Carnation mottle virus (isolate China/Shanghai) (CarMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus; Carnation mottle virus

Average proteome isoelectric point is 8.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9Q6X7|CAPSD_CARMS Capsid protein OS=Carnation mottle virus (isolate China/Shanghai) OX=652111 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.72GLPSLLVEE9 pKa = 4.26GVIGCTLVGGLVAVGSAALAVRR31 pKa = 11.84ATIGVVEE38 pKa = 5.01FNRR41 pKa = 11.84EE42 pKa = 4.02CVRR45 pKa = 11.84GARR48 pKa = 11.84RR49 pKa = 11.84IVSSGGRR56 pKa = 11.84CLIVQSPYY64 pKa = 11.2GNPNQGLIRR73 pKa = 11.84GEE75 pKa = 4.14DD76 pKa = 3.53EE77 pKa = 5.18EE78 pKa = 4.91IDD80 pKa = 3.61NVEE83 pKa = 3.81EE84 pKa = 4.23STPEE88 pKa = 3.88EE89 pKa = 4.0LTQLIEE95 pKa = 4.22VKK97 pKa = 10.96AEE99 pKa = 3.77VDD101 pKa = 3.33GKK103 pKa = 10.75EE104 pKa = 3.92VVVSKK109 pKa = 10.81KK110 pKa = 10.33RR111 pKa = 11.84VVNRR115 pKa = 11.84HH116 pKa = 3.72LRR118 pKa = 11.84QRR120 pKa = 11.84FVRR123 pKa = 11.84SIAIEE128 pKa = 4.13AKK130 pKa = 9.42NHH132 pKa = 6.32FGGDD136 pKa = 3.37ISPSKK141 pKa = 11.03ANYY144 pKa = 9.88LSVSKK149 pKa = 10.47FLTGKK154 pKa = 9.73CKK156 pKa = 9.81EE157 pKa = 4.08RR158 pKa = 11.84HH159 pKa = 5.0VVPAHH164 pKa = 5.86TRR166 pKa = 11.84DD167 pKa = 3.79CVSAAMVLVFTPDD180 pKa = 2.82VHH182 pKa = 7.06EE183 pKa = 4.17IRR185 pKa = 11.84MMAGLASDD193 pKa = 3.85AAYY196 pKa = 10.46ARR198 pKa = 11.84KK199 pKa = 9.2IAMASILNRR208 pKa = 11.84KK209 pKa = 6.94GWCWRR214 pKa = 11.84LMVNPLDD221 pKa = 3.81RR222 pKa = 11.84ARR224 pKa = 11.84WWEE227 pKa = 3.83MWCVVNGFDD236 pKa = 4.61SNKK239 pKa = 9.88PVTFPKK245 pKa = 10.05XGGLFYY251 pKa = 11.2LNGVEE256 pKa = 4.25TKK258 pKa = 9.69IRR260 pKa = 11.84RR261 pKa = 11.84GGHH264 pKa = 5.7PSVIEE269 pKa = 4.06VDD271 pKa = 4.24GQCPPRR277 pKa = 11.84EE278 pKa = 3.76RR279 pKa = 11.84KK280 pKa = 10.11LYY282 pKa = 8.08VQNAITTGYY291 pKa = 9.48EE292 pKa = 4.01YY293 pKa = 10.37RR294 pKa = 11.84VHH296 pKa = 5.53NHH298 pKa = 5.51SYY300 pKa = 10.18ANLRR304 pKa = 11.84RR305 pKa = 11.84GLLEE309 pKa = 3.45RR310 pKa = 11.84VFYY313 pKa = 10.17VEE315 pKa = 4.06RR316 pKa = 11.84NKK318 pKa = 10.85EE319 pKa = 4.13LVSCPQPEE327 pKa = 4.1PGSFKK332 pKa = 10.84EE333 pKa = 3.58MGYY336 pKa = 10.1LRR338 pKa = 11.84RR339 pKa = 11.84RR340 pKa = 11.84FHH342 pKa = 6.55RR343 pKa = 11.84VCGNHH348 pKa = 5.21TRR350 pKa = 11.84ISANDD355 pKa = 3.74LVDD358 pKa = 4.53CYY360 pKa = 10.57QGRR363 pKa = 11.84KK364 pKa = 6.25RR365 pKa = 11.84TIYY368 pKa = 10.23EE369 pKa = 3.57NAAASLLDD377 pKa = 3.58RR378 pKa = 11.84AIEE381 pKa = 4.34RR382 pKa = 11.84KK383 pKa = 10.47DD384 pKa = 3.29GDD386 pKa = 3.85LKK388 pKa = 10.58TFIKK392 pKa = 10.72AEE394 pKa = 4.03KK395 pKa = 10.29FNVNLKK401 pKa = 10.13SDD403 pKa = 3.86PAPRR407 pKa = 11.84VIQPRR412 pKa = 11.84SPRR415 pKa = 11.84YY416 pKa = 8.45NVEE419 pKa = 3.57LGRR422 pKa = 11.84YY423 pKa = 6.83LKK425 pKa = 10.42KK426 pKa = 10.69YY427 pKa = 7.28EE428 pKa = 3.77HH429 pKa = 6.89HH430 pKa = 7.16AYY432 pKa = 9.6KK433 pKa = 10.64ALDD436 pKa = 4.48KK437 pKa = 10.56IWGGPTVMKK446 pKa = 10.54GYY448 pKa = 6.47TTEE451 pKa = 4.47EE452 pKa = 4.26VAQHH456 pKa = 6.32IWSAWNQFQTPVAIGFDD473 pKa = 3.33MSRR476 pKa = 11.84FDD478 pKa = 3.3QHH480 pKa = 7.95VSVAALEE487 pKa = 4.71FEE489 pKa = 4.49HH490 pKa = 6.98SCYY493 pKa = 10.15LACFEE498 pKa = 5.13GDD500 pKa = 2.96AHH502 pKa = 7.19LANLLKK508 pKa = 9.92MQLVNHH514 pKa = 6.39GVGFASNGMLRR525 pKa = 11.84YY526 pKa = 7.8TKK528 pKa = 9.9EE529 pKa = 3.9GCRR532 pKa = 11.84MIGDD536 pKa = 3.9MNTALGNCLLACLITKK552 pKa = 9.93HH553 pKa = 5.65LMKK556 pKa = 10.23IRR558 pKa = 11.84SRR560 pKa = 11.84LINNGDD566 pKa = 3.76DD567 pKa = 3.44CVLICEE573 pKa = 4.63RR574 pKa = 11.84TDD576 pKa = 2.74IDD578 pKa = 4.14YY579 pKa = 11.16VVSNLTTGWSRR590 pKa = 11.84FGFNCIAEE598 pKa = 4.26EE599 pKa = 3.86PVYY602 pKa = 10.64EE603 pKa = 4.01MEE605 pKa = 6.04KK606 pKa = 10.26IRR608 pKa = 11.84FCQMAPVFDD617 pKa = 3.99GAGWLMVRR625 pKa = 11.84DD626 pKa = 4.39PLVSMSKK633 pKa = 10.33DD634 pKa = 3.3SHH636 pKa = 8.07SLVHH640 pKa = 6.49WNNEE644 pKa = 3.86TNAKK648 pKa = 9.0QWLKK652 pKa = 10.83SVGMCGLRR660 pKa = 11.84IAGGVPIVQEE670 pKa = 4.92FYY672 pKa = 10.73QKK674 pKa = 10.73YY675 pKa = 9.0VEE677 pKa = 4.14TAGNVKK683 pKa = 9.75EE684 pKa = 4.45NKK686 pKa = 10.23NITEE690 pKa = 4.16KK691 pKa = 10.73SSSGFFMMADD701 pKa = 3.53RR702 pKa = 11.84AKK704 pKa = 10.04RR705 pKa = 11.84GYY707 pKa = 9.89SAVSEE712 pKa = 4.09MCRR715 pKa = 11.84FSFYY719 pKa = 10.43QAFGITPDD727 pKa = 3.41QQIALEE733 pKa = 4.18GEE735 pKa = 4.21IRR737 pKa = 11.84SLTIDD742 pKa = 3.63TNVGPQCEE750 pKa = 4.32AADD753 pKa = 4.06SLWILNRR760 pKa = 11.84KK761 pKa = 6.89YY762 pKa = 10.03QQ763 pKa = 3.42

Molecular weight: 85.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9J5U7|RDRP_CARMS Probable RNA-directed RNA polymerase OS=Carnation mottle virus (isolate China/Shanghai) OX=652111 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.77PSANLHH7 pKa = 6.35PIVLTGVIGLMLLIRR22 pKa = 11.84LRR24 pKa = 11.84CTFTSTFSLPPLVTLNQIIALSFCGLLLNSISRR57 pKa = 11.84AEE59 pKa = 3.86RR60 pKa = 11.84ACYY63 pKa = 10.16YY64 pKa = 10.23NYY66 pKa = 10.88SVDD69 pKa = 3.47SSKK72 pKa = 10.36QQHH75 pKa = 6.02ISISTPNGKK84 pKa = 9.32

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1256

61

763

314.0

34.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.404 ± 0.436

2.389 ± 0.621

4.459 ± 0.588

5.573 ± 1.08

3.662 ± 0.126

7.484 ± 0.587

1.99 ± 0.668

5.414 ± 0.817

5.971 ± 0.78

7.803 ± 1.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.866 ± 0.148

5.016 ± 0.716

4.538 ± 0.742

3.185 ± 0.15

6.608 ± 0.749

6.608 ± 1.101

5.812 ± 1.729

8.758 ± 0.649

1.354 ± 0.338

3.025 ± 0.529
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski