4.05NNKK7 pKa = 8.88TNNIKK12 pKa = 10.16IMKK15 pKa = 8.2TLEE18 pKa = 4.29KK19 pKa = 10.12IYY21 pKa = 11.01DD22 pKa = 4.07RR23 pKa = 11.84ISWVLFGGIKK33 pKa = 10.62GIFKK37 pKa = 10.61RR38 pKa = 4.22

Molecular weight: 4.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

10863

37

930

167.1

18.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.287 ± 0.481

0.865 ± 0.114

5.588 ± 0.243

6.066 ± 0.4

4.584 ± 0.206

6.518 ± 0.405

1.031 ± 0.128

8.534 ± 0.271

8.543 ± 0.641

9.077 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.961 ± 0.213

7.291 ± 0.347

2.817 ± 0.235

3.59 ± 0.232

3.075 ± 0.202

6.297 ± 0.307

6.812 ± 0.6

5.846 ± 0.314

1.003 ± 0.12

4.216 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski