Mollivirus sibericum

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4JKB9|A0A0M4JKB9_9VIRU Uncharacterized protein OS=Mollivirus sibericum OX=1678078 GN=ml_460 PE=4 SV=1
MM1 pKa = 7.33FVVCTSKK8 pKa = 10.21EE9 pKa = 4.23CSWNGPDD16 pKa = 4.17YY17 pKa = 11.0FLAQTADD24 pKa = 4.03DD25 pKa = 3.84VCEE28 pKa = 4.21MVEE31 pKa = 3.81EE32 pKa = 5.15TYY34 pKa = 9.66WHH36 pKa = 7.08WDD38 pKa = 3.99GICCYY43 pKa = 9.99IYY45 pKa = 10.98AVDD48 pKa = 3.8HH49 pKa = 6.7KK50 pKa = 11.4GDD52 pKa = 3.94GEE54 pKa = 4.16LPEE57 pKa = 4.44YY58 pKa = 10.86LDD60 pKa = 4.76AGDD63 pKa = 4.2EE64 pKa = 4.5FVDD67 pKa = 4.49FLCDD71 pKa = 3.61NEE73 pKa = 4.21EE74 pKa = 4.36HH75 pKa = 6.39IQRR78 pKa = 11.84SGGFRR83 pKa = 11.84KK84 pKa = 10.37NPVIGPAFDD93 pKa = 4.14GLRR96 pKa = 11.84QTLEE100 pKa = 3.92ALEE103 pKa = 4.64KK104 pKa = 10.12PGAPIPDD111 pKa = 3.77TTLLLCYY118 pKa = 10.3RR119 pKa = 11.84CDD121 pKa = 3.58SYY123 pKa = 11.76VWRR126 pKa = 11.84PSATITYY133 pKa = 9.5DD134 pKa = 3.07PRR136 pKa = 11.84YY137 pKa = 10.26NSNDD141 pKa = 3.34TDD143 pKa = 3.88NVPPEE148 pKa = 4.01CSKK151 pKa = 11.17CGFGVTVARR160 pKa = 11.84EE161 pKa = 4.02WGQGG165 pKa = 3.2

Molecular weight: 18.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3SGU7|A0A0M3SGU7_9VIRU Uncharacterized protein OS=Mollivirus sibericum OX=1678078 GN=ml_258 PE=4 SV=1
MM1 pKa = 7.12SAAVVYY7 pKa = 10.11ALLVIIFVVVVACLVAARR25 pKa = 11.84FMAPPRR31 pKa = 11.84TLPNVDD37 pKa = 3.56ARR39 pKa = 11.84RR40 pKa = 11.84CDD42 pKa = 3.39SSPRR46 pKa = 11.84NSVSLAKK53 pKa = 10.03RR54 pKa = 11.84EE55 pKa = 3.89KK56 pKa = 9.84RR57 pKa = 11.84RR58 pKa = 11.84SSRR61 pKa = 11.84RR62 pKa = 11.84AVVEE66 pKa = 3.84SHH68 pKa = 5.38SQRR71 pKa = 11.84DD72 pKa = 3.26RR73 pKa = 11.84ADD75 pKa = 3.37RR76 pKa = 11.84RR77 pKa = 11.84TTPLVVVDD85 pKa = 3.72NCEE88 pKa = 3.83PSLVDD93 pKa = 3.86LAFSASTKK101 pKa = 9.91VRR103 pKa = 11.84AVTNASSAGEE113 pKa = 4.13GASTDD118 pKa = 3.1GWNDD122 pKa = 3.54LAPSTSSRR130 pKa = 11.84SYY132 pKa = 11.38DD133 pKa = 3.25PTPAKK138 pKa = 10.48RR139 pKa = 11.84NIVDD143 pKa = 3.34TMKK146 pKa = 10.58VVTVDD151 pKa = 3.31GRR153 pKa = 11.84RR154 pKa = 11.84CYY156 pKa = 10.26QVGDD160 pKa = 4.03RR161 pKa = 11.84PNQQSGRR168 pKa = 11.84GNYY171 pKa = 9.29SAISIGDD178 pKa = 3.75LPIKK182 pKa = 10.29RR183 pKa = 11.84DD184 pKa = 3.61TLRR187 pKa = 11.84TT188 pKa = 3.64

Molecular weight: 20.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

514

0

514

176753

48

2171

343.9

37.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.086 ± 0.118

2.213 ± 0.071

6.915 ± 0.096

5.473 ± 0.107

2.902 ± 0.055

6.394 ± 0.12

2.58 ± 0.061

3.826 ± 0.06

3.858 ± 0.115

8.732 ± 0.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.052

3.144 ± 0.059

6.069 ± 0.107

3.758 ± 0.085

7.254 ± 0.098

7.801 ± 0.14

5.632 ± 0.082

6.955 ± 0.076

1.602 ± 0.05

2.333 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski