Amino acid dipepetide frequency for Micromonospora sp. NRRL B-16802

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.942AlaAla: 21.942 ± 0.179	0.909AlaCys: 0.909 ± 0.025	8.888AlaAsp: 8.888 ± 0.094	8.173AlaGlu: 8.173 ± 0.09	3.288AlaPhe: 3.288 ± 0.045	14.034AlaGly: 14.034 ± 0.104	2.439AlaHis: 2.439 ± 0.039	4.086AlaIle: 4.086 ± 0.055	2.304AlaLys: 2.304 ± 0.051	14.303AlaLeu: 14.303 ± 0.126	2.387AlaMet: 2.387 ± 0.041	2.326AlaAsn: 2.326 ± 0.042	6.938AlaPro: 6.938 ± 0.073	3.778AlaGln: 3.778 ± 0.049	9.996AlaArg: 9.996 ± 0.077	5.867AlaSer: 5.867 ± 0.054	8.084AlaThr: 8.084 ± 0.088	12.792AlaVal: 12.792 ± 0.109	1.833AlaTrp: 1.833 ± 0.033	2.852AlaTyr: 2.852 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.952CysAla: 0.952 ± 0.026	0.108CysCys: 0.108 ± 0.008	0.475CysAsp: 0.475 ± 0.016	0.299CysGlu: 0.299 ± 0.014	0.195CysPhe: 0.195 ± 0.011	0.865CysGly: 0.865 ± 0.023	0.178CysHis: 0.178 ± 0.011	0.15CysIle: 0.15 ± 0.01	0.088CysLys: 0.088 ± 0.007	0.673CysLeu: 0.673 ± 0.016	0.103CysMet: 0.103 ± 0.008	0.143CysAsn: 0.143 ± 0.009	0.433CysPro: 0.433 ± 0.017	0.2CysGln: 0.2 ± 0.011	0.587CysArg: 0.587 ± 0.021	0.377CysSer: 0.377 ± 0.014	0.435CysThr: 0.435 ± 0.016	0.606CysVal: 0.606 ± 0.02	0.129CysTrp: 0.129 ± 0.009	0.149CysTyr: 0.149 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.75AspAla: 7.75 ± 0.08	0.378AspCys: 0.378 ± 0.017	3.972AspAsp: 3.972 ± 0.053	3.894AspGlu: 3.894 ± 0.055	1.511AspPhe: 1.511 ± 0.034	6.391AspGly: 6.391 ± 0.062	1.251AspHis: 1.251 ± 0.026	1.762AspIle: 1.762 ± 0.037	1.014AspLys: 1.014 ± 0.026	6.867AspLeu: 6.867 ± 0.065	0.763AspMet: 0.763 ± 0.022	1.072AspAsn: 1.072 ± 0.027	4.817AspPro: 4.817 ± 0.06	1.754AspGln: 1.754 ± 0.033	5.449AspArg: 5.449 ± 0.064	2.436AspSer: 2.436 ± 0.043	2.953AspThr: 2.953 ± 0.041	5.277AspVal: 5.277 ± 0.054	1.002AspTrp: 1.002 ± 0.025	1.113AspTyr: 1.113 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	6.407GluAla: 6.407 ± 0.063	0.315GluCys: 0.315 ± 0.013	2.057GluAsp: 2.057 ± 0.035	2.358GluGlu: 2.358 ± 0.046	1.509GluPhe: 1.509 ± 0.031	3.232GluGly: 3.232 ± 0.044	1.34GluHis: 1.34 ± 0.03	2.214GluIle: 2.214 ± 0.036	0.983GluLys: 0.983 ± 0.027	6.391GluLeu: 6.391 ± 0.076	0.865GluMet: 0.865 ± 0.021	0.844GluAsn: 0.844 ± 0.022	3.355GluPro: 3.355 ± 0.046	2.304GluGln: 2.304 ± 0.037	4.985GluArg: 4.985 ± 0.062	2.29GluSer: 2.29 ± 0.042	2.511GluThr: 2.511 ± 0.036	4.655GluVal: 4.655 ± 0.053	0.708GluTrp: 0.708 ± 0.02	1.058GluTyr: 1.058 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.7PheAla: 3.7 ± 0.043	0.245PheCys: 0.245 ± 0.011	2.017PheAsp: 2.017 ± 0.035	1.197PheGlu: 1.197 ± 0.03	0.852PhePhe: 0.852 ± 0.025	3.099PheGly: 3.099 ± 0.049	0.543PheHis: 0.543 ± 0.017	0.74PheIle: 0.74 ± 0.021	0.42PheLys: 0.42 ± 0.017	2.484PheLeu: 2.484 ± 0.041	0.317PheMet: 0.317 ± 0.012	0.632PheAsn: 0.632 ± 0.022	1.338PhePro: 1.338 ± 0.029	0.669PheGln: 0.669 ± 0.021	1.802PheArg: 1.802 ± 0.033	1.35PheSer: 1.35 ± 0.028	1.962PheThr: 1.962 ± 0.032	2.459PheVal: 2.459 ± 0.042	0.435PheTrp: 0.435 ± 0.015	0.586PheTyr: 0.586 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	10.72GlyAla: 10.72 ± 0.091	0.791GlyCys: 0.791 ± 0.026	5.373GlyAsp: 5.373 ± 0.055	4.593GlyGlu: 4.593 ± 0.056	2.761GlyPhe: 2.761 ± 0.045	8.937GlyGly: 8.937 ± 0.1	2.052GlyHis: 2.052 ± 0.036	3.293GlyIle: 3.293 ± 0.042	1.885GlyLys: 1.885 ± 0.04	9.51GlyLeu: 9.51 ± 0.084	1.906GlyMet: 1.906 ± 0.038	1.877GlyAsn: 1.877 ± 0.038	5.216GlyPro: 5.216 ± 0.061	2.963GlyGln: 2.963 ± 0.048	7.886GlyArg: 7.886 ± 0.076	5.057GlySer: 5.057 ± 0.059	5.966GlyThr: 5.966 ± 0.071	8.171GlyVal: 8.171 ± 0.079	1.947GlyTrp: 1.947 ± 0.037	2.417GlyTyr: 2.417 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.307HisAla: 2.307 ± 0.04	0.181HisCys: 0.181 ± 0.009	1.312HisAsp: 1.312 ± 0.032	1.042HisGlu: 1.042 ± 0.024	0.557HisPhe: 0.557 ± 0.019	2.052HisGly: 2.052 ± 0.036	0.618HisHis: 0.618 ± 0.02	0.574HisIle: 0.574 ± 0.017	0.26HisLys: 0.26 ± 0.012	2.434HisLeu: 2.434 ± 0.045	0.277HisMet: 0.277 ± 0.013	0.37HisAsn: 0.37 ± 0.016	1.621HisPro: 1.621 ± 0.031	0.648HisGln: 0.648 ± 0.019	1.982HisArg: 1.982 ± 0.038	0.938HisSer: 0.938 ± 0.026	1.114HisThr: 1.114 ± 0.027	1.615HisVal: 1.615 ± 0.029	0.342HisTrp: 0.342 ± 0.014	0.444HisTyr: 0.444 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	4.925IleAla: 4.925 ± 0.064	0.3IleCys: 0.3 ± 0.012	2.497IleAsp: 2.497 ± 0.044	1.991IleGlu: 1.991 ± 0.036	0.913IlePhe: 0.913 ± 0.023	3.643IleGly: 3.643 ± 0.052	0.558IleHis: 0.558 ± 0.02	1.07IleIle: 1.07 ± 0.032	0.64IleLys: 0.64 ± 0.02	2.484IleLeu: 2.484 ± 0.043	0.463IleMet: 0.463 ± 0.017	0.811IleAsn: 0.811 ± 0.023	1.684IlePro: 1.684 ± 0.033	0.764IleGln: 0.764 ± 0.023	2.441IleArg: 2.441 ± 0.04	1.845IleSer: 1.845 ± 0.033	2.246IleThr: 2.246 ± 0.038	2.984IleVal: 2.984 ± 0.045	0.471IleTrp: 0.471 ± 0.016	0.64IleTyr: 0.64 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	2.126LysAla: 2.126 ± 0.043	0.102LysCys: 0.102 ± 0.008	0.885LysAsp: 0.885 ± 0.027	0.756LysGlu: 0.756 ± 0.026	0.4LysPhe: 0.4 ± 0.015	1.304LysGly: 1.304 ± 0.034	0.322LysHis: 0.322 ± 0.014	0.78LysIle: 0.78 ± 0.028	0.509LysLys: 0.509 ± 0.025	1.73LysLeu: 1.73 ± 0.039	0.323LysMet: 0.323 ± 0.014	0.371LysAsn: 0.371 ± 0.017	1.165LysPro: 1.165 ± 0.026	0.6LysGln: 0.6 ± 0.022	1.229LysArg: 1.229 ± 0.031	0.894LysSer: 0.894 ± 0.025	1.029LysThr: 1.029 ± 0.023	1.61LysVal: 1.61 ± 0.037	0.235LysTrp: 0.235 ± 0.013	0.349LysTyr: 0.349 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	16.315LeuAla: 16.315 ± 0.135	0.728LeuCys: 0.728 ± 0.021	7.007LeuAsp: 7.007 ± 0.069	3.846LeuGlu: 3.846 ± 0.056	2.586LeuPhe: 2.586 ± 0.04	9.219LeuGly: 9.219 ± 0.087	2.238LeuHis: 2.238 ± 0.037	3.607LeuIle: 3.607 ± 0.051	1.436LeuLys: 1.436 ± 0.034	11.739LeuLeu: 11.739 ± 0.12	1.397LeuMet: 1.397 ± 0.03	1.756LeuAsn: 1.756 ± 0.03	6.705LeuPro: 6.705 ± 0.076	1.957LeuGln: 1.957 ± 0.036	9.186LeuArg: 9.186 ± 0.085	5.095LeuSer: 5.095 ± 0.055	7.054LeuThr: 7.054 ± 0.061	9.982LeuVal: 9.982 ± 0.088	1.293LeuTrp: 1.293 ± 0.03	1.745LeuTyr: 1.745 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.112MetAla: 2.112 ± 0.036	0.109MetCys: 0.109 ± 0.008	0.766MetAsp: 0.766 ± 0.02	0.614MetGlu: 0.614 ± 0.02	0.437MetPhe: 0.437 ± 0.017	1.167MetGly: 1.167 ± 0.024	0.315MetHis: 0.315 ± 0.016	0.699MetIle: 0.699 ± 0.024	0.33MetLys: 0.33 ± 0.014	1.878MetLeu: 1.878 ± 0.038	0.284MetMet: 0.284 ± 0.012	0.343MetAsn: 0.343 ± 0.013	1.104MetPro: 1.104 ± 0.027	0.448MetGln: 0.448 ± 0.016	1.413MetArg: 1.413 ± 0.028	1.156MetSer: 1.156 ± 0.027	1.484MetThr: 1.484 ± 0.026	1.364MetVal: 1.364 ± 0.028	0.205MetTrp: 0.205 ± 0.01	0.286MetTyr: 0.286 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.312AsnAla: 2.312 ± 0.041	0.158AsnCys: 0.158 ± 0.011	1.052AsnAsp: 1.052 ± 0.027	0.834AsnGlu: 0.834 ± 0.023	0.547AsnPhe: 0.547 ± 0.019	2.056AsnGly: 2.056 ± 0.042	0.383AsnHis: 0.383 ± 0.016	0.614AsnIle: 0.614 ± 0.018	0.362AsnLys: 0.362 ± 0.014	1.92AsnLeu: 1.92 ± 0.033	0.305AsnMet: 0.305 ± 0.013	0.539AsnAsn: 0.539 ± 0.022	1.518AsnPro: 1.518 ± 0.03	0.617AsnGln: 0.617 ± 0.023	1.407AsnArg: 1.407 ± 0.031	0.978AsnSer: 0.978 ± 0.029	1.129AsnThr: 1.129 ± 0.029	1.544AsnVal: 1.544 ± 0.034	0.339AsnTrp: 0.339 ± 0.016	0.482AsnTyr: 0.482 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	9.35ProAla: 9.35 ± 0.089	0.274ProCys: 0.274 ± 0.013	4.66ProAsp: 4.66 ± 0.056	3.741ProGlu: 3.741 ± 0.043	1.46ProPhe: 1.46 ± 0.029	6.411ProGly: 6.411 ± 0.076	1.17ProHis: 1.17 ± 0.027	1.691ProIle: 1.691 ± 0.032	0.984ProLys: 0.984 ± 0.029	5.393ProLeu: 5.393 ± 0.055	1.018ProMet: 1.018 ± 0.022	1.085ProAsn: 1.085 ± 0.03	4.249ProPro: 4.249 ± 0.07	1.739ProGln: 1.739 ± 0.031	3.804ProArg: 3.804 ± 0.048	3.122ProSer: 3.122 ± 0.047	4.296ProThr: 4.296 ± 0.055	5.842ProVal: 5.842 ± 0.059	0.91ProTrp: 0.91 ± 0.02	1.299ProTyr: 1.299 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.713GlnAla: 3.713 ± 0.048	0.158GlnCys: 0.158 ± 0.01	1.15GlnAsp: 1.15 ± 0.027	1.22GlnGlu: 1.22 ± 0.025	0.808GlnPhe: 0.808 ± 0.024	2.023GlnGly: 2.023 ± 0.034	0.647GlnHis: 0.647 ± 0.02	1.165GlnIle: 1.165 ± 0.028	0.453GlnLys: 0.453 ± 0.018	3.378GlnLeu: 3.378 ± 0.049	0.513GlnMet: 0.513 ± 0.017	0.522GlnAsn: 0.522 ± 0.019	2.037GlnPro: 2.037 ± 0.041	1.245GlnGln: 1.245 ± 0.03	2.798GlnArg: 2.798 ± 0.04	1.276GlnSer: 1.276 ± 0.029	1.413GlnThr: 1.413 ± 0.027	2.984GlnVal: 2.984 ± 0.041	0.545GlnTrp: 0.545 ± 0.018	0.567GlnTyr: 0.567 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.985ArgAla: 9.985 ± 0.09	0.566ArgCys: 0.566 ± 0.019	4.635ArgAsp: 4.635 ± 0.056	4.163ArgGlu: 4.163 ± 0.051	2.378ArgPhe: 2.378 ± 0.041	5.681ArgGly: 5.681 ± 0.055	2.036ArgHis: 2.036 ± 0.035	3.097ArgIle: 3.097 ± 0.05	1.281ArgLys: 1.281 ± 0.031	9.227ArgLeu: 9.227 ± 0.09	1.85ArgMet: 1.85 ± 0.035	1.54ArgAsn: 1.54 ± 0.027	5.284ArgPro: 5.284 ± 0.065	2.83ArgGln: 2.83 ± 0.046	8.45ArgArg: 8.45 ± 0.092	4.145ArgSer: 4.145 ± 0.049	4.867ArgThr: 4.867 ± 0.055	6.422ArgVal: 6.422 ± 0.062	1.626ArgTrp: 1.626 ± 0.027	2.011ArgTyr: 2.011 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	6.616SerAla: 6.616 ± 0.068	0.359SerCys: 0.359 ± 0.015	2.592SerAsp: 2.592 ± 0.038	2.072SerGlu: 2.072 ± 0.038	1.477SerPhe: 1.477 ± 0.031	5.677SerGly: 5.677 ± 0.058	0.891SerHis: 0.891 ± 0.023	1.687SerIle: 1.687 ± 0.031	0.808SerLys: 0.808 ± 0.023	4.335SerLeu: 4.335 ± 0.046	1.056SerMet: 1.056 ± 0.023	0.974SerAsn: 0.974 ± 0.027	3.134SerPro: 3.134 ± 0.044	1.22SerGln: 1.22 ± 0.026	3.767SerArg: 3.767 ± 0.045	2.669SerSer: 2.669 ± 0.046	3.435SerThr: 3.435 ± 0.048	4.257SerVal: 4.257 ± 0.052	0.967SerTrp: 0.967 ± 0.025	1.241SerTyr: 1.241 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	8.744ThrAla: 8.744 ± 0.093	0.432ThrCys: 0.432 ± 0.015	3.856ThrAsp: 3.856 ± 0.05	