Erysipelothrix phage SE-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4U070|A0A0H4U070_9CAUD HNH endonuclease family protein OS=Erysipelothrix phage SE-1 OX=1675317 PE=4 SV=1
MM1 pKa = 7.3NEE3 pKa = 3.16VMQFVKK9 pKa = 10.85KK10 pKa = 10.59NDD12 pKa = 3.65TTTEE16 pKa = 3.96LYY18 pKa = 10.01IYY20 pKa = 10.9GEE22 pKa = 3.94IRR24 pKa = 11.84KK25 pKa = 9.05QEE27 pKa = 3.73VWEE30 pKa = 3.99VLFDD34 pKa = 3.85VEE36 pKa = 4.66SEE38 pKa = 4.33QVSALSFKK46 pKa = 10.81DD47 pKa = 4.01ALDD50 pKa = 3.6QVEE53 pKa = 4.53TPNLCVRR60 pKa = 11.84INSMGGSVSEE70 pKa = 4.41GLTIYY75 pKa = 11.03NLLNSFSGNVTTIVDD90 pKa = 4.16GFACSIASVIFMAGSKK106 pKa = 10.33RR107 pKa = 11.84IVPEE111 pKa = 3.72SALLMIHH118 pKa = 5.94NAWSKK123 pKa = 11.67AEE125 pKa = 3.97GDD127 pKa = 3.24AHH129 pKa = 7.31AMRR132 pKa = 11.84KK133 pKa = 9.12AADD136 pKa = 4.06DD137 pKa = 4.23LEE139 pKa = 6.65KK140 pKa = 10.0ITQPSINIYY149 pKa = 7.52TSKK152 pKa = 10.02TILNEE157 pKa = 3.63IEE159 pKa = 4.22IRR161 pKa = 11.84NMMDD165 pKa = 3.02KK166 pKa = 9.6EE167 pKa = 4.33TWLEE171 pKa = 3.71AKK173 pKa = 9.49EE174 pKa = 4.03AYY176 pKa = 9.74EE177 pKa = 4.22FGFATVLEE185 pKa = 4.35KK186 pKa = 11.28AEE188 pKa = 4.7ANQCLNDD195 pKa = 3.87RR196 pKa = 11.84YY197 pKa = 10.05LAHH200 pKa = 7.57LIKK203 pKa = 10.45EE204 pKa = 4.19NKK206 pKa = 9.0ALKK209 pKa = 9.74TKK211 pKa = 10.23ILSTNEE217 pKa = 3.36QDD219 pKa = 5.35DD220 pKa = 4.13DD221 pKa = 4.34FFF223 pKa = 6.82

Molecular weight: 25.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4TFE5|A0A0H4TFE5_9CAUD Uncharacterized protein OS=Erysipelothrix phage SE-1 OX=1675317 PE=4 SV=1
MM1 pKa = 7.45SVTSYY6 pKa = 11.39KK7 pKa = 10.35FLKK10 pKa = 9.54QAHH13 pKa = 7.05LDD15 pKa = 3.78YY16 pKa = 10.85EE17 pKa = 4.39LLISLVQRR25 pKa = 11.84YY26 pKa = 7.43QKK28 pKa = 10.84SKK30 pKa = 9.48SSKK33 pKa = 10.04NKK35 pKa = 10.06SIYY38 pKa = 10.33LKK40 pKa = 10.39EE41 pKa = 3.75IQTLINRR48 pKa = 11.84YY49 pKa = 9.66SGVICVPRR57 pKa = 11.84VNTTWRR63 pKa = 11.84LKK65 pKa = 10.43KK66 pKa = 10.26SQYY69 pKa = 11.38LNGKK73 pKa = 7.36WLNQGDD79 pKa = 5.26LIRR82 pKa = 11.84VVGVMVGKK90 pKa = 10.23DD91 pKa = 2.96IIINYY96 pKa = 7.31EE97 pKa = 3.22ISKK100 pKa = 10.04IYY102 pKa = 9.58SSHH105 pKa = 5.86MGIGDD110 pKa = 4.07FFKK113 pKa = 10.88KK114 pKa = 10.6FEE116 pKa = 4.34EE117 pKa = 4.59EE118 pKa = 4.2VSS120 pKa = 3.4

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

10267

58

869

238.8

27.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.607 ± 0.493

0.974 ± 0.159

5.445 ± 0.246

7.159 ± 0.271

4.422 ± 0.259

4.87 ± 0.434

1.938 ± 0.219

8.318 ± 0.327

8.902 ± 0.479

9.37 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.177

6.886 ± 0.356

2.756 ± 0.243

3.506 ± 0.217

4.529 ± 0.29

6.954 ± 0.397

5.084 ± 0.253

6.039 ± 0.405

1.208 ± 0.114

4.675 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski