Faeces associated gemycircularvirus 15
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A168MGD9|A0A168MGD9_9VIRU Replication-associated protein OS=Faeces associated gemycircularvirus 15 OX=1843735 PE=3 SV=1
MM1 pKa = 7.42 SNSFRR6 pKa = 11.84 LQARR10 pKa = 11.84 YY11 pKa = 10.17 VLLTYY16 pKa = 8.05 SQCGDD21 pKa = 3.75 LDD23 pKa = 3.26 PWMVCDD29 pKa = 5.19 HH30 pKa = 6.72 LASLHH35 pKa = 6.02 AEE37 pKa = 3.92 CLIGRR42 pKa = 11.84 EE43 pKa = 3.97 NHH45 pKa = 5.59 VDD47 pKa = 3.5 GGIHH51 pKa = 6.05 LHH53 pKa = 6.64 AFVDD57 pKa = 4.11 LGKK60 pKa = 9.14 KK61 pKa = 8.32 TDD63 pKa = 3.36 IRR65 pKa = 11.84 DD66 pKa = 3.35 SRR68 pKa = 11.84 LFDD71 pKa = 4.2 VNGQHH76 pKa = 7.32 PNITPCGRR84 pKa = 11.84 TPQKK88 pKa = 10.31 MLDD91 pKa = 3.71 YY92 pKa = 10.33 AVKK95 pKa = 10.78 DD96 pKa = 3.61 GDD98 pKa = 4.12 VVAGGLDD105 pKa = 3.47 PVLGDD110 pKa = 3.96 EE111 pKa = 4.61 VSSADD116 pKa = 4.15 TVWHH120 pKa = 6.89 RR121 pKa = 11.84 IANAATVDD129 pKa = 4.08 EE130 pKa = 4.85 FWVITRR136 pKa = 11.84 EE137 pKa = 3.96 LAPRR141 pKa = 11.84 ALLCNHH147 pKa = 5.94 QSLRR151 pKa = 11.84 AYY153 pKa = 10.25 AEE155 pKa = 3.53 WHH157 pKa = 5.77 YY158 pKa = 11.06 RR159 pKa = 11.84 PPTLVYY165 pKa = 9.03 EE166 pKa = 4.78 HH167 pKa = 7.17 PCEE170 pKa = 3.95 LRR172 pKa = 11.84 LRR174 pKa = 11.84 ADD176 pKa = 4.23 RR177 pKa = 11.84 LPEE180 pKa = 3.99 LDD182 pKa = 2.91 EE183 pKa = 4.04 WVRR186 pKa = 11.84 EE187 pKa = 4.23 SLSGTGGRR195 pKa = 11.84 PKK197 pKa = 10.75 SLILWGEE204 pKa = 4.09 SKK206 pKa = 10.67 LGKK209 pKa = 8.69 TIWARR214 pKa = 11.84 SLGQHH219 pKa = 6.94 IYY221 pKa = 11.12 CCMQFNVDD229 pKa = 4.01 DD230 pKa = 3.95 VRR232 pKa = 11.84 ANLQEE237 pKa = 3.82 AQYY240 pKa = 11.22 AIFDD244 pKa = 5.01 DD245 pKa = 3.84 IQGNFQFFPSYY256 pKa = 10.36 KK257 pKa = 9.0 GWLGAQHH264 pKa = 6.28 QFTVTDD270 pKa = 3.81 KK271 pKa = 11.3 YY272 pKa = 10.99 KK273 pKa = 11.19 GKK275 pKa = 7.67 TTINWGRR282 pKa = 11.84 PSIWLTNDD290 pKa = 2.94 DD291 pKa = 4.27 PEE293 pKa = 4.58 EE294 pKa = 4.24 VGHH297 pKa = 7.26 VDD299 pKa = 5.37 LNWLRR304 pKa = 11.84 KK305 pKa = 9.22 NCVIVNITEE314 pKa = 4.55 SIVEE318 pKa = 4.04 LVV320 pKa = 3.13
Molecular weight: 36.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.469
IPC2_protein 5.41
IPC_protein 5.436
Toseland 5.804
ProMoST 5.766
Dawson 5.664
Bjellqvist 5.69
Wikipedia 5.664
Rodwell 5.639
Grimsley 5.931
Solomon 5.664
Lehninger 5.652
Nozaki 5.919
DTASelect 6.109
Thurlkill 6.071
EMBOSS 6.033
Sillero 6.008
Patrickios 3.884
IPC_peptide 5.677
IPC2_peptide 6.02
IPC2.peptide.svr19 5.983
Protein with the highest isoelectric point:
>tr|A0A161IBC4|A0A161IBC4_9VIRU RepA OS=Faeces associated gemycircularvirus 15 OX=1843735 PE=3 SV=1