3.047ThrGlu: 3.047 ± 0.043	1.679ThrPhe: 1.679 ± 0.037	6.651ThrGly: 6.651 ± 0.066	1.075ThrHis: 1.075 ± 0.028	2.114ThrIle: 2.114 ± 0.035	0.973ThrLys: 0.973 ± 0.028	5.851ThrLeu: 5.851 ± 0.071	0.994ThrMet: 0.994 ± 0.025	1.209ThrAsn: 1.209 ± 0.031	4.322ThrPro: 4.322 ± 0.051	1.335ThrGln: 1.335 ± 0.03	4.063ThrArg: 4.063 ± 0.056	3.242ThrSer: 3.242 ± 0.045	3.978ThrThr: 3.978 ± 0.071	6.521ThrVal: 6.521 ± 0.074	0.975ThrTrp: 0.975 ± 0.026	1.28ThrTyr: 1.28 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	12.695ValAla: 12.695 ± 0.102	0.704ValCys: 0.704 ± 0.019	6.021ValAsp: 6.021 ± 0.055	4.872ValGlu: 4.872 ± 0.056	2.34ValPhe: 2.34 ± 0.043	7.702ValGly: 7.702 ± 0.084	1.799ValHis: 1.799 ± 0.034	3.035ValIle: 3.035 ± 0.045	1.414ValLys: 1.414 ± 0.034	10.069ValLeu: 10.069 ± 0.094	1.208ValMet: 1.208 ± 0.03	1.828ValAsn: 1.828 ± 0.037	5.447ValPro: 5.447 ± 0.059	2.147ValGln: 2.147 ± 0.04	7.309ValArg: 7.309 ± 0.068	4.481ValSer: 4.481 ± 0.049	6.2ValThr: 6.2 ± 0.057	9.025ValVal: 9.025 ± 0.093	1.097ValTrp: 1.097 ± 0.028	1.544ValTyr: 1.544 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.716TrpAla: 1.716 ± 0.034	0.159TrpCys: 0.159 ± 0.011	0.748TrpAsp: 0.748 ± 0.022	0.612TrpGlu: 0.612 ± 0.018	0.519TrpPhe: 0.519 ± 0.017	1.008TrpGly: 1.008 ± 0.027	0.397TrpHis: 0.397 ± 0.017	0.55TrpIle: 0.55 ± 0.017	0.283TrpLys: 0.283 ± 0.012	1.954TrpLeu: 1.954 ± 0.036	0.281TrpMet: 0.281 ± 0.013	0.412TrpAsn: 0.412 ± 0.016	0.911TrpPro: 0.911 ± 0.025	0.706TrpGln: 0.706 ± 0.021	1.578TrpArg: 1.578 ± 0.031	1.066TrpSer: 1.066 ± 0.028	1.031TrpThr: 1.031 ± 0.028	1.075TrpVal: 1.075 ± 0.024	0.392TrpTrp: 0.392 ± 0.013	0.396TrpTyr: 0.396 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.833TyrAla: 2.833 ± 0.039	0.165TyrCys: 0.165 ± 0.01	1.463TyrAsp: 1.463 ± 0.03	1.06TyrGlu: 1.06 ± 0.025	0.629TyrPhe: 0.629 ± 0.02	2.167TyrGly: 2.167 ± 0.034	0.433TyrHis: 0.433 ± 0.017	0.443TyrIle: 0.443 ± 0.016	0.296TyrLys: 0.296 ± 0.016	2.269TyrLeu: 2.269 ± 0.035	0.206TyrMet: 0.206 ± 0.012	0.441TyrAsn: 0.441 ± 0.018	1.219TyrPro: 1.219 ± 0.025	0.692TyrGln: 0.692 ± 0.022	1.943TyrArg: 1.943 ± 0.036	0.95TyrSer: 0.95 ± 0.026	1.154TyrThr: 1.154 ± 0.03	1.754TyrVal: 1.754 ± 0.032	0.343TyrTrp: 0.343 ± 0.014	0.437TyrTyr: 0.437 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5527 proteins (1788731 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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