MM1 pKa = 7.97 PIRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 SSRR9 pKa = 11.84 RR10 pKa = 11.84 SAGKK14 pKa = 8.84 RR15 pKa = 11.84 RR16 pKa = 11.84 YY17 pKa = 8.08 PRR19 pKa = 11.84 RR20 pKa = 11.84 YY21 pKa = 8.25 RR22 pKa = 11.84 KK23 pKa = 9.91 APARR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 QTRR33 pKa = 11.84 RR34 pKa = 11.84 MPARR38 pKa = 11.84 RR39 pKa = 11.84 VTRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 ILNVSSIKK52 pKa = 10.52 KK53 pKa = 8.93 RR54 pKa = 11.84 DD55 pKa = 3.48 NMMSAVINPGEE66 pKa = 4.1 ARR68 pKa = 11.84 PTLGPLTTTTGFTSLYY84 pKa = 9.72 IPTARR89 pKa = 11.84 TLAPANDD96 pKa = 3.65 QGEE99 pKa = 4.78 SMRR102 pKa = 11.84 QRR104 pKa = 11.84 QTTYY108 pKa = 10.75 AGWVQRR114 pKa = 11.84 AVQVDD119 pKa = 4.18 IIGGGVWKK127 pKa = 9.79 WRR129 pKa = 11.84 RR130 pKa = 11.84 IVFAYY135 pKa = 9.75 KK136 pKa = 10.36 GGEE139 pKa = 4.23 SLWTGEE145 pKa = 5.67 FPGQWTEE152 pKa = 3.79 PFFSKK157 pKa = 10.32 SVKK160 pKa = 9.75 PDD162 pKa = 3.67 PDD164 pKa = 4.33 DD165 pKa = 4.63 PLPIAPDD172 pKa = 3.39 MVRR175 pKa = 11.84 LIGQPQSAQANAIRR189 pKa = 11.84 DD190 pKa = 4.09 LLWDD194 pKa = 3.39 GHH196 pKa = 6.32 EE197 pKa = 4.33 GVDD200 pKa = 3.76 WSSQYY205 pKa = 7.83 TAKK208 pKa = 10.54 VDD210 pKa = 3.68 TKK212 pKa = 10.35 RR213 pKa = 11.84 VRR215 pKa = 11.84 LMSDD219 pKa = 2.37 KK220 pKa = 10.76 TYY222 pKa = 11.18 VFNPGNEE229 pKa = 4.15 SGMSRR234 pKa = 11.84 TYY236 pKa = 10.76 RR237 pKa = 11.84 FWYY240 pKa = 9.11 PIGHH244 pKa = 6.73 NIVYY248 pKa = 10.56 DD249 pKa = 3.98 DD250 pKa = 5.03 DD251 pKa = 3.79 EE252 pKa = 4.65 WGASAFGRR260 pKa = 11.84 VSPISVQSKK269 pKa = 10.21 LGFGDD274 pKa = 3.57 VYY276 pKa = 10.65 IYY278 pKa = 10.41 DD279 pKa = 3.18 IAYY282 pKa = 9.65 RR283 pKa = 11.84 VVPSSGSVEE292 pKa = 3.81 AQMQFSPEE300 pKa = 3.53 GTYY303 pKa = 10.33 YY304 pKa = 8.87 WHH306 pKa = 6.98 EE307 pKa = 4.02 RR308 pKa = 3.5
Molecular weight: 35.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.838
IPC_protein 10.73
Toseland 10.511
ProMoST 10.423
Dawson 10.687
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 10.804
Grimsley 10.774
Solomon 10.789
Lehninger 10.73
Nozaki 10.482
DTASelect 10.452
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.613
Patrickios 10.409
IPC_peptide 10.789
IPC2_peptide 9.385
IPC2.peptide.svr19 8.495
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
861
233
320
287.0
32.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.433 ± 0.752
2.091 ± 0.933
6.969 ± 0.647
4.53 ± 0.455
2.904 ± 0.237
7.666 ± 0.238
3.02 ± 0.907
4.413 ± 0.569
3.252 ± 0.475
7.898 ± 1.793
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.974 ± 0.442
3.252 ± 0.489
5.575 ± 0.731
3.833 ± 0.413
8.827 ± 1.621
6.736 ± 0.807
5.575 ± 0.3
7.201 ± 0.179
3.136 ± 0.176
3.717 ± 0.512
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